Construct: sgRNA BRDN0001148605
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAGTGATGGAACCCCCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ARAF (369)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75662
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
47567021 |
+ |
ARAF |
NNNGTGATGGAACCCCCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
167248574 |
- |
TENM2 |
NNNGTCATGGAACCCCCCGG |
NGG |
1 |
0.6818 |
Tier II |
| 3 |
NC_000005.10 |
5 |
6635559 |
+ |
SRD5A1 |
NNNGTGATGGAACCCCCCTG |
NGG |
1 |
0.6667 |
Tier II |
| 4 |
NC_000006.12 |
6 |
7190857 |
- |
RREB1 |
NNNGTGATGGAACCCCCAAG |
NGG |
2 |
0.3846 |
Tier II |
| 5 |
NC_000013.11 |
13 |
77175072 |
- |
MYCBP2 |
NNNGTGATGGAACACACCGG |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000010.11 |
10 |
7735517 |
- |
ITIH2 |
NNNGTGATGGCACCCCCAGG |
NGG |
2 |
0.2154 |
Tier II |
| 7 |
NC_000006.12 |
6 |
4550418 |
- |
LOC105374894 |
NNNGTGATGGAGCCCCCAGG |
NGG |
2 |
0.3889 |
Tier III |
| 8 |
NC_000001.11 |
1 |
90530502 |
- |
LINC02787 |
NNNGTGATGGAACCCCCTGG |
NGA |
2 |
0.0446 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
97022542 |
+ |
Sp6 |
NNNGTGGTGGAACCACCCGG |
NGG |
2 |
0.1569 |
Tier I |
| 2 |
NC_000084.6 |
18 |
65614153 |
- |
Zfp532 |
NNNGTGATGGAACCCCCAGG |
NGG |
1 |
0.5385 |
Tier II |
| 3 |
NC_000078.6 |
12 |
73610484 |
+ |
Prkch |
NNNGTCCTGGAACCCCCCGG |
NGG |
2 |
0.2983 |
Tier II |
| 4 |
NC_000080.6 |
14 |
54571717 |
+ |
Ajuba |
NNNGTGATGGAACCCGCCGG |
NCG |
2 |
0.0165 |
Tier II |
| 5 |
NC_000069.6 |
3 |
120463566 |
+ |
6530403H02Rik |
NNNGGGATGGACCCCCCCGG |
NGG |
2 |
0.1684 |
Tier III |
| 6 |
NC_000077.6 |
11 |
97022542 |
+ |
Gm11532 |
NNNGTGGTGGAACCACCCGG |
NGG |
2 |
0.1569 |
Tier III |
| 7 |
NC_000083.6 |
17 |
17862866 |
- |
Gm36907 |
NNNGTGATAGAACCCCCCGG |
NGA |
2 |
0.0446 |
Tier III |
Other clones with same target sequence:
(none)
