Construct: sgRNA BRDN0001148670
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGGTCTGATTCATAACTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GUCY2C (2984)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77564
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
14688009 |
- |
GUCY2C |
NNNGGTCTGATTCATAACTC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
48955329 |
- |
OTUD5 |
NNNGGTTTGAATCATAACTC |
NGG |
2 |
0.6094 |
Tier II |
| 3 |
NC_000003.12 |
3 |
100385327 |
+ |
TOMM70 |
NNNGGTCTGGTTCAAAACTC |
NGG |
2 |
0.3216 |
Tier II |
| 4 |
NC_000019.10 |
19 |
38389767 |
+ |
SPRED3 |
NNNGGTCTCATTCATAAATC |
NGG |
2 |
0.2899 |
Tier II |
| 5 |
NC_000016.10 |
16 |
3303351 |
- |
TIGD7 |
NNNGGCCTGATTCATAACAC |
NGG |
2 |
0.2597 |
Tier II |
| 6 |
NC_000015.10 |
15 |
34392721 |
+ |
GOLGA8A |
NNNGGTCTGAATCATAACAC |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000015.10 |
15 |
34538942 |
+ |
GOLGA8B |
NNNGGTCTGAATCATAACAC |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000008.11 |
8 |
42890944 |
+ |
RNF170 |
NNNGGTCTGATTTATAACTC |
NAG |
2 |
0.1815 |
Tier II |
| 9 |
NC_000012.12 |
12 |
100234685 |
- |
DEPDC4 |
NNNGGTCTGATTCATAACAC |
NTG |
2 |
0.0111 |
Tier II |
| 10 |
NC_000014.9 |
14 |
72986402 |
- |
ZFYVE1 |
NNNGGTCTGATTCATTAGTC |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000023.11 |
X |
130087802 |
+ |
ELF4 |
NNNGGTCTGATTCGTTACTC |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000012.12 |
12 |
14688009 |
- |
GUCY2C-AS1 |
NNNGGTCTGATTCATAACTC |
NGG |
0 |
1.0 |
Tier III |
| 13 |
NC_000014.9 |
14 |
69190559 |
+ |
GALNT16-AS1 |
NNNGGTTTGATTCCTAACTC |
NGG |
2 |
0.1741 |
Tier III |
| 14 |
NC_000007.14 |
7 |
7887188 |
+ |
LOC124901586 |
NNNTGTCTGATTCATAACTC |
NGA |
2 |
0.0253 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
136773732 |
- |
Gucy2c |
NNNGGTCTGATTCATAAGTC |
NGG |
1 |
0.1333 |
Tier I |
| 2 |
NC_000073.6 |
7 |
29168491 |
- |
Spred3 |
NNNGGTCTCATTCATAAATC |
NGG |
2 |
0.2899 |
Tier II |
| 3 |
NC_000073.6 |
7 |
29168491 |
- |
Ggn |
NNNGGTCTCATTCATAAATC |
NGG |
2 |
0.2899 |
Tier II |
| 4 |
NC_000078.6 |
12 |
81514408 |
+ |
Synj2bp |
NNNGGTTTGATTCATAACTC |
NAG |
2 |
0.2106 |
Tier II |
| 5 |
NC_000078.6 |
12 |
81514408 |
+ |
Gm20498 |
NNNGGTTTGATTCATAACTC |
NAG |
2 |
0.2106 |
Tier II |
| 6 |
NC_000071.6 |
5 |
131783971 |
- |
Auts2 |
NNNGGTGTGATTCACAACTC |
NGG |
2 |
0.1283 |
Tier II |
| 7 |
NC_000069.6 |
3 |
109515710 |
+ |
Vav3 |
NNNTGTCTGATTCATAACCC |
NGG |
2 |
0.1003 |
Tier II |
| 8 |
NC_000083.6 |
17 |
70043664 |
- |
Dlgap1 |
NNNGGTCTGATTCATATCCC |
NGG |
2 |
0.0368 |
Tier II |
| 9 |
NC_000078.6 |
12 |
32760007 |
+ |
Gm40376 |
NNNGGTCAGATGCATAACTC |
NGG |
2 |
0.4 |
Tier III |
| 10 |
NC_000080.6 |
14 |
45405998 |
+ |
Gm41143 |
NNNGGCCTGATTCATAACTC |
NGA |
2 |
0.0631 |
Tier III |
Other clones with same target sequence:
(none)
