Construct: sgRNA BRDN0001148638
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGGTTCATATCTTGAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IKBKB (3551)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76091
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
42316726 |
+ |
IKBKB |
NNNGGTTCATATCTTGAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
69587745 |
- |
SULF1 |
NNNGGTTAATAACTTGAACA |
NGG |
2 |
0.52 |
Tier II |
| 3 |
NC_000002.12 |
2 |
53930700 |
+ |
PSME4 |
NNNGGTTAAGATCTTGAACA |
NGG |
2 |
0.325 |
Tier II |
| 4 |
NC_000002.12 |
2 |
211854431 |
- |
ERBB4 |
NNNGTTTTATATCTTGAACA |
NGG |
2 |
0.2625 |
Tier II |
| 5 |
NC_000021.9 |
21 |
32459871 |
- |
EVA1C |
NNNTGTTAATATCTTGAACA |
NGG |
2 |
0.2364 |
Tier II |
| 6 |
NC_000017.11 |
17 |
40750184 |
+ |
KRT25 |
NNNTGTTCATATCTTGAACT |
NGG |
2 |
0.2182 |
Tier II |
| 7 |
NC_000006.12 |
6 |
43013580 |
+ |
MEA1 |
NNNGGTTCTTATCTTGAACC |
NGG |
2 |
0.1364 |
Tier II |
| 8 |
NC_000011.10 |
11 |
99072824 |
- |
CNTN5 |
NNNTGTTCATATCTTGACCA |
NGG |
2 |
0.0693 |
Tier II |
| 9 |
NC_000006.12 |
6 |
71912084 |
+ |
RIMS1 |
NNNGGTTCATATCTTGAACC |
NAG |
2 |
0.0589 |
Tier II |
| 10 |
NC_000012.12 |
12 |
5208026 |
+ |
LOC105369616 |
NNNGCTTGATATCTTGAACA |
NGG |
2 |
0.5051 |
Tier III |
| 11 |
NC_000012.12 |
12 |
5208026 |
+ |
LOC105369617 |
NNNGCTTGATATCTTGAACA |
NGG |
2 |
0.5051 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
22672502 |
- |
Ikbkb |
NNNGGTTCATGTCTTGAACA |
NGG |
1 |
0.65 |
Tier I |
| 2 |
NC_000076.6 |
10 |
64351378 |
- |
Ctnna3 |
NNNGGCTCAAATCTTGAACA |
NGG |
2 |
0.7792 |
Tier II |
| 3 |
NC_000071.6 |
5 |
37639578 |
+ |
Stk32b |
NNNGGACCATATCTTGAACA |
NGG |
2 |
0.5958 |
Tier II |
| 4 |
NC_000075.6 |
9 |
23258862 |
- |
Bmper |
NNNGATTAATATCTTGAACA |
NGG |
2 |
0.5633 |
Tier II |
| 5 |
NC_000072.6 |
6 |
49105143 |
- |
Malsu1 |
NNNGGTCCATGTCTTGAACA |
NGG |
2 |
0.4469 |
Tier II |
| 6 |
NC_000072.6 |
6 |
49105143 |
- |
Igf2bp3 |
NNNGGTCCATGTCTTGAACA |
NGG |
2 |
0.4469 |
Tier II |
| 7 |
NC_000084.6 |
18 |
31220418 |
+ |
Rit2 |
NNNGGTTCATATCAAGAACA |
NGG |
2 |
0.3584 |
Tier II |
| 8 |
NC_000084.6 |
18 |
3316880 |
- |
Crem |
NNNAGTTCATATCTTGAACA |
NAG |
2 |
0.2333 |
Tier II |
| 9 |
NC_000080.6 |
14 |
93028201 |
+ |
Pcdh9 |
NNNGGTTCATATATTGATCA |
NGG |
2 |
0.1923 |
Tier II |
| 10 |
NC_000071.6 |
5 |
20538966 |
+ |
Magi2 |
NNNTGTTCAGATCTTGAACA |
NGG |
2 |
0.1818 |
Tier II |
| 11 |
NC_000067.6 |
1 |
16598369 |
+ |
Ube2w |
NNNGGTTCATATATTGAACA |
NAG |
2 |
0.0997 |
Tier II |
| 12 |
NC_000073.6 |
7 |
25003134 |
- |
Atp1a3 |
NNNGGTTCATATCTTGTCCA |
NGG |
2 |
0.0254 |
Tier II |
| 13 |
NC_000072.6 |
6 |
108507868 |
+ |
Itpr1 |
NNNAGTTCATATCTTGAACA |
NGC |
2 |
0.02 |
Tier II |
| 14 |
NC_000071.6 |
5 |
20538966 |
+ |
Gm52764 |
NNNTGTTCAGATCTTGAACA |
NGG |
2 |
0.1818 |
Tier III |
Other clones with same target sequence:
(none)
