Construct: sgRNA BRDN0001148669
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCCTGAATCAACATAAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK36 (27148)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77277
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
218693802 |
- |
STK36 |
NNNCTGAATCAACATAAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
188198679 |
- |
LPP |
NNNATGAATCAACATAAACA |
NGG |
1 |
0.8421 |
Tier II |
| 3 |
NC_000006.12 |
6 |
13066766 |
- |
PHACTR1 |
NNNCTGAGACAACATAAACA |
NGG |
2 |
0.681 |
Tier II |
| 4 |
NC_000006.12 |
6 |
166557338 |
+ |
RPS6KA2 |
NNNCTAAATCGACATAAACA |
NGG |
2 |
0.65 |
Tier II |
| 5 |
NC_000004.12 |
4 |
71336761 |
- |
SLC4A4 |
NNNATTAATCAACATAAACA |
NGG |
2 |
0.5614 |
Tier II |
| 6 |
NC_000002.12 |
2 |
153734470 |
+ |
GALNT13 |
NNNCTGAAGAAACATAAACA |
NGG |
2 |
0.5365 |
Tier II |
| 7 |
NC_000002.12 |
2 |
241489118 |
- |
FARP2 |
NNNCTGAATCAACATAAGCA |
NGG |
1 |
0.4 |
Tier II |
| 8 |
NC_000002.12 |
2 |
105833860 |
- |
NCK2 |
NNNCTGAAACAACATAAAAA |
NGG |
2 |
0.398 |
Tier II |
| 9 |
NC_000006.12 |
6 |
152619332 |
+ |
SYNE1 |
NNNCTGATCCAACATAAACA |
NGG |
2 |
0.3956 |
Tier II |
| 10 |
NC_000001.11 |
1 |
207647782 |
- |
CR1L |
NNNCAGAGTCAACATAAACA |
NGG |
2 |
0.3667 |
Tier II |
| 11 |
NC_000013.11 |
13 |
91788766 |
- |
GPC5 |
NNNCTGAAACAAAATAAACA |
NGG |
2 |
0.3571 |
Tier II |
| 12 |
NC_000001.11 |
1 |
65329199 |
- |
DNAJC6 |
NNNCACAATCAACATAAACA |
NGG |
2 |
0.3409 |
Tier II |
| 13 |
NC_000005.10 |
5 |
88185923 |
+ |
TMEM161B |
NNNCTGAATAATCATAAACA |
NGG |
2 |
0.2889 |
Tier II |
| 14 |
NC_000006.12 |
6 |
124668626 |
- |
NKAIN2 |
NNNCTGAATCAACCTAAACA |
NGG |
1 |
0.2143 |
Tier II |
| 15 |
NC_000004.12 |
4 |
16768716 |
- |
LDB2 |
NNNCTGATTCAAAATAAACA |
NGG |
2 |
0.1648 |
Tier II |
| 16 |
NC_000019.10 |
19 |
34432225 |
- |
UBA2 |
NNNCTGAATCATCATAAATA |
NGG |
2 |
0.1538 |
Tier II |
| 17 |
NC_000005.10 |
5 |
90640894 |
- |
ADGRV1 |
NNNCTGAATCACCAAAAACA |
NGG |
2 |
0.1524 |
Tier II |
| 18 |
NC_000015.10 |
15 |
66766599 |
+ |
SMAD6 |
NNNCTGAAGCAACCTAAACA |
NGG |
2 |
0.1327 |
Tier II |
| 19 |
NC_000003.12 |
3 |
23899269 |
+ |
NKIRAS1 |
NNNCTGAATCAACACAAATA |
NGG |
2 |
0.1259 |
Tier II |
| 20 |
NC_000005.10 |
