Construct: sgRNA BRDN0001148623
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAATTTCATCACGAAAACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL3 (51265)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75607
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
134360076 |
- |
CDKL3 |
NNNATTTCATCACGAAAACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
71145372 |
+ |
TSPAN8 |
NNNATTACATCATGAAAACC |
NGG |
2 |
0.6125 |
Tier II |
| 3 |
NC_000023.11 |
X |
7058453 |
+ |
PUDP |
NNNATCTCATCACCAAAACC |
NGG |
2 |
0.3896 |
Tier II |
| 4 |
NC_000007.14 |
7 |
122342395 |
+ |
CADPS2 |
NNNATTTCATCACAAAAATC |
NGG |
2 |
0.3462 |
Tier II |
| 5 |
NC_000023.11 |
X |
138717207 |
+ |
FGF13 |
NNNATTTAATCAAGAAAACC |
NGG |
2 |
0.25 |
Tier II |
| 6 |
NC_000004.12 |
4 |
75503786 |
+ |
RCHY1 |
NNNATTTCATGACAAAAACC |
NGG |
2 |
0.1875 |
Tier II |
| 7 |
NC_000013.11 |
13 |
113010275 |
- |
MCF2L |
NNNATTTCATCCTGAAAACC |
NGG |
2 |
0.1842 |
Tier II |
| 8 |
NC_000003.12 |
3 |
173513648 |
- |
NLGN1 |
NNNATTTCATTAAGAAAACC |
NGG |
2 |
0.1183 |
Tier II |
| 9 |
NC_000002.12 |
2 |
229796983 |
+ |
TRIP12 |
NNNATTTCATGAGGAAAACC |
NGG |
2 |
0.0341 |
Tier II |
| 10 |
NC_000021.9 |
21 |
28774947 |
+ |
N6AMT1 |
NNNATTTCATGAGGAAAACC |
NGG |
2 |
0.0341 |
Tier II |
| 11 |
NC_000015.10 |
15 |
39641630 |
- |
FSIP1 |
NNNATTTCATCAGGTAAACC |
NGG |
2 |
0.0273 |
Tier II |
| 12 |
NC_000018.10 |
18 |
25131240 |
+ |
ZNF521 |
NNNATTTCATCAAGAAAACC |
NTG |
2 |
0.015 |
Tier II |
| 13 |
NC_000013.11 |
13 |
113010275 |
- |
LOC107984591 |
NNNATTTCATCCTGAAAACC |
NGG |
2 |
0.1842 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
167214251 |
+ |
Ptgis |
NNNATTTCATCACGAAAACA |
NGG |
1 |
0.5 |
Tier II |
| 2 |
NC_000072.6 |
6 |
52185495 |
+ |
Hoxa3 |
NNNACTTCATCACCAAAACC |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000067.6 |
1 |
129539938 |
- |
Thsd7b |
NNNATTTCATCACCAAAACA |
NGG |
2 |
0.2143 |
Tier II |
| 4 |
NC_000086.7 |
X |
43427487 |
- |
Tenm1 |
NNNATTTCATCCTGAAAACC |
NGG |
2 |
0.1842 |
Tier II |
| 5 |
NC_000086.7 |
X |
84060284 |
+ |
Dmd |
NNNTTTTCATCACTAAAACC |
NGG |
2 |
0.1697 |
Tier II |
| 6 |
NC_000086.7 |
X |
10103512 |
+ |
Srpx |
NNNATTTCATCAAGAAAAAC |
NGG |
2 |
0.1648 |
Tier II |
| 7 |
NC_000067.6 |
1 |
25340800 |
+ |
Adgrb3 |
NNNATTTCATCATGAAAAGC |
NGG |
2 |
0.0875 |
Tier II |
| 8 |
NC_000083.6 |
17 |
84746854 |
- |
Lrpprc |
NNNATTTCATCAGGAAAAAC |
NGG |
2 |
0.0584 |
Tier II |
| 9 |
NC_000081.6 |
15 |
27822022 |
+ |
Trio |
NNNATTTCATCATGAAAACC |
NTG |
2 |
0.0273 |
Tier II |
| 10 |
NC_000081.6 |
15 |
47752761 |
+ |
Csmd3 |
NNNATTTCATGACGAAAACC |
NTG |
2 |
0.0097 |
Tier II |
| 11 |
NC_000072.6 |
6 |
52185495 |
+ |
LOC115490409 |
NNNACTTCATCACCAAAACC |
NGG |
2 |
0.4286 |
Tier III |
| 12 |
NC_000070.6 |
4 |
22932471 |
+ |
1700025O08Rik |
NNNATTTCATCATGAAAACT |
NGG |
2 |
0.21 |
Tier III |
Other clones with same target sequence:
(none)
