Construct: sgRNA BRDN0001148607

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTTGATCTTTGGAGCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBK1 (29110)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76363

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 64474309 + TBK1 NNNTGATCTTTGGAGCATTG NGG 0 1.0 Tier I
2 NC_000010.11 10 1633337 - ADARB2 NNNTGAACTATGGAGCATTG NGG 2 0.75 Tier II
3 NC_000023.11 X 12796949 - PRPS2 NNNTGGTCATTGGAGCATTG NGG 2 0.6633 Tier II
4 NC_000009.12 9 15458895 + SNAPC3 NNNTGATCATTGGAGCATTT NGG 2 0.65 Tier II
5 NC_000011.10 11 43402647 + TTC17 NNNTGATCATTGGAGCATTT NGG 2 0.65 Tier II
6 NC_000015.10 15 94448559 + MCTP2 NNNTGATCATTGGAGCATTT NGG 2 0.65 Tier II
7 NC_000023.11 X 106983074 - MORC4 NNNTGATCATTGGAGCATTT NGG 2 0.65 Tier II
8 NC_000020.11 20 2876834 + PTPRA NNNTGATCTTTGGAGAATTC NGG 2 0.4286 Tier II
9 NC_000001.11 1 231347200 - SPRTN NNNTGCTCATTGGAGCATTG NGG 2 0.4221 Tier II
10 NC_000003.12 3 54583630 - CACNA2D3 NNNTGCTCATTGGAGCATTG NGG 2 0.4221 Tier II
11 NC_000020.11 20 61456661 + CDH4 NNNTGATCTTTGGAACACTG NGG 2 0.4034 Tier II
12 NC_000001.11 1 75173980 + LHX8 NNNTGCTCTTTGGAGCATTT NGG 2 0.3182 Tier II
13 NC_000001.11 1 98721195 + SNX7 NNNTGCTCTTTGGAGCATTT NGG 2 0.3182 Tier II
14 NC_000004.12 4 22500011 - ADGRA3 NNNTGCTCTTTGGAGCATTT NGG 2 0.3182 Tier II
15 NC_000008.11 8 29196834 - KIF13B NNNTGCTCTTTGGAGCATTT NGG 2 0.3182 Tier II
16 NC_000008.11 8 106662994 + OXR1 NNNTGCTCTTTGGAGCATTT NGG 2 0.3182 Tier II
17 NC_000016.10 16 25954743 + HS3ST4 NNNTGCTCTTTGGAGCATTT NGG 2 0.3182 Tier II
18 NC_000017.11 17 80793175 + RPTOR NNNTGCTCTTTGGAGCATTT NGG 2 0.3182 Tier II
19 NC_000018.10 18 13077347 + CEP192 NNNTGCTCTTTGGAGCATTT NGG 2 0.3182 Tier II
20 NC_000010.11 10 132561599 + INPP5A NNNTGATCATTGTAGCATTG NGG 2 0.2786 Tier II
21 NC_000010.11 10 70563958 + PALD1 NNNTGATCCTTGGAGGATTG NGG 2 0.142 Tier II
22 NC_000002.12 2 225693270 + NYAP2 NNNTGACCTTTGGAGCTTTG NGG 2 0.0917 Tier II
23 NC_000001.11 1 198168632 + NEK7 NNNTGATCTTTGGAGCCTTC NGG 2 0.0756 Tier II
24 NC_000013.11 13 111271151 + ARHGEF7 NNNTGATGTTTGGAGCATTG NTG 2 0.025 Tier II
25 NC_000004.12 4 76448861 + SHROOM3 NNNTGATCTTTGGAGCATTG NAC 2 0.0 Tier II
26 NC_000014.9 14 22727686 + OXA1L-DT NNNTGATCTTAGGAGCATTT NGG 2 0.525 Tier III
27 NC_000003.12 3 110908792 - LOC151760 NNNTGATATTTGGAGCAATG NGG 2 0.4333 Tier III
28 NC_000006.12 6 21990161 + CASC15 NNNTGATCGTTGGAGCATTT NGG 2 0.4333 Tier III
29 NC_000006.12 6 139845786 - FILNC1 NNNTGAGCTTTGGAGCCTTG NGG 2 0.1038 Tier III
30 NC_000002.12 2 225693270 + LOC105373914 NNNTGACCTTTGGAGCTTTG NGG 2 0.0917 Tier III
31 NC_000020.11 20 60199732 + MIR646HG NNNTGATCTTTGGGGCTTTG NGG 2 0.0622 Tier III
32 NC_000012.12 12 115736650 + LOC105370003 NNNTGATCTTTGCAGCATTG NCG 2 0.0451 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 7002446 + Aurkc NNNTGATCTGTGGTGCATTG NGG 2 0.2667 Tier I
2 NC_000069.6 3 135247561 + Cenpe NNNTGAGCTTTGGAGCATGG NGG 2 0.1471 Tier I
3 NC_000069.6 3 86074588 - Sh3d19 NNNTGATCTTTGGAACATTG NGG 1 0.9412 Tier II
4 NC_000072.6 6 29128166 - Rbm28 NNNTGATCTTTAAAGCATTG NGG 2 0.8615 Tier II
5 NC_000082.6 16 4913152 + Mgrn1 NNNTGGTCTTTGAAGCATTG NGG 2 0.6593 Tier II
6 NC_000073.6 7 49137296 - Nav2 NNNTGATCTTTAGAGCAATG NGG 2 0.6222 Tier II
7 NC_000067.6 1 9615496 - Vxn NNNTGATTTTTGGAGCAATG NGG 2 0.5833 Tier II
8 NC_000070.6 4 106795199 - Acot11 NNNTGGTGTTTGGAGCATTG NGG 2 0.4592 Tier II
9 NC_000070.6 4 20729996 - Nkain3 NNNAGATCTTTGAAGCATTG NGG 2 0.4396 Tier II
10 NC_000069.6 3 107234160 + Prok1 NNNTTATCTATGGAGCATTG NGG 2 0.2571 Tier II
11 NC_000080.6 14 51115772 + Eddm3b NNNTGATCTTCTGAGCATTG NGG 2 0.2564 Tier II
12 NC_000086.7 X 168171008 - Frmpd4 NNNTGATCTTTGGAACATTG NAG 2 0.244 Tier II
13 NC_000083.6 17 30359892 - Btbd9 NNNTGATTTTTGGAGCATTG NAG 2 0.2269 Tier II
14 NC_000077.6 11 36508871 + Tenm2 NNNTGAGCTTTGGAGCAGTG NGG 2 0.1961 Tier II
15 NC_000067.6 1 62776582 - Nrp2 NNNTGATCTTTTGAGCATAG NGG 2 0.1099 Tier II
16 NC_000074.6 8 105597588 + Ripor1 NNNTGATCTTTGGTGCCTTG NGG 2 0.0941 Tier II
17 NC_000079.6 13 113079467 - Cdc20b NNNTGCTCTTTGGAGCTTTG NGG 2 0.0606 Tier II
18 NC_000067.6 1 80596398 - Dock10 NNNTGATCTTTGGAGCATTT NGA 2 0.0486 Tier II
19 NC_000070.6 4 85281295 + Sh3gl2 NNNTGATCTTTGGAGCCTGG NGG 2 0.0441 Tier II
20 NC_000084.6 18 24998653 + Fhod3 NNNTGATCTTTGGACCTTTG NGG 2 0.0364 Tier II
21 NC_000076.6 10 53968051 - Man1a NNNTGATCTTTGGATCATTG NGT 2 0.0023 Tier II
22 NC_000073.6 7 49137296 - 9130015G15Rik NNNTGATCTTTAGAGCAATG NGG 2 0.6222 Tier III
23 NC_000067.6 1 9615496 - 2610203C22Rik NNNTGATTTTTGGAGCAATG NGG 2 0.5833 Tier III
24 NC_000070.6 4 44752534 - Gm12463 NNNTGATCTGTGGTGCATTG NGG 2 0.2667 Tier III
25 NC_000073.6 7 10989071 - Gm7789 NNNTGATCTGTGGTGCATTG NGG 2 0.2667 Tier III
26 NC_000073.6 7 11381274 - Gm45598 NNNTGATCTGTGGTGCATTG NGG 2 0.2667 Tier III
27 NC_000073.6 7 11461415 - Gm7207 NNNTGATCTGTGGTGCATTG NGG 2 0.2667 Tier III
28 NC_000073.6 7 11587049 - Gm7210 NNNTGATCTGTGGTGCATTG NGG 2 0.2667 Tier III
29 NC_000067.6 1 72810597 - Gm30847 NNNTGATCTTTGGAGCACTG NGC 2 0.0095 Tier III
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Other clones with same target sequence:

(none)