5 |
151846950 |
- |
GLRA1 |
NNNCTGAATCAACAAAGACA |
NGG |
2 |
0.1022 |
Tier II |
| 21 |
NC_000011.10 |
11 |
31731827 |
+ |
ELP4 |
NNNCTGAATCATCACAAACA |
NGG |
2 |
0.0909 |
Tier II |
| 22 |
NC_000006.12 |
6 |
17784583 |
+ |
KIF13A |
NNNCTGAATCAACATAACTA |
NGG |
2 |
0.0879 |
Tier II |
| 23 |
NC_000007.14 |
7 |
130496592 |
- |
MEST |
NNNCTTAATCAACATAAACA |
NGA |
2 |
0.0463 |
Tier II |
| 24 |
NC_000003.12 |
3 |
40232192 |
+ |
MYRIP |
NNNCTGAACCAACAGAAACA |
NGG |
2 |
0.0462 |
Tier II |
| 25 |
NC_000005.10 |
5 |
75410891 |
+ |
CERT1 |
NNNCTGAATTAACATAAACA |
NTG |
2 |
0.0367 |
Tier II |
| 26 |
NC_000011.10 |
11 |
22665136 |
+ |
GAS2 |
NNNCTGAATCAACAAAAACA |
NTG |
2 |
0.0226 |
Tier II |
| 27 |
NC_000009.12 |
9 |
33148450 |
+ |
B4GALT1 |
NNNCTGAATCAACATAAACA |
NGC |
1 |
0.0222 |
Tier II |
| 28 |
NC_000006.12 |
6 |
61859717 |
+ |
KHDRBS2 |
NNNATGAATCAACATAAACA |
NGC |
2 |
0.0187 |
Tier II |
| 29 |
NC_000023.11 |
X |
31925049 |
+ |
DMD |
NNNCTGAAACAACATAAACA |
NGT |
2 |
0.015 |
Tier II |
| 30 |
NC_000001.11 |
1 |
30932198 |
- |
PUM1 |
NNNTTGAATCAACATAAACA |
NGT |
2 |
0.0129 |
Tier II |
| 31 |
NC_000015.10 |
15 |
48419874 |
+ |
FBN1 |
NNNCTGAATGAACATAAACA |
NGT |
2 |
0.0063 |
Tier II |
| 32 |
NC_000011.10 |
11 |
67176333 |
- |
KDM2A |
NNNCTGAATCTACATAAACA |
NGT |
2 |
0.005 |
Tier II |
| 33 |
NC_000012.12 |
12 |
77854273 |
- |
NAV3 |
NNNCTGAATCAACAGAAACA |
NGC |
2 |
0.0011 |
Tier II |
| 34 |
NC_000002.12 |
2 |
11692476 |
- |
LPIN1 |
NNNCTGAGTCAACATCAACA |
NGG |
2 |
0.0 |
Tier II |
| 35 |
NC_000001.11 |
1 |
207647782 |
- |
CD46P1 |
NNNCAGAGTCAACATAAACA |
NGG |
2 |
0.3667 |
Tier III |
| 36 |
NC_000002.12 |
2 |
144559760 |
- |
LINC01412 |
NNNCTGAAACAACATAGACA |
NGG |
2 |
0.1639 |
Tier III |
| 37 |
NC_000012.12 |
12 |
89527271 |
+ |
POC1B-AS1 |
NNNCTGAATCAACATATACA |
NGG |
1 |
0.1333 |
Tier III |
| 38 |
NC_000003.12 |
3 |
40232192 |
+ |
EIF1B-AS1 |
NNNCTGAACCAACAGAAACA |
NGG |
2 |
0.0462 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
125788053 |
- |
Ano2 |
NNNCTGAAAAAACATAAACA |
NGG |
2 |
0.8048 |
Tier II |
| 2 |
NC_000085.6 |
19 |
17342778 |
- |
Gcnt1 |
NNNCTGAATAAACATAAACT |
NGG |
2 |
0.52 |
Tier II |
| 3 |
NC_000068.7 |
2 |
140497196 |
- |
Macrod2 |
