Construct: sgRNA BRDN0001148628

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGATGCATATCGGAGTTAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK26 (51765)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76001

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 132069535 - STK26 NNNTGCATATCGGAGTTAGG NGG 0 1.0 Tier I
2 NC_000004.12 4 147772957 + ARHGAP10 NNNTACATATAGGAGTTAGG NGG 2 0.65 Tier II
3 NC_000015.10 15 52440826 - MYO5A NNNTGTATATGGGAGTTAGG NGG 2 0.2321 Tier II
4 NC_000019.10 19 29010140 - LINC01532 NNNTGCATATGTGAGTTAGG NGG 2 0.0962 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 16510950 - Csmd1 NNNTGCATATCAGAGTTTGG NGG 2 0.4667 Tier II
2 NC_000072.6 6 36773503 - Ptn NNNTGCATATGGGAGTAAGG NGG 2 0.1333 Tier II
Download CSV

Other clones with same target sequence:

(none)