Construct: sgRNA BRDN0001148673
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGGATCCTCATCAAGCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERBB2 (2064)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76377
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
39723401 |
+ |
ERBB2 |
NNNGGATCCTCATCAAGCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
26898539 |
+ |
DPYSL5 |
NNNGGATCCTCATCAAGGGA |
NGC |
2 |
0.003 |
Tier I |
| 3 |
NC_000017.11 |
17 |
38781505 |
- |
PIP4K2B |
NNNGGATCCTCATTAAGAGA |
NGG |
2 |
0.3949 |
Tier II |
| 4 |
NC_000016.10 |
16 |
81802534 |
- |
PLCG2 |
NNNGGTTCCTCATCAAGAGA |
NGG |
2 |
0.3846 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
42477159 |
- |
Ptchd4 |
NNNGCATCCTCATCAAGCTA |
NGG |
2 |
0.5238 |
Tier II |
| 2 |
NC_000082.6 |
16 |
25304384 |
+ |
Tprg |
NNNGGATCCCCATCAAGAGA |
NGG |
2 |
0.2872 |
Tier II |
| 3 |
NC_000074.6 |
8 |
105542360 |
- |
Atp6v0d1 |
NNNGGATCCTCCTCAAGCTA |
NGG |
2 |
0.1754 |
Tier II |
| 4 |
NC_000084.6 |
18 |
23885996 |
+ |
Mapre2 |
NNNGGATCATCATCAAGCGA |
NGA |
2 |
0.0595 |
Tier II |
| 5 |
NC_000070.6 |
4 |
104490482 |
- |
Dab1 |
NNNAGATCCTCATGAAGCGA |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000074.6 |
8 |
76879925 |
+ |
Gm10649 |
NNNGGATCCTGGTCAAGCGA |
NGG |
2 |
0.1806 |
Tier III |
Other clones with same target sequence:
(none)
