Construct: sgRNA BRDN0001148608

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATGACAATGGAGAATTGAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MLKL (197259)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77539

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 74682776 + MLKL NNNACAATGGAGAATTGAGG NGG 0 1.0 Tier I
2 NC_000005.10 5 167331888 + TENM2 NNNACAATGTAAAATTGAGG NGG 2 0.7583 Tier II
3 NC_000023.11 X 3711596 - PRKX NNNACAATGGAAAATTGATG NGG 2 0.6222 Tier II
4 NC_000017.11 17 39917015 + GSDMB NNNACAATGGGGAATTGAGA NGG 2 0.6094 Tier II
5 NC_000001.11 1 54407449 + SSBP3 NNNACAATGGGAAATTGAGG NGG 2 0.6067 Tier II
6 NC_000007.14 7 1555716 - TMEM184A NNNACAAGGGGGAATTGAGG NGG 2 0.4767 Tier II
7 NC_000012.12 12 54337687 + COPZ1 NNNAGAATGGAGAATTGAAG NGG 2 0.4286 Tier II
8 NC_000012.12 12 72174832 - TRHDE NNNACAGTGGAGAAATGAGG NGG 2 0.4087 Tier II
9 NC_000016.10 16 70882995 + HYDIN NNNACAAAGGAGAATTGTGG NGG 2 0.4 Tier II
10 NC_000011.10 11 2447063 - KCNQ1 NNNACAAGTGAGAATTGAGG NGG 2 0.3911 Tier II
11 NC_000003.12 3 57057914 + ARHGEF3 NNNACAAGGGAGAGTTGAGG NGG 2 0.3422 Tier II
12 NC_000001.11 1 179047046 + FAM20B NNNACACTGGAGAATTGAAG NGG 2 0.3125 Tier II
13 NC_000009.12 9 83575571 + FRMD3 NNNACTATGGAGAATGGAGG NGG 2 0.2473 Tier II
14 NC_000014.9 14 37748620 - TTC6 NNNACAATGGAGAATTAAGG NAG 2 0.242 Tier II
15 NC_000001.11 1 49250852 + AGBL4 NNNACCATGGACAATTGAGG NGG 2 0.2406 Tier II
16 NC_000016.10 16 83566510 - CDH13 NNNAGAATGGAGAATGGAGG NGG 2 0.2077 Tier II
17 NC_000006.12 6 138443594 + NHSL1 NNNACATTGGAGAGTTGAGG NGG 2 0.2042 Tier II
18 NC_000004.12 4 40867622 - APBB2 NNNTCAATGGAGAATTTAGG NGG 2 0.1591 Tier II
19 NC_000015.10 15 51753333 + TMOD2 NNNACAATGGAGAAATTAGG NGG 2 0.1447 Tier II
20 NC_000018.10 18 74917955 + ZNF407 NNNACAATGGTGAATTTAGG NGG 2 0.0769 Tier II
21 NC_000007.14 7 138483412 - TRIM24 NNNACAATGGAGAATTTAGG NAG 2 0.0648 Tier II
22 NC_000011.10 11 36378959 - PRR5L NNNACAATGGAGAATTGAAG NGA 2 0.0496 Tier II
23 NC_000009.12 9 119323736 - BRINP1 NNNACAATGGAGAAGTGAGA NGG 2 0.0469 Tier II
24 NC_000003.12 3 100922378 + ABI3BP NNNACAAAGGAGAATTGAGG NTG 2 0.0312 Tier II
25 NC_000001.11 1 7123762 - CAMTA1 NNNACAAAGGAGAATTGAGG NGC 2 0.0178 Tier II
26 NC_000012.12 12 95807383 + LINC02410 NNNACAATGAAGAATAGAGG NGG 2 0.8485 Tier III
27 NC_000012.12 12 95807383 + SNRPF-DT NNNACAATGAAGAATAGAGG NGG 2 0.8485 Tier III
28 NC_000006.12 6 6742731 - LOC101928004 NNNACAAAGGAGAATTAAGG NGG 2 0.7467 Tier III
29 NC_000006.12 6 80611367 - LOC112267962 NNNATAATGGAGAATTGAGA NGG 2 0.5966 Tier III
30 NC_000010.11 10 30702752 + SVIL2P NNNATAATAGAGAATTGAGG NGG 2 0.4091 Tier III
