Construct: sgRNA BRDN0001148616
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAACGGCTCCGGACCACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HKDC1 (80201)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76827
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
69247554 |
+ |
HKDC1 |
NNNACGGCTCCGGACCACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
64826753 |
- |
CDC42BPG |
NNNGCGGCTCCGGCCCACAG |
NGG |
2 |
0.1339 |
Tier I |
| 3 |
NC_000019.10 |
19 |
15442892 |
- |
WIZ |
NNNAGGGCTCAGGACCACAG |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000020.11 |
20 |
62464567 |
- |
GATA5 |
NNNAAGGCTCCGGACCACAC |
NGG |
2 |
0.2449 |
Tier II |
| 5 |
NC_000022.11 |
22 |
24494640 |
+ |
ADORA2A-AS1 |
NNNAAGGCTCCGGACGACAG |
NGG |
2 |
0.0879 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
62401764 |
- |
Hkdc1 |
NNNCCGGCTCCGCACCACAG |
NGG |
2 |
0.1486 |
Tier I |
| 2 |
NC_000068.7 |
2 |
130656560 |
- |
Ddrgk1 |
NNNACGGCCCAGGACCACAG |
NGG |
2 |
0.6923 |
Tier II |
| 3 |
NC_000070.6 |
4 |
44680815 |
- |
Pax5 |
NNNAGGGCTCCAGACCACAG |
NGG |
2 |
0.56 |
Tier II |
| 4 |
NC_000068.7 |
2 |
92994412 |
- |
Prdm11 |
NNNACGGCTCCGAACGACAG |
NGG |
2 |
0.142 |
Tier II |
| 5 |
NC_000075.6 |
9 |
61327014 |
- |
Gm34264 |
NNNATGGCTCCAGACCACAG |
NGG |
2 |
0.5939 |
Tier III |
Other clones with same target sequence:
(none)
