Construct: sgRNA BRDN0001148622
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGGAATTGGTCAGACGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDC42BPB (9578)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77776
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
102968602 |
- |
CDC42BPB |
NNNGGAATTGGTCAGACGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
17881978 |
- |
TOM1L2 |
NNNAGAATTGGTCAGACTTG |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000016.10 |
16 |
16118110 |
- |
ABCC1 |
NNNGGAATTGGACAGACTTG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000009.12 |
9 |
113576685 |
+ |
RGS3 |
NNNGGGAGTGGTCAGACGTG |
NGG |
2 |
0.5238 |
Tier II |
| 5 |
NC_000022.11 |
22 |
16970328 |
+ |
GAB4 |
NNNGGAATTGGGCAGACCTG |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000009.12 |
9 |
133535627 |
+ |
ADAMTSL2 |
NNNGGAATTGGCCAGGCGTG |
NGG |
2 |
0.1822 |
Tier II |
| 7 |
NC_000001.11 |
1 |
75191292 |
+ |
LHX8 |
NNNTGACTTGGTCAGACGTG |
NGG |
2 |
0.1591 |
Tier II |
| 8 |
NC_000002.12 |
2 |
72339468 |
- |
EXOC6B |
NNNGGAATTGGTCAAAGGTG |
NGG |
2 |
0.0554 |
Tier II |
| 9 |
NC_000003.12 |
3 |
174097796 |
+ |
NLGN1 |
NNNGGAAGTGGTCAGAGGTG |
NGG |
2 |
0.0431 |
Tier II |
| 10 |
NC_000006.12 |
6 |
73165995 |
+ |
KCNQ5 |
NNNGGAAGTGGTCAGAGGTG |
NGG |
2 |
0.0431 |
Tier II |
| 11 |
NC_000016.10 |
16 |
56965848 |
- |
CETP |
NNNGGACTTGGTCAGAGGTG |
NGG |
2 |
0.0257 |
Tier II |
| 12 |
NC_000001.11 |
1 |
50170521 |
- |
ELAVL4 |
NNNGGAGTTGGTCAGACGTG |
NGT |
2 |
0.0114 |
Tier II |
| 13 |
NC_000017.11 |
17 |
75364536 |
- |
GRB2 |
NNNGGAATTGGTCAGACCTG |
NGT |
2 |
0.0077 |
Tier II |
| 14 |
NC_000009.12 |
9 |
33165523 |
- |
B4GALT1 |
NNNGGAATTGGTCAGACGTG |
NAC |
2 |
0.0 |
Tier II |
| 15 |
NC_000010.11 |
10 |
9256733 |
+ |
LOC101928272 |
NNNGGAATTGGTCAGAGATG |
NGG |
2 |
0.0407 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
67970243 |
+ |
Ldlrad4 |
NNNGGAATTGGACAGACATG |
NGG |
2 |
0.5538 |
Tier II |
| 2 |
NC_000076.6 |
10 |
117772895 |
- |
Nup107 |
NNNAGAATTGTTCAGACGTG |
NGG |
2 |
0.3462 |
Tier II |
| 3 |
NC_000084.6 |
18 |
45435831 |
+ |
Kcnn2 |
NNNGGAATTGATCAGACGTG |
NGC |
2 |
0.0222 |
Tier II |
| 4 |
NC_000073.6 |
7 |
37962873 |
- |
Uri1 |
NNNGGAATTGGTGAGACGTG |
NGA |
2 |
0.0095 |
Tier II |
| 5 |
NC_000073.6 |
7 |
122413561 |
- |
Prkcb |
NNNGGAATTGGTGAGAGGTG |
NGG |
2 |
0.008 |
Tier II |
| 6 |
NC_000077.6 |
11 |
62977759 |
- |
Cdrt4 |
NNNGGAATTCGTCAGCCGTG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000068.7 |
2 |
34101049 |
+ |
C230014O12Rik |
NNNGGGATTGGTCAGACCTG |
NGG |
2 |
0.3401 |
Tier III |
| 8 |
NC_000084.6 |
18 |
45435831 |
+ |
Gm31907 |
NNNGGAATTGATCAGACGTG |
NGC |
2 |
0.0222 |
Tier III |
| 9 |
NC_000068.7 |
2 |
166163722 |
+ |
Gm30320 |
NNNGGAATTGGTCAGACATG |
NGC |
2 |
0.0154 |
Tier III |
Other clones with same target sequence:
(none)
