Construct: sgRNA BRDN0001148617
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTTGAAAAAGGGTCTTGAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME7 (29922)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76496
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
169303160 |
+ |
NME7 |
NNNTGAAAAAGGGTCTTGAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
74906249 |
- |
XRRA1 |
NNNTGAAAAAGGATGTTGAC |
NGG |
2 |
0.0462 |
Tier I |
| 3 |
NC_000002.12 |
2 |
24913833 |
+ |
ADCY3 |
NNNTGAAAAAAGGTCTTAAC |
NGG |
2 |
0.6923 |
Tier II |
| 4 |
NC_000010.11 |
10 |
6505946 |
- |
PRKCQ |
NNNTGAAAAAGGGTCTTGTC |
NGG |
1 |
0.5385 |
Tier II |
| 5 |
NC_000006.12 |
6 |
6146690 |
- |
F13A1 |
NNNTGAAAAAGGGACTTCAC |
NGG |
2 |
0.2948 |
Tier II |
| 6 |
NC_000010.11 |
10 |
110774077 |
- |
RBM20 |
NNNTGAATAAGGGACTTGAC |
NGG |
2 |
0.2653 |
Tier II |
| 7 |
NC_000008.11 |
8 |
65624098 |
- |
ARMC1 |
NNNTGAAACAGGGTCTTGGC |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000013.11 |
13 |
23742836 |
+ |
MIPEP |
NNNTGATAAAGGGTCTTCAC |
NGG |
2 |
0.2083 |
Tier II |
| 9 |
NC_000007.14 |
7 |
107492796 |
- |
COG5 |
NNNTGCAAAAGGGTCTTGGC |
NGG |
2 |
0.1705 |
Tier II |
| 10 |
NC_000002.12 |
2 |
212073435 |
+ |
ERBB4 |
NNNTTAATAAGGGTCTTGAC |
NGG |
2 |
0.1286 |
Tier II |
| 11 |
NC_000005.10 |
5 |
112907828 |
+ |
REEP5 |
NNNTTAAAAAGGGTATTGAC |
NGG |
2 |
0.0667 |
Tier II |
| 12 |
NC_000002.12 |
2 |
88740888 |
+ |
RPIA |
NNNTGAAAACGGGTCTTGAG |
NGG |
2 |
0.0196 |
Tier II |
| 13 |
NC_000007.14 |
7 |
81735403 |
+ |
HGF |
NNNTGAAAAAGGGTATTGAC |
NGC |
2 |
0.0049 |
Tier II |
| 14 |
NC_000020.11 |
20 |
18313806 |
+ |
ZNF133 |
NNNTGAAAAAGGGTCTTGAG |
NGA |
2 |
0.0041 |
Tier II |
| 15 |
NC_000023.11 |
X |
3611578 |
- |
PRKX |
NNNTGAAAAAGGGTTTTGAC |
NGT |
2 |
0.0011 |
Tier II |
| 16 |
NC_000004.12 |
4 |
38512126 |
- |
LINC01258 |
NNNTGAAAGAGGGTCATGAC |
NGG |
2 |
0.6061 |
Tier III |
| 17 |
NC_000004.12 |
4 |
38512126 |
- |
LINC01259 |
NNNTGAAAGAGGGTCATGAC |
NGG |
2 |
0.6061 |
Tier III |
| 18 |
NC_000014.9 |
14 |
44252531 |
+ |
LINC02307 |
NNNGGAAAAAGGGTCATGAC |
NGG |
2 |
0.5682 |
Tier III |
| 19 |
NC_000009.12 |
9 |
1351925 |
+ |
LOC102723803 |
NNNTAAAAAAGGGACTTGAC |
NGG |
2 |
0.5365 |
Tier III |
| 20 |
NC_000008.11 |
8 |
93339187 |
- |
LOC105375642 |
NNNGGAAATAGGGTCTTGAC |
NGG |
2 |
0.375 |
Tier III |
| 21 |
NC_000008.11 |
8 |
93339187 |
- |
LOC107986956 |
NNNGGAAATAGGGTCTTGAC |
NGG |
2 |
0.375 |
Tier III |
| 22 |
NC_000012.12 |
12 |
80764849 |
- |
LINC01490 |
NNNTGGAAAATGGTCTTGAC |
NGG |
2 |
0.2747 |
Tier III |
| 23 |
NC_000012.12 |
12 |
64924909 |
- |
LINC02389 |
NNNTGAAAAGGGGTCTTGAC |
NTG |
2 |
0.0216 |
Tier III |
| 24 |
NC_000012.12 |
12 |
64924909 |
- |
LINC02231 |
NNNTGAAAAGGGGTCTTGAC |
NTG |
2 |
0.0216 |
Tier III |
| 25 |
NC_000020.11 |
20 |
18313806 |
+ |
ZNF133-AS1 |
NNNTGAAAAAGGGTCTTGAG |
NGA |
2 |
0.0041 |
Tier III |
| 26 |
NC_000024.10 |
Y |
7372493 |
+ |
PRKY |
NNNTGAAAAAGGGTTTTGAC |
NGT |
2 |
0.0011 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
62717391 |
+ |
Kcnh7 |
NNNTGAAAGAGAGTCTTGAC |
NGG |
2 |
0.6222 |
Tier II |
| 2 |
NC_000072.6 |
6 |
17868076 |
- |
St7 |
NNNTGAAAATGGGACTTGAC |
NGG |
2 |
0.5462 |
Tier II |
| 3 |
NC_000077.6 |
11 |
109532038 |
+ |
Arsg |
NNNTGAAAATGGGTCTTGAA |
NGG |
2 |
0.4412 |
Tier II |
| 4 |
NC_000068.7 |
2 |
163609566 |
- |
Ttpal |
NNNTGAAGAAGGGTCTAGAC |
NGG |
2 |
0.3911 |
Tier II |
| 5 |
NC_000077.6 |
11 |
107394603 |
+ |
Pitpnc1 |
NNNTGAAGAATGGTCTTGAC |
NGG |
2 |
0.2821 |
Tier II |
| 6 |
NC_000076.6 |
10 |
42476263 |
- |
Afg1l |
NNNTGAAAGAGGGTCTTGGC |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000068.7 |
2 |
152248705 |
+ |
Csnk2a1 |
NNNTGAAACAGGGTCTTGAT |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000075.6 |
9 |
56354723 |
+ |
Peak1 |
NNNTGAAAAAGGTTCTTGGC |
NGG |
2 |
0.1125 |
Tier II |
| 9 |
NC_000078.6 |
12 |
116825325 |
- |
Ptprn2 |
NNNAGAAAAAGGGTTTTGAC |
NGG |
2 |
0.0317 |
Tier II |
| 10 |
NC_000069.6 |
3 |
38460640 |
- |
Ankrd50 |
NNNTGAAAAAGGGTCCTGAC |
NGT |
2 |
0.0108 |
Tier II |
| 11 |
NC_000073.6 |
7 |
139278441 |
+ |
Gm32486 |
NNNTGAAAAGGAGTCTTGAC |
NGG |
2 |
0.5185 |
Tier III |
| 12 |
NC_000068.7 |
2 |
128545208 |
- |
Gm39928 |
NNNAGAGAAAGGGTCTTGAC |
NGG |
2 |
0.3361 |
Tier III |
Other clones with same target sequence:
(none)