NNNCAGAAACAACATAAACA |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000068.7 |
2 |
34457273 |
- |
Mapkap1 |
NNNCTGAATCAACAAAAACG |
NGG |
2 |
0.4427 |
Tier II |
| 5 |
NC_000077.6 |
11 |
47949742 |
- |
Sgcd |
NNNCTGAATCCACATAAACA |
NGG |
1 |
0.4 |
Tier II |
| 6 |
NC_000070.6 |
4 |
14613244 |
- |
Slc26a7 |
NNNCTGAATGAACATAAACA |
NGG |
1 |
0.3889 |
Tier II |
| 7 |
NC_000086.7 |
X |
112115764 |
+ |
Tex16 |
NNNCTGAATGAACATAAACA |
NGG |
1 |
0.3889 |
Tier II |
| 8 |
NC_000070.6 |
4 |
65772914 |
+ |
Astn2 |
NNNCTGAAGCAACAAAAACA |
NGG |
2 |
0.3584 |
Tier II |
| 9 |
NC_000076.6 |
10 |
44322040 |
- |
Atg5 |
NNNCTGAAACAAAATAAACA |
NGG |
2 |
0.3571 |
Tier II |
| 10 |
NC_000068.7 |
2 |
3635590 |
- |
LOC108168726 |
NNNGTGAATCAACAAAAACA |
NGG |
2 |
0.2895 |
Tier II |
| 11 |
NC_000074.6 |
8 |
58461604 |
+ |
Galntl6 |
NNNCTGAATCAACATAAACA |
NAG |
1 |
0.2593 |
Tier II |
| 12 |
NC_000076.6 |
10 |
75170056 |
+ |
Bcr |
NNNCTGACTCAACAAAAACA |
NGG |
2 |
0.2481 |
Tier II |
| 13 |
NC_000077.6 |
11 |
8499285 |
- |
Tns3 |
NNNCTGTATCAAAATAAACA |
NGG |
2 |
0.1683 |
Tier II |
| 14 |
NC_000074.6 |
8 |
124642950 |
- |
2310022B05Rik |
NNNCTGAACCAACATAGACA |
NGG |
2 |
0.1629 |
Tier II |
| 15 |
NC_000071.6 |
5 |
5665831 |
- |
Steap2 |
NNNCTGCATCATCATAAACA |
NGG |
2 |
0.1458 |
Tier II |
| 16 |
NC_000072.6 |
6 |
100759101 |
- |
Gxylt2 |
NNNCTGAATCAACCAAAACA |
NGG |
2 |
0.1241 |
Tier II |
| 17 |
NC_000068.7 |
2 |
77802224 |
- |
Zfp385b |
NNNCTGAAGCAACATACACA |
NGG |
2 |
0.1092 |
Tier II |
| 18 |
NC_000072.6 |
6 |
51927593 |
- |
Skap2 |
NNNCTGAATGAACACAAACA |
NGG |
2 |
0.1061 |
Tier II |
| 19 |
NC_000072.6 |
6 |
142107718 |
- |
Slco1a6 |
NNNCTGAATGAACACAAACA |
NGG |
2 |
0.1061 |
Tier II |
| 20 |
NC_000068.7 |
2 |
79382463 |
- |
Cerkl |
NNNCTGAATCAACTTGAACA |
NGG |
2 |
0.1026 |
Tier II |
| 21 |
NC_000078.6 |
12 |
75148516 |
- |
Kcnh5 |
NNNCTGAATCAAAATAAACA |
NAG |
2 |
0.0997 |
Tier II |
| 22 |
NC_000084.6 |
18 |
76968099 |
- |
Hdhd2 |
NNNCTGAATCAACCTAAAAA |
NGG |
2 |
0.0918 |
Tier II |
| 23 |
NC_000079.6 |
13 |
49586965 |
+ |
Omd |
NNNCTGAATGAACATAAACC |
NGG |
2 |
0.0884 |
Tier II |
| 24 |
NC_000079.6 |
13 |
49586965 |
+ |
Cenpp |
NNNCTGAATGAACATAAACC |
NGG |
2 |
0.0884 |
Tier II |
| 25 |
NC_000081.6 |
15 |
34952429 |
- |
Stk3 |
NNNCTGAATGAACATAAACC |
NGG |
2 |
0.0884 |
Tier II |
| 26 |
NC_000072.6 |
6 |
31444627 |
- |
Mkln1 |
NNNCAGAATCAACATAGACA |
NGG |
2 |
0.0882 |
Tier II |
| 27 |
NC_000086.7 |
X |
109447327 |
+ |
Gm34521 |
NNNCTGAATGAACATAGACA |
NGG |
2 |
0.0686 |
Tier II |
| 28 |
NC_000067.6 |
1 |
138614955 |
+ |
Nek7 |
NNNCTGAATCAAGGTAAACA |
NGG |
2 |
0.0636 |
Tier II |
| 29 |
NC_000077.6 |
11 |
59666129 |
+ |
Mprip |
NNNCTGAATCCACATAAAGA |
NGG |
2 |
0.05 |
Tier II |
| 30 |
NC_000084.6 |
18 |
12891122 |
+ |
Osbpl1a |
NNNCTGAAACAACATAAACA |
NTG |
2 |
0.0362 |
Tier II |
| 31 |
NC_000076.6 |
10 |
31567501 |
- |
Rnf217 |
NNNCTGAATCAAGATAAACA |
NAG |
2 |
0.0354 |
Tier II |
| 32 |
NC_000076.6 |
10 |
53687428 |
- |
Fam184a |
NNNCTGAATCAACATAATCA |
NTG |
2 |
0.0195 |
Tier II |
| 33 |
NC_000084.6 |
18 |
12606454 |
- |
Ttc39c |
NNNCTGAGTCAACATAAACA |
NGT |
2 |
0.0118 |
Tier II |
| 34 |
NC_000068.7 |
2 |
104275645 |
+ |
D430041D05Rik |
NNNCTGAATCAACAGAAACA |
NCG |
2 |
0.0054 |
Tier II |
| 35 |
NC_000085.6 |
19 |
25553787 |
- |
Dmrt1 |
NNNCTGAATCAAGATAAACA |
NGC |
2 |
0.003 |
Tier II |
| 36 |
NC_000084.6 |
18 |
53289228 |
- |
Snx24 |
NNNCTGAATCAACAGAAACA |
NTG |
2 |
0.0019 |
Tier II |
| 37 |
NC_000067.6 |
1 |
38831732 |
+ |
Lonrf2 |
NNNCTGAATAAACATTAACA |
NGG |
2 |
0.0 |
Tier II |
| 38 |
NC_000073.6 |
7 |
100918787 |
+ |
Arhgef17 |
NNNCTGAATCAAAATCAACA |
NGG |
2 |
0.0 |
Tier II |
| 39 |
NC_000072.6 |
6 |
125788053 |
- |
Gm38898 |
NNNCTGAAAAAACATAAACA |
NGG |
2 |
0.8048 |
Tier III |
| 40 |
NC_000072.6 |
6 |
58549562 |
- |
Gm45943 |
NNNGTGAAACAACATAAACA |
NGG |
2 |
0.4643 |
Tier III |
| 41 |
NC_000072.6 |
6 |
67701099 |
+ |
Igk |
NNNGTGAAACAACATAAACA |
NGG |
2 |
0.4643 |
Tier III |
| 42 |
NC_000071.6 |
5 |
5665831 |
- |
Gm40262 |
NNNCTGCATCATCATAAACA |
NGG |
2 |
0.1458 |
Tier III |
| 43 |
NC_000074.6 |
8 |
25271225 |
- |
Gm39145 |
NNNCTTAATCAACATACACA |
NGG |
2 |
0.1176 |
Tier III |
| 44 |
NC_000070.6 |
4 |
87626858 |
- |
Gm34180 |
NNNCTGAATCAACATAAACG |
NTG |
2 |
0.0298 |
Tier III |
| 45 |
NC_000067.6 |
1 |
193684004 |
- |
Gm31870 |
NNNCTTAATCAACATAAACA |
NGC |
2 |
0.0148 |
Tier III |
Other clones with same target sequence:
(none)
