Construct: sgRNA BRDN0001148608
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGACAATGGAGAATTGAGG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MLKL (197259)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000016.10 | 16 | 74682776 | + | MLKL | NNNACAATGGAGAATTGAGG | NGG | 0 | 1.0 | Tier I |
2 | NC_000005.10 | 5 | 167331888 | + | TENM2 | NNNACAATGTAAAATTGAGG | NGG | 2 | 0.7583 | Tier II |
3 | NC_000023.11 | X | 3711596 | - | PRKX | NNNACAATGGAAAATTGATG | NGG | 2 | 0.6222 | Tier II |
4 | NC_000017.11 | 17 | 39917015 | + | GSDMB | NNNACAATGGGGAATTGAGA | NGG | 2 | 0.6094 | Tier II |
5 | NC_000001.11 | 1 | 54407449 | + | SSBP3 | NNNACAATGGGAAATTGAGG | NGG | 2 | 0.6067 | Tier II |
6 | NC_000007.14 | 7 | 1555716 | - | TMEM184A | NNNACAAGGGGGAATTGAGG | NGG | 2 | 0.4767 | Tier II |
7 | NC_000012.12 | 12 | 54337687 | + | COPZ1 | NNNAGAATGGAGAATTGAAG | NGG | 2 | 0.4286 | Tier II |
8 | NC_000012.12 | 12 | 72174832 | - | TRHDE | NNNACAGTGGAGAAATGAGG | NGG | 2 | 0.4087 | Tier II |
9 | NC_000016.10 | 16 | 70882995 | + | HYDIN | NNNACAAAGGAGAATTGTGG | NGG | 2 | 0.4 | Tier II |
10 | NC_000011.10 | 11 | 2447063 | - | KCNQ1 | NNNACAAGTGAGAATTGAGG | NGG | 2 | 0.3911 | Tier II |
11 | NC_000003.12 | 3 | 57057914 | + | ARHGEF3 | NNNACAAGGGAGAGTTGAGG | NGG | 2 | 0.3422 | Tier II |
12 | NC_000001.11 | 1 | 179047046 | + | FAM20B | NNNACACTGGAGAATTGAAG | NGG | 2 | 0.3125 | Tier II |
13 | NC_000009.12 | 9 | 83575571 | + | FRMD3 | NNNACTATGGAGAATGGAGG | NGG | 2 | 0.2473 | Tier II |
14 | NC_000014.9 | 14 | 37748620 | - | TTC6 | NNNACAATGGAGAATTAAGG | NAG | 2 | 0.242 | Tier II |
15 | NC_000001.11 | 1 | 49250852 | + | AGBL4 | NNNACCATGGACAATTGAGG | NGG | 2 | 0.2406 | Tier II |
16 | NC_000016.10 | 16 | 83566510 | - | CDH13 | NNNAGAATGGAGAATGGAGG | NGG | 2 | 0.2077 | Tier II |
17 | NC_000006.12 | 6 | 138443594 | + | NHSL1 | NNNACATTGGAGAGTTGAGG | NGG | 2 | 0.2042 | Tier II |
18 | NC_000004.12 | 4 | 40867622 | - | APBB2 | NNNTCAATGGAGAATTTAGG | NGG | 2 | 0.1591 | Tier II |
19 | NC_000015.10 | 15 | 51753333 | + | TMOD2 | NNNACAATGGAGAAATTAGG | NGG | 2 | 0.1447 | Tier II |
20 | NC_000018.10 | 18 | 74917955 | + | ZNF407 | NNNACAATGGTGAATTTAGG | NGG | 2 | 0.0769 | Tier II |
21 | NC_000007.14 | 7 | 138483412 | - | TRIM24 | NNNACAATGGAGAATTTAGG | NAG | 2 | 0.0648 | Tier II |
22 | NC_000011.10 | 11 | 36378959 | - | PRR5L | NNNACAATGGAGAATTGAAG | NGA | 2 | 0.0496 | Tier II |
23 | NC_000009.12 | 9 | 119323736 | - | BRINP1 | NNNACAATGGAGAAGTGAGA | NGG | 2 | 0.0469 | Tier II |
24 | NC_000003.12 | 3 | 100922378 | + | ABI3BP | NNNACAAAGGAGAATTGAGG | NTG | 2 | 0.0312 | Tier II |
25 | NC_000001.11 | 1 | 7123762 | - | CAMTA1 | NNNACAAAGGAGAATTGAGG | NGC | 2 | 0.0178 | Tier II |
26 | NC_000012.12 | 12 | 95807383 | + | LINC02410 | NNNACAATGAAGAATAGAGG | NGG | 2 | 0.8485 | Tier III |
27 | NC_000012.12 | 12 | 95807383 | + | SNRPF-DT | NNNACAATGAAGAATAGAGG | NGG | 2 | 0.8485 | Tier III |
28 | NC_000006.12 | 6 | 6742731 | - | LOC101928004 | NNNACAAAGGAGAATTAAGG | NGG | 2 | 0.7467 | Tier III |
29 | NC_000006.12 | 6 | 80611367 | - | LOC112267962 | NNNATAATGGAGAATTGAGA | NGG | 2 | 0.5966 | Tier III |
30 | NC_000010.11 | 10 | 30702752 | + | SVIL2P | NNNATAATAGAGAATTGAGG | NGG | 2 | 0.4091 | Tier III |
