Construct: sgRNA BRDN0001148616

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAACGGCTCCGGACCACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HKDC1 (80201)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76827

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 69247554 + HKDC1 NNNACGGCTCCGGACCACAG NGG 0 1.0 Tier I
2 NC_000011.10 11 64826753 - CDC42BPG NNNGCGGCTCCGGCCCACAG NGG 2 0.1339 Tier I
3 NC_000019.10 19 15442892 - WIZ NNNAGGGCTCAGGACCACAG NGG 2 0.45 Tier II
4 NC_000020.11 20 62464567 - GATA5 NNNAAGGCTCCGGACCACAC NGG 2 0.2449 Tier II
5 NC_000022.11 22 24494640 + ADORA2A-AS1 NNNAAGGCTCCGGACGACAG NGG 2 0.0879 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 62401764 - Hkdc1 NNNCCGGCTCCGCACCACAG NGG 2 0.1486 Tier I
2 NC_000068.7 2 130656560 - Ddrgk1 NNNACGGCCCAGGACCACAG NGG 2 0.6923 Tier II
3 NC_000070.6 4 44680815 - Pax5 NNNAGGGCTCCAGACCACAG NGG 2 0.56 Tier II
4 NC_000068.7 2 92994412 - Prdm11 NNNACGGCTCCGAACGACAG NGG 2 0.142 Tier II
5 NC_000075.6 9 61327014 - Gm34264 NNNATGGCTCCAGACCACAG NGG 2 0.5939 Tier III
Download CSV

Other clones with same target sequence:

(none)