Construct: sgRNA BRDN0001148603
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAACTCATTACATACGGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K4 (4216)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76511
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
161081006 |
+ |
MAP3K4 |
NNNACTCATTACATACGGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
129769302 |
+ |
GSDMC |
NNNACTCATTACATACAGGG |
NAG |
2 |
0.242 |
Tier II |
| 3 |
NC_000022.11 |
22 |
40469307 |
- |
MRTFA |
NNNACTCATTACTTGCGGGG |
NGG |
2 |
0.195 |
Tier II |
| 4 |
NC_000021.9 |
21 |
42269604 |
- |
ABCG1 |
NNNACTCATTACAGACAGGG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
11922648 |
- |
Prkn |
NNNACTCATTTCATACTGGG |
NGG |
2 |
0.0769 |
Tier II |
| 2 |
NC_000068.7 |
2 |
144526129 |
+ |
Dzank1 |
NNNACTCATTACATAAGGGG |
NGC |
2 |
0.0222 |
Tier II |
| 3 |
NC_000080.6 |
14 |
31775182 |
- |
Ankrd28 |
NNNACTCATTACATATGGGG |
NTG |
2 |
0.012 |
Tier II |
| 4 |
NC_000078.6 |
12 |
70904174 |
+ |
Gm32664 |
NNNACTCATTACATAGGGGG |
NGT |
2 |
0.0025 |
Tier III |
Other clones with same target sequence:
(none)