31 NC_000001.11 1 70753587 - LINC01788 NNNACCATGGACAATTGAGG NGG 2 0.2406 Tier III
32 NC_000018.10 18 74917955 + LOC124904323 NNNACAATGGTGAATTTAGG NGG 2 0.0769 Tier III
33 NC_000023.11 X 39931105 + LOC105373180 NNNACAAGGGAGAAGTGAGG NGG 2 0.0367 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 111319842 + Mlkl NNNACAATGGAGAACTCAGG NGG 2 0.0642 Tier I
2 NC_000070.6 4 11784957 + Cdh17 NNNAGAATGGAGAATTGAGG NGG 1 0.6 Tier II
3 NC_000072.6 6 100846101 + Ppp4r2 NNNACAATGAAGAAATGAGG NGG 2 0.5404 Tier II
4 NC_000067.6 1 20243384 - Pkhd1 NNNACAATGGAGAGTTGAGG NGG 1 0.4667 Tier II
5 NC_000074.6 8 4760084 - Shcbp1 NNNTCAATGGAGAATTGAGT NGG 2 0.4455 Tier II
6 NC_000079.6 13 109113561 + Pde4d NNNACAATGAAGAATTGGGG NGG 2 0.3733 Tier II
7 NC_000071.6 5 35394132 - Hmx1 NNNACAGTGGAGAGTTGAGG NGG 2 0.3294 Tier II
8 NC_000070.6 4 53591571 + Slc44a1 NNNACAATGGACAAATGAGG NGG 2 0.3065 Tier II
9 NC_000082.6 16 43898912 - Ccdc191 NNNAAAATGGAGATTTGAGG NGG 2 0.3048 Tier II
10 NC_000075.6 9 60356691 + Thsd4 NNNACAATACAGAATTGAGG NGG 2 0.2571 Tier II
11 NC_000068.7 2 101860639 + Prr5l NNNAAAATGGAGAATGGAGG NGG 2 0.1978 Tier II
12 NC_000073.6 7 120861924 - Eef2k NNNACAAGGGAGACTTGAGG NGG 2 0.1571 Tier II
13 NC_000079.6 13 68631266 - Adcy2 NNNACAATGGAGATCTGAGG NGG 2 0.1455 Tier II
14 NC_000072.6 6 38128670 - D630045J12Rik NNNACACTGGAGAACTGAGG NGG 2 0.1193 Tier II
15 NC_000069.6 3 30909717 - Phc3 NNNACACTGGAGAATTCAGG NGG 2 0.1029 Tier II
16 NC_000073.6 7 83710891 + Il16 NNNACAAGGGAGAATTGAGG NGA 2 0.0509 Tier II
17 NC_000077.6 11 65212641 - Myocd NNNACAGTGGAGAAGTGAGG NGG 2 0.0353 Tier II
18 NC_000078.6 12 84404750 - Entpd5 NNNACAATGGAGAATGGAGG NTG 2 0.0135 Tier II
19 NC_000085.6 19 24176961 + Tjp2 NNNACAATGGAGAATTCAGG NGC 2 0.0052 Tier II
20 NC_000074.6 8 22846888 - Gm46036 NNNACAAAAGAGAATTGAGG NGG 2 0.5143 Tier III
21 NC_000079.6 13 44574166 + Gm40931 NNNACAAGGGGGAATTGAGG NGG 2 0.4767 Tier III
22 NC_000072.6 6 132185985 + Gm18912 NNNACATTGAAGAATTGAGG NGG 2 0.4083 Tier III
23 NC_000072.6 6 132269162 + Gm43979 NNNACATTGAAGAATTGAGG NGG 2 0.4083 Tier III
24 NC_000072.6 6 53980415 - 4921529L05Rik NNNACAATGGAGAACTGAGG NGG 1 0.2727 Tier III
25 NC_000073.6 7 130288680 - Gm33555 NNNACATTGGAGAAATGAGG NGG 2 0.2533 Tier III
26 NC_000074.6 8 81038310 - Gm9725 NNNACAATGGAGAATACAGG NGG 2 0.2139 Tier III
27 NC_000073.6 7 120861924 - Gm15774 NNNACAAGGGAGACTTGAGG NGG 2 0.1571 Tier III
28 NC_000073.6 7 83710891 + Phgdh-ps1 NNNACAAGGGAGAATTGAGG NGA 2 0.0509 Tier III
29 NC_000082.6 16 26303195 - Gm41436 NNNACAATGGAGAACTGAGG NGC 2 0.0061 Tier III
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Other clones with same target sequence:

(none)