Construct: sgRNA BRDN0001148650
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGAGGTATATTCCCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SCYL1 (57410)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76064
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
65525964 |
- |
SCYL1 |
NNNGAGGTATATTCCCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
94816780 |
+ |
AOPEP |
NNNGAGGTATATTCCCAAAA |
NGG |
2 |
0.4018 |
Tier II |
| 3 |
NC_000006.12 |
6 |
5247164 |
- |
LYRM4 |
NNNGAGGTAAATTCCCAATG |
NGG |
2 |
0.3956 |
Tier II |
| 4 |
NC_000020.11 |
20 |
43030536 |
- |
PTPRT |
NNNGAGGTATATTCACAACA |
NGG |
2 |
0.2083 |
Tier II |
| 5 |
NC_000023.11 |
X |
36131648 |
+ |
CFAP47 |
NNNGTGGTATATTCCCAAAG |
NGG |
2 |
0.1558 |
Tier II |
| 6 |
NC_000018.10 |
18 |
69610351 |
- |
DOK6 |
NNNGAGATATATTCCCAAGG |
NGG |
2 |
0.125 |
Tier II |
| 7 |
NC_000003.12 |
3 |
62602109 |
- |
CADPS |
NNNGAGGTATATTCCCTATG |
NGG |
2 |
0.0615 |
Tier II |
| 8 |
NC_000006.12 |
6 |
109372980 |
- |
CD164 |
NNNGAGGTATATTCCCTAAG |
NGG |
2 |
0.0571 |
Tier II |
| 9 |
NC_000004.12 |
4 |
152795536 |
+ |
ARFIP1 |
NNNGAGGTATATTCCCAGGG |
NGG |
2 |
0.05 |
Tier II |
| 10 |
NC_000001.11 |
1 |
166897692 |
- |
LOC124904450 |
NNNGATGTATATTACCAACG |
NGG |
2 |
0.2333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
88587461 |
- |
Nkd1 |
NNNGAGGCATGTTCCCAACG |
NGG |
2 |
0.65 |
Tier II |
| 2 |
NC_000068.7 |
2 |
134994525 |
- |
Plcb1 |
NNNGATGTATATTCCCAACT |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000081.6 |
15 |
34390637 |
+ |
Matn2 |
NNNGAAGTATATTCCCAACG |
NTG |
2 |
0.039 |
Tier II |
| 4 |
NC_000073.6 |
7 |
118873426 |
- |
Iqck |
NNNGAGGTATATTCCCAAGG |
NGA |
2 |
0.0087 |
Tier II |
Other clones with same target sequence:
(none)
