Construct: sgRNA BRDN0001148621
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCCGTACTACACTCAGGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KC1 (26750)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75979
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
213117393 |
- |
RPS6KC1 |
NNNCGTACTACACTCAGGAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
42147211 |
- |
PLA2G4F |
NNNAGTACTACACTCAGGAA |
NTG |
2 |
0.0328 |
Tier I |
| 3 |
NC_000003.12 |
3 |
28444221 |
- |
ZCWPW2 |
NNNCGTGCTACACTCAAGAA |
NGG |
2 |
0.6588 |
Tier II |
| 4 |
NC_000009.12 |
9 |
83700364 |
+ |
UBQLN1 |
NNNCATACTACACTCAGTAA |
NGG |
2 |
0.5778 |
Tier II |
| 5 |
NC_000015.10 |
15 |
72583601 |
- |
ARIH1 |
NNNTGTACTACACTCAGGAA |
NAG |
2 |
0.2074 |
Tier II |
| 6 |
NC_000001.11 |
1 |
231572257 |
- |
TSNAX-DISC1 |
NNNAGTATTACACTCAGGAA |
NGG |
2 |
0.7368 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
91824360 |
- |
Ambra1 |
NNNAGTCCTACACTCAGGAA |
NGG |
2 |
0.3684 |
Tier II |
| 2 |
NC_000072.6 |
6 |
28787765 |
+ |
Snd1 |
NNNCGTCCTAAACTCAGGAA |
NGG |
2 |
0.3281 |
Tier II |
| 3 |
NC_000079.6 |
13 |
8476019 |
- |
Adarb2 |
NNNCGCACTACACTTAGGAA |
NGG |
2 |
0.0606 |
Tier II |
| 4 |
NC_000077.6 |
11 |
33793287 |
- |
Kcnip1 |
NNNTGTACTACACTCAGGAA |
NGA |
2 |
0.0556 |
Tier II |
| 5 |
NC_000071.6 |
5 |
13797420 |
- |
4930568A13Rik |
NNNTTTACTACACTCAGGAA |
NGG |
2 |
0.24 |
Tier III |
| 6 |
NC_000069.6 |
3 |
58730156 |
- |
4930593A02Rik |
NNNCTTACTACCCTCAGGAA |
NGG |
2 |
0.0789 |
Tier III |
| 7 |
NC_000068.7 |
2 |
30662034 |
- |
Gm14486 |
NNNCTTACTACACTCAGGCA |
NGG |
2 |
0.0621 |
Tier III |
Other clones with same target sequence:
(none)
