Construct: sgRNA BRDN0001148613
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAGACTTACTCCAATGAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK18 (5129)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77881
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
205528093 |
+ |
CDK18 |
NNNGACTTACTCCAATGAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
47226628 |
+ |
CDK16 |
NNNGACATACTCCAATGAGG |
NGG |
1 |
0.875 |
Tier I |
| 3 |
NC_000005.10 |
5 |
137753895 |
- |
HNRNPA0 |
NNNGACCTACTCCAATGTGG |
NGG |
2 |
0.3438 |
Tier I |
| 4 |
NC_000009.12 |
9 |
124266916 |
+ |
NEK6 |
NNNGAATTATTCCAATGAGG |
NGG |
2 |
0.8739 |
Tier II |
| 5 |
NC_000009.12 |
9 |
124266916 |
+ |
LOC124902268 |
NNNGAATTATTCCAATGAGG |
NGG |
2 |
0.8739 |
Tier III |
| 6 |
NC_000005.10 |
5 |
79184330 |
- |
LOC124901011 |
NNNGCCTTAATCCAATGAGG |
NGG |
2 |
0.4333 |
Tier III |
| 7 |
NC_000002.12 |
2 |
77863504 |
- |
LOC101927967 |
NNNGACTTACTGCAATAAGG |
NGG |
2 |
0.4148 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
132117558 |
- |
Cdk18 |
NNNGACCTATTCCAATGAGG |
NGG |
2 |
0.6471 |
Tier I |
| 2 |
NC_000074.6 |
8 |
56547404 |
- |
Cep44 |
NNNGAATTACCCCAATGAGG |
NGG |
2 |
0.619 |
Tier I |
| 3 |
NC_000075.6 |
9 |
56926141 |
- |
Snx33 |
NNNGACTTACTCCATTGAGA |
NGG |
2 |
0.1875 |
Tier I |
| 4 |
NC_000072.6 |
6 |
39006389 |
- |
Tbxas1 |
NNNAACTTACTCCAGTGAGG |
NGG |
2 |
0.585 |
Tier II |
| 5 |
NC_000067.6 |
1 |
93199927 |
+ |
Crocc2 |
NNNGACATACTCCTATGAGG |
NGG |
2 |
0.4667 |
Tier II |
| 6 |
NC_000085.6 |
19 |
30894959 |
- |
Prkg1 |
NNNGACTTCCTCCAATGATG |
NGG |
2 |
0.381 |
Tier II |
| 7 |
NC_000068.7 |
2 |
65135908 |
- |
Cobll1 |
NNNGACTTACTCCACTGAGG |
NGG |
1 |
0.2727 |
Tier II |
| 8 |
NC_000069.6 |
3 |
83907041 |
+ |
Tmem131l |
NNNGACTTTCTCCAAGGAGG |
NGG |
2 |
0.2077 |
Tier II |
| 9 |
NC_000069.6 |
3 |
116372306 |
- |
Cdc14a |
NNNGACTTCCTCCAATGAGG |
NAG |
2 |
0.1481 |
Tier II |
| 10 |
NC_000078.6 |
12 |
51060696 |
- |
1700008C04Rik |
NNNGACTTTCTCCAATGAGT |
NGG |
2 |
0.42 |
Tier III |
| 11 |
NC_000078.6 |
12 |
33127124 |
- |
F730043M19Rik |
NNNGACTTACCCAAATGAGG |
NGG |
2 |
0.2564 |
Tier III |
Other clones with same target sequence:
(none)
