Construct: sgRNA BRDN0001148618
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAACCCACCCCCTAACGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROR2 (4920)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77942
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
91757459 |
- |
ROR2 |
NNNACCCACCCCCTAACGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
88469858 |
+ |
ZFPM1 |
NNNCCCCACCCCCTAACGTG |
NGG |
1 |
0.3529 |
Tier II |
| 3 |
NC_000020.11 |
20 |
483566 |
+ |
CSNK2A1 |
NNNACCAACCCCCTAAAGTG |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000012.12 |
12 |
26607259 |
+ |
ITPR2 |
NNNACCCACCCCCTAACTTC |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000022.11 |
22 |
33732925 |
+ |
LARGE1 |
NNNACCCACCCCCTCACCTG |
NGG |
2 |
0.1299 |
Tier II |
| 6 |
NC_000022.11 |
22 |
33732925 |
+ |
LARGE-AS1 |
NNNACCCACCCCCTCACCTG |
NGG |
2 |
0.1299 |
Tier III |
| 7 |
NC_000005.10 |
5 |
36246784 |
+ |
LOC124900962 |
NNNACCCACCCCCTAGCCTG |
NGG |
2 |
0.0916 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
76287665 |
+ |
Mthfd1 |
NNNATCCACCTCCTAACGTG |
NGG |
2 |
0.1958 |
Tier II |
| 2 |
NC_000070.6 |
4 |
82990895 |
- |
Frem1 |
NNNACCCACCCCCTAATGTG |
NGA |
2 |
0.0324 |
Tier II |
| 3 |
NC_000078.6 |
12 |
108696255 |
+ |
Degs2 |
NNNACCCACCGCCTAAGGTG |
NGG |
2 |
0.0147 |
Tier II |
| 4 |
NC_000078.6 |
12 |
108696255 |
+ |
Gm33467 |
NNNACCCACCGCCTAAGGTG |
NGG |
2 |
0.0147 |
Tier III |
Other clones with same target sequence:
(none)
