Construct: sgRNA BRDN0001148678
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGCCCAAAATGGAGTGTCA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- EPHA1 (2041)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000007.14 | 7 | 143398662 | - | EPHA1 | NNNCCCAAAATGGAGTGTCA | NGG | 0 | 1.0 | Tier I |
2 | NC_000012.12 | 12 | 26195927 | - | SSPN | NNNCCCAAAATGGAGTGTCC | NTG | 2 | 0.0089 | Tier I |
3 | NC_000012.12 | 12 | 130624779 | + | RIMBP2 | NNNCCCAGAATGGAGTGTCA | NGG | 1 | 0.7333 | Tier II |
4 | NC_000005.10 | 5 | 108923723 | - | FER | NNNCACAAAATGAAGTGTCA | NGG | 2 | 0.5275 | Tier II |
5 | NC_000009.12 | 9 | 111434953 | + | ECPAS | NNNCCCAGGATGGAGTGTCA | NGG | 2 | 0.4889 | Tier II |
6 | NC_000003.12 | 3 | 96819583 | - | EPHA6 | NNNCCCAAAATGGTGAGTCA | NGG | 2 | 0.4848 | Tier II |
7 | NC_000023.11 | X | 65599597 | - | MSN | NNNCCCACAATGGAGTGTCA | NGG | 1 | 0.4286 | Tier II |
8 | NC_000004.12 | 4 | 109714789 | + | PLA2G12A | NNNCCCAGAGTGGAGTGTCA | NGG | 2 | 0.4074 | Tier II |
9 | NC_000014.9 | 14 | 64868073 | - | SPTB | NNNCCCAAAATGAAGTGTAA | NGG | 2 | 0.3956 | Tier II |
10 | NC_000001.11 | 1 | 97655279 | + | DPYD | NNNCCCACAATGAAGTGTCA | NGG | 2 | 0.3956 | Tier II |
11 | NC_000002.12 | 2 | 70762401 | + | ADD2 | NNNCCCACAATGGAGAGTCA | NGG | 2 | 0.3896 | Tier II |
12 | NC_000004.12 | 4 | 109741641 | - | CFI | NNNCCCAAAAGGGAGTATCA | NGG | 2 | 0.3733 | Tier II |
13 | NC_000008.11 | 8 | 48038372 | + | UBE2V2 | NNNCCCAAAATGGAGTGTAG | NGG | 2 | 0.3277 | Tier II |
14 | NC_000002.12 | 2 | 214405179 | + | SPAG16 | NNNCCCAGAATGGAGTGTAA | NGG | 2 | 0.3143 | Tier II |
15 | NC_000013.11 | 13 | 75572466 | + | UCHL3 | NNNCACAAAATGGTGTGTCA | NGG | 2 | 0.3048 | Tier II |
16 | NC_000007.14 | 7 | 104382028 | - | LHFPL3 | NNNCTCAAAATGGAGTGTTA | NGG | 2 | 0.2937 | Tier II |
17 | NC_000009.12 | 9 | 983830 | + | DMRT3 | NNNCCCAAAATATAGTGTCA | NGG | 2 | 0.28 | Tier II |
18 | NC_000005.10 | 5 | 72492338 | + | ZNF366 | NNNCCCAACATGGAGTGCCA | NGG | 2 | 0.2449 | Tier II |
19 | NC_000017.11 | 17 | 66572431 | - | PRKCA | NNNCCCAGACTGGAGTGTCA | NGG | 2 | 0.2444 | Tier II |
20 | NC_000020.11 | 20 | 25348596 | - | ABHD12 | NNNCCCAGACTGGAGTGTCA | NGG | 2 | 0.2444 | Tier II |
21 | NC_000007.14 | 7 | 138752912 | + | ATP6V0A4 | NNNCCAAAAATGGAGTGTCA | NAG | 2 | 0.2407 | Tier II |
22 | NC_000023.11 | X | 151577281 | - | PASD1 | NNNCCCAAAATGGAATTTCA | NGG | 2 | 0.2353 | Tier II |
23 | NC_000016.10 | 16 | 71093176 | + | HYDIN | NNNCCCAAAATGAAGTTTCA | NGG | 2 | 0.2308 | Tier II |
24 | NC_000001.11 | 1 | 31745583 | + | ADGRB2 | NNNCCCACAATGGGGTGTCA | NGG | 2 | 0.2 | Tier II |
25 | NC_000011.10 | 11 | 12881972 | - | TEAD1 | NNNCCCAAAAAGGAGTTTCA | NGG | 2 | 0.1875 | Tier II |
26 | NC_000007.14 | 7 | 5632915 | + | RNF216 | NNNCCCCAAATGCAGTGTCA | NGG | 2 | 0.1842 | Tier II |
27 | NC_000016.10 | 16 | 78714007 | - | WWOX | NNNCCCCAAATGCAGTGTCA | NGG | 2 | 0.1842 | Tier II |
28 | NC_000017.11 | 17 | 67678781 | - | PITPNC1 | NNNCCCAAAATