Construct: sgRNA BRDN0001148669
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCCTGAATCAACATAAACA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- STK36 (27148)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000002.12 | 2 | 218693802 | - | STK36 | NNNCTGAATCAACATAAACA | NGG | 0 | 1.0 | Tier I |
| 2 | NC_000003.12 | 3 | 188198679 | - | LPP | NNNATGAATCAACATAAACA | NGG | 1 | 0.8421 | Tier II |
| 3 | NC_000006.12 | 6 | 13066766 | - | PHACTR1 | NNNCTGAGACAACATAAACA | NGG | 2 | 0.681 | Tier II |
| 4 | NC_000006.12 | 6 | 166557338 | + | RPS6KA2 | NNNCTAAATCGACATAAACA | NGG | 2 | 0.65 | Tier II |
| 5 | NC_000004.12 | 4 | 71336761 | - | SLC4A4 | NNNATTAATCAACATAAACA | NGG | 2 | 0.5614 | Tier II |
| 6 | NC_000002.12 | 2 | 153734470 | + | GALNT13 | NNNCTGAAGAAACATAAACA | NGG | 2 | 0.5365 | Tier II |
| 7 | NC_000002.12 | 2 | 241489118 | - | FARP2 | NNNCTGAATCAACATAAGCA | NGG | 1 | 0.4 | Tier II |
| 8 | NC_000002.12 | 2 | 105833860 | - | NCK2 | NNNCTGAAACAACATAAAAA | NGG | 2 | 0.398 | Tier II |
| 9 | NC_000006.12 | 6 | 152619332 | + | SYNE1 | NNNCTGATCCAACATAAACA | NGG | 2 | 0.3956 | Tier II |
| 10 | NC_000001.11 | 1 | 207647782 | - | CR1L | NNNCAGAGTCAACATAAACA | NGG | 2 | 0.3667 | Tier II |
| 11 | NC_000013.11 | 13 | 91788766 | - | GPC5 | NNNCTGAAACAAAATAAACA | NGG | 2 | 0.3571 | Tier II |
| 12 | NC_000001.11 | 1 | 65329199 | - | DNAJC6 | NNNCACAATCAACATAAACA | NGG | 2 | 0.3409 | Tier II |
| 13 | NC_000005.10 | 5 | 88185923 | + | TMEM161B | NNNCTGAATAATCATAAACA | NGG | 2 | 0.2889 | Tier II |
| 14 | NC_000006.12 | 6 | 124668626 | - | NKAIN2 | NNNCTGAATCAACCTAAACA | NGG | 1 | 0.2143 | Tier II |
| 15 | NC_000004.12 | 4 | 16768716 | - | LDB2 | NNNCTGATTCAAAATAAACA | NGG | 2 | 0.1648 | Tier II |
| 16 | NC_000019.10 | 19 | 34432225 | - | UBA2 | NNNCTGAATCATCATAAATA | NGG | 2 | 0.1538 | Tier II |
| 17 | NC_000005.10 | 5 | 90640894 | - | ADGRV1 | NNNCTGAATCACCAAAAACA | NGG | 2 | 0.1524 | Tier II |
| 18 | NC_000015.10 | 15 | 66766599 | + | SMAD6 | NNNCTGAAGCAACCTAAACA | NGG | 2 | 0.1327 | Tier II |
| 19 | NC_000003.12 | 3 | 23899269 | + | NKIRAS1 | NNNCTGAATCAACACAAATA | NGG | 2 | 0.1259 | Tier II |
| 20 | NC_000005.10 | 5 | 151846950 | - | GLRA1 | NNNCTGAATCAACAAAGACA | NGG | 2 | 0.1022 | Tier II |
| 21 | NC_000011.10 | 11 | 31731827 | + | ELP4 | NNNCTGAATCATCACAAACA | NGG | 2 | 0.0909 | Tier II |
| 22 | NC_000006.12 | 6 | 17784583 | + | KIF13A | NNNCTGAATCAACATAACTA | NGG | 2 | 0.0879 | Tier II |
| 23 | NC_000007.14 | 7 | 130496592 | - | MEST | NNNCTTAATCAACATAAACA | NGA | 2 | 0.0463 | Tier II |
| 24 | NC_000003.12 | 3 | 40232192 | + | MYRIP | NNNCTGAACCAACAGAAACA | NGG | 2 | 0.0462 | Tier II |
| 25 | NC_000005.10 | 5 | 75410891 | + | CERT1 | NNNCTGAATTAACATAAACA | NTG | 2 | 0.0367 | Tier II |
| 26 | NC_000011.10 | 11 | 22665136 | + | GAS2 | NNNCTGAATCAACAAAAACA | NTG | 2 | 0.0226 | Tier II |
| 27 | NC_000009.12 | 9 | 33148450 | + | B4GALT1 | NNNCTGAATCAACATAAACA | NGC | 1 | 0.0222 | Tier II |
| 28 | NC_000006.12 | 6 | 61859717 | + | KHDRBS2 | NNNATGAATCAACATAAACA | NGC | 2 | 0.0187 | Tier II |
| 29 | NC_000023.11 | X | 31925049 | + | DMD | NNNCTGAAACAACATAAACA | NGT | 2 | 0.015 | Tier II |
| 30 | NC_000001.11 | 1 | 30932198 | - | PUM1 | NNNTTGAATCAACATAAACA | NGT | 2 | 0.0129 | Tier II |
| 31 | NC_000015.10 | 15 | 48419874 | + | FBN1 | NNNCTGAATGAACATAAACA | NGT | 2 | 0.0063 | Tier II |
| 32 | NC_000011.10 | 11 | 67176333 | - | KDM2A | NNNCTGAATCTACATAAACA | NGT | 2 | 0.005 | Tier II |
| 33 | NC_000012.12 | 12 | 77854273 | - | NAV3 | NNNCTGAATCAACAGAAACA | NGC | 2 | 0.0011 | Tier II |
| 34 | NC_000002.12 | 2 | 11692476 | - | LPIN1 | NNNCTGAGTCAACATCAACA | NGG | 2 | 0.0 | Tier II |
| 35 | NC_000001.11 | 1 | 207647782 | - | CD46P1 | NNNCAGAGTCAACATAAACA | NGG | 2 | 0.3667 | Tier III |
| 36 | NC_000002.12 | 2 | 144559760 | - | LINC01412 | NNNCTGAAACAACATAGACA | NGG | 2 | 0.1639 | Tier III |
| 37 | NC_000012.12 | 12 | 89527271 | + | POC1B-AS1 | NNNCTGAATCAACATATACA | NGG | 1 | 0.1333 | Tier III |
| 38 | NC_000011.10 | 11 | 31731827 | + | ELP4-AS1 | NNNCTGAATCATCACAAACA | NGG | 2 | 0.0909 | Tier III |
| 39 | NC_000003.12 | 3 | 40232192 | + | EIF1B-AS1 | NNNCTGAACCAACAGAAACA | NGG | 2 | 0.0462 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000072.6 | 6 | 125788053 | - | Ano2 | NNNCTGAAAAAACATAAACA | NGG | 2 | 0.8048 | Tier II |
| 2 | NC_000085.6 | 19 | 17342778 | - | Gcnt1 | NNNCTGAATAAACATAAACT | NGG | 2 | 0.52 | Tier II |
| 3 | NC_000068.7 | 2 | 140497196 | - | Macrod2 | NNNCAGAAACAACATAAACA | NGG | 2 | 0.4643 | Tier II |
| 4 | NC_000068.7 | 2 | 34457273 | - | Mapkap1 | NNNCTGAATCAACAAAAACG | NGG | 2 | 0.4427 | Tier II |
| 5 | NC_000077.6 | 11 | 47949742 | - | Sgcd | NNNCTGAATCCACATAAACA | NGG | 1 | 0.4 | Tier II |
| 6 | NC_000070.6 | 4 | 14613244 | - | Slc26a7 | NNNCTGAATGAACATAAACA | NGG | 1 | 0.3889 | Tier II |
| 7 | NC_000086.7 | X | 112115764 | + | Tex16 | NNNCTGAATGAACATAAACA | NGG | 1 | 0.3889 | Tier II |
| 8 | NC_000070.6 | 4 | 65772914 | + | Astn2 | NNNCTGAAGCAACAAAAACA | NGG | 2 | 0.3584 | Tier II |
| 9 | NC_000076.6 | 10 | 44322040 | - | Atg5 | NNNCTGAAACAAAATAAACA | NGG | 2 | 0.3571 | Tier II |
| 10 | NC_000068.7 | 2 | 3635590 | - | LOC108168726 | NNNGTGAATCAACAAAAACA | NGG | 2 | 0.2895 | Tier II |
| 11 | NC_000074.6 | 8 | 58461604 | + | Galntl6 | NNNCTGAATCAACATAAACA | NAG | 1 | 0.2593 | Tier II |
| 12 | NC_000076.6 | 10 | 75170056 | + | Bcr | NNNCTGACTCAACAAAAACA | NGG | 2 | 0.2481 | Tier II |
| 13 | NC_000077.6 | 11 | 8499285 | - | Tns3 | NNNCTGTATCAAAATAAACA | NGG | 2 | 0.1683 | Tier II |
| 14 | NC_000074.6 | 8 | 124642950 | - | 2310022B05Rik | NNNCTGAACCAACATAGACA | NGG | 2 | 0.1629 | Tier II |
| 15 | NC_000071.6 | 5 | 5665831 | - | Steap2 | NNNCTGCATCATCATAAACA | NGG | 2 | 0.1458 | Tier II |
| 16 | NC_000072.6 | 6 | 100759101 | - | Gxylt2 | NNNCTGAATCAACCAAAACA | NGG | 2 | 0.1241 | Tier II |
| 17 | NC_000068.7 | 2 | 77802224 | - | Zfp385b | NNNCTGAAGCAACATACACA | NGG | 2 | 0.1092 | Tier II |
| 18 | NC_000072.6 | 6 | 51927593 | - | Skap2 | NNNCTGAATGAACACAAACA | NGG | 2 | 0.1061 | Tier II |
| 19 | NC_000072.6 | 6 | 142107718 | - | Slco1a6 | NNNCTGAATGAACACAAACA | NGG | 2 | 0.1061 | Tier II |
| 20 | NC_000068.7 | 2 | 79382463 | - | Cerkl | NNNCTGAATCAACTTGAACA | NGG | 2 | 0.1026 | Tier II |
| 21 | NC_000078.6 | 12 | 75148516 | - | Kcnh5 | NNNCTGAATCAAAATAAACA | NAG | 2 | 0.0997 | Tier II |
| 22 | NC_000084.6 | 18 | 76968099 | - | Hdhd2 | NNNCTGAATCAACCTAAAAA | NGG | 2 | 0.0918 | Tier II |
| 23 | NC_000079.6 | 13 | 49586965 | + | Omd | NNNCTGAATGAACATAAACC | NGG | 2 | 0.0884 | Tier II |
| 24 | NC_000079.6 | 13 | 49586965 | + | Cenpp | NNNCTGAATGAACATAAACC | NGG | 2 | 0.0884 | Tier II |
| 25 | NC_000081.6 | 15 | 34952429 | - | Stk3 | NNNCTGAATGAACATAAACC | NGG | 2 | 0.0884 | Tier II |
| 26 | NC_000072.6 | 6 | 31444627 | - | Mkln1 | NNNCAGAATCAACATAGACA | NGG | 2 | 0.0882 | Tier II |
| 27 | NC_000086.7 | X | 109447327 | + | Gm34521 | NNNCTGAATGAACATAGACA | NGG | 2 | 0.0686 | Tier II |
| 28 | NC_000067.6 | 1 | 138614955 | + | Nek7 | NNNCTGAATCAAGGTAAACA | NGG | 2 | 0.0636 | Tier II |
| 29 | NC_000077.6 | 11 | 59666129 | + | Mprip | NNNCTGAATCCACATAAAGA | NGG | 2 | 0.05 | Tier II |
| 30 | NC_000084.6 | 18 | 12891122 | + | Osbpl1a | NNNCTGAAACAACATAAACA | NTG | 2 | 0.0362 | Tier II |
| 31 | NC_000076.6 | 10 | 31567501 | - | Rnf217 | NNNCTGAATCAAGATAAACA | NAG | 2 | 0.0354 | Tier II |
| 32 | NC_000076.6 | 10 | 53687428 | - | Fam184a | NNNCTGAATCAACATAATCA | NTG | 2 | 0.0195 | Tier II |
| 33 | NC_000084.6 | 18 | 12606454 | - | Ttc39c | NNNCTGAGTCAACATAAACA | NGT | 2 | 0.0118 | Tier II |
| 34 | NC_000068.7 | 2 | 104275645 | + | D430041D05Rik | NNNCTGAATCAACAGAAACA | NCG | 2 | 0.0054 | Tier II |
| 35 | NC_000085.6 | 19 | 25553787 | - | Dmrt1 | NNNCTGAATCAAGATAAACA | NGC | 2 | 0.003 | Tier II |
| 36 | NC_000084.6 | 18 | 53289228 | - | Snx24 | NNNCTGAATCAACAGAAACA | NTG | 2 | 0.0019 | Tier II |
| 37 | NC_000067.6 | 1 | 38831732 | + | Lonrf2 | NNNCTGAATAAACATTAACA | NGG | 2 | 0.0 | Tier II |
| 38 | NC_000073.6 | 7 | 100918787 | + | Arhgef17 | NNNCTGAATCAAAATCAACA | NGG | 2 | 0.0 | Tier II |
| 39 | NC_000072.6 | 6 | 125788053 | - | Gm38898 | NNNCTGAAAAAACATAAACA | NGG | 2 | 0.8048 | Tier III |
| 40 | NC_000072.6 | 6 | 58549562 | - | Gm45943 | NNNGTGAAACAACATAAACA | NGG | 2 | 0.4643 | Tier III |
| 41 | NC_000072.6 | 6 | 67701099 | + | Igk | NNNGTGAAACAACATAAACA | NGG | 2 | 0.4643 | Tier III |
| 42 | NC_000071.6 | 5 | 5665831 | - | Gm40262 | NNNCTGCATCATCATAAACA | NGG | 2 | 0.1458 | Tier III |
| 43 | NC_000074.6 | 8 | 25271225 | - | Gm39145 | NNNCTTAATCAACATACACA | NGG | 2 | 0.1176 | Tier III |
| 44 | NC_000070.6 | 4 | 87626858 | - | Gm34180 | NNNCTGAATCAACATAAACG | NTG | 2 | 0.0298 | Tier III |
| 45 | NC_000067.6 | 1 | 193684004 | - | Gm31870 | NNNCTTAATCAACATAAACA | NGC | 2 | 0.0148 | Tier III |