Construct: sgRNA BRDN0001148600
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGATGGACCTATACACCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMPR1B (658)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77491
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
95123834 |
+ |
BMPR1B |
NNNTGGACCTATACACCACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
5480937 |
- |
ICE1 |
NNNTGAACCTGTACACCACA |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000015.10 |
15 |
52184044 |
+ |
GNB5 |
NNNTGGACCTACACACCACT |
NGG |
2 |
0.5684 |
Tier II |
| 4 |
NC_000014.9 |
14 |
89347301 |
+ |
FOXN3 |
NNNTGGACATAGACACCACA |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000019.10 |
19 |
48552483 |
- |
SULT2B1 |
NNNGGGACCTATACACCACA |
NTG |
2 |
0.0244 |
Tier II |
| 6 |
NC_000015.10 |
15 |
35672939 |
- |
DPH6-DT |
NNNTGTATCTATACACCACA |
NGG |
2 |
0.5833 |
Tier III |
| 7 |
NC_000015.10 |
15 |
52184044 |
+ |
CERNA1 |
NNNTGGACCTACACACCACT |
NGG |
2 |
0.5684 |
Tier III |
| 8 |
NC_000010.11 |
10 |
3537535 |
- |
LOC105376360 |
NNNTGGACCTAGACAGCACA |
NGG |
2 |
0.0769 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
141864554 |
- |
Bmpr1b |
NNNTGGGCCCATACACCACA |
NGG |
2 |
0.3765 |
Tier I |
| 2 |
NC_000072.6 |
6 |
64273995 |
- |
Grid2 |
NNNTGGACCAATACACAACA |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000086.7 |
X |
73879528 |
+ |
L1cam |
NNNTGGATCTATTCACCACA |
NGG |
2 |
0.2625 |
Tier II |
| 4 |
NC_000074.6 |
8 |
14889203 |
- |
Cln8 |
NNNAGGACCTATACACCAAA |
NGG |
2 |
0.2041 |
Tier II |
| 5 |
NC_000076.6 |
10 |
32300739 |
+ |
Nkain2 |
NNNTGGACCTATACACAACA |
NGC |
2 |
0.0104 |
Tier II |
| 6 |
NC_000084.6 |
18 |
23602045 |
- |
Dtna |
NNNTGGCCCTATACACCACA |
NGC |
2 |
0.0097 |
Tier II |
| 7 |
NC_000083.6 |
17 |
57449426 |
+ |
Adgre1 |
NNNTGGACTTATAGACCACA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000072.6 |
6 |
92270526 |
+ |
Gm30688 |
NNNTGGAACTATACACCACT |
NGG |
2 |
0.39 |
Tier III |
Other clones with same target sequence:
(none)