31 | NC_000001.11 | 1 | 70753587 | - | LINC01788 | NNNACCATGGACAATTGAGG | NGG | 2 | 0.2406 | Tier III |
32 | NC_000018.10 | 18 | 74917955 | + | LOC124904323 | NNNACAATGGTGAATTTAGG | NGG | 2 | 0.0769 | Tier III |
33 | NC_000023.11 | X | 39931105 | + | LOC105373180 | NNNACAAGGGAGAAGTGAGG | NGG | 2 | 0.0367 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000074.6 | 8 | 111319842 | + | Mlkl | NNNACAATGGAGAACTCAGG | NGG | 2 | 0.0642 | Tier I |
2 | NC_000070.6 | 4 | 11784957 | + | Cdh17 | NNNAGAATGGAGAATTGAGG | NGG | 1 | 0.6 | Tier II |
3 | NC_000072.6 | 6 | 100846101 | + | Ppp4r2 | NNNACAATGAAGAAATGAGG | NGG | 2 | 0.5404 | Tier II |
4 | NC_000067.6 | 1 | 20243384 | - | Pkhd1 | NNNACAATGGAGAGTTGAGG | NGG | 1 | 0.4667 | Tier II |
5 | NC_000074.6 | 8 | 4760084 | - | Shcbp1 | NNNTCAATGGAGAATTGAGT | NGG | 2 | 0.4455 | Tier II |
6 | NC_000079.6 | 13 | 109113561 | + | Pde4d | NNNACAATGAAGAATTGGGG | NGG | 2 | 0.3733 | Tier II |
7 | NC_000071.6 | 5 | 35394132 | - | Hmx1 | NNNACAGTGGAGAGTTGAGG | NGG | 2 | 0.3294 | Tier II |
8 | NC_000070.6 | 4 | 53591571 | + | Slc44a1 | NNNACAATGGACAAATGAGG | NGG | 2 | 0.3065 | Tier II |
9 | NC_000082.6 | 16 | 43898912 | - | Ccdc191 | NNNAAAATGGAGATTTGAGG | NGG | 2 | 0.3048 | Tier II |
10 | NC_000075.6 | 9 | 60356691 | + | Thsd4 | NNNACAATACAGAATTGAGG | NGG | 2 | 0.2571 | Tier II |
11 | NC_000068.7 | 2 | 101860639 | + | Prr5l | NNNAAAATGGAGAATGGAGG | NGG | 2 | 0.1978 | Tier II |
12 | NC_000073.6 | 7 | 120861924 | - | Eef2k | NNNACAAGGGAGACTTGAGG | NGG | 2 | 0.1571 | Tier II |
13 | NC_000079.6 | 13 | 68631266 | - | Adcy2 | NNNACAATGGAGATCTGAGG | NGG | 2 | 0.1455 | Tier II |
14 | NC_000072.6 | 6 | 38128670 | - | D630045J12Rik | NNNACACTGGAGAACTGAGG | NGG | 2 | 0.1193 | Tier II |
15 | NC_000069.6 | 3 | 30909717 | - | Phc3 | NNNACACTGGAGAATTCAGG | NGG | 2 | 0.1029 | Tier II |
16 | NC_000073.6 | 7 | 83710891 | + | Il16 | NNNACAAGGGAGAATTGAGG | NGA | 2 | 0.0509 | Tier II |
17 | NC_000077.6 | 11 | 65212641 | - | Myocd | NNNACAGTGGAGAAGTGAGG | NGG | 2 | 0.0353 | Tier II |
18 | NC_000078.6 | 12 | 84404750 | - | Entpd5 | NNNACAATGGAGAATGGAGG | NTG | 2 | 0.0135 | Tier II |
19 | NC_000085.6 | 19 | 24176961 | + | Tjp2 | NNNACAATGGAGAATTCAGG | NGC | 2 | 0.0052 | Tier II |
20 | NC_000074.6 | 8 | 22846888 | - | Gm46036 | NNNACAAAAGAGAATTGAGG | NGG | 2 | 0.5143 | Tier III |
21 | NC_000079.6 | 13 | 44574166 | + | Gm40931 | NNNACAAGGGGGAATTGAGG | NGG | 2 | 0.4767 | Tier III |
22 | NC_000072.6 | 6 | 132185985 | + | Gm18912 | NNNACATTGAAGAATTGAGG | NGG | 2 | 0.4083 | Tier III |
23 | NC_000072.6 | 6 | 132269162 | + | Gm43979 | NNNACATTGAAGAATTGAGG | NGG | 2 | 0.4083 | Tier III |
24 | NC_000072.6 | 6 | 53980415 | - | 4921529L05Rik | NNNACAATGGAGAACTGAGG | NGG | 1 | 0.2727 | Tier III |
25 | NC_000073.6 | 7 | 130288680 | - | Gm33555 | NNNACATTGGAGAAATGAGG | NGG | 2 | 0.2533 | Tier III |
26 | NC_000074.6 | 8 | 81038310 | - | Gm9725 | NNNACAATGGAGAATACAGG | NGG | 2 | 0.2139 | Tier III |
27 | NC_000073.6 | 7 | 120861924 | - | Gm15774 | NNNACAAGGGAGACTTGAGG | NGG | 2 | 0.1571 | Tier III |
28 | NC_000073.6 | 7 | 83710891 | + | Phgdh-ps1 | NNNACAAGGGAGAATTGAGG | NGA | 2 | 0.0509 | Tier III |
29 | NC_000082.6 | 16 | 26303195 | - | Gm41436 | NNNACAATGGAGAACTGAGG | NGC | 2 | 0.0061 | Tier III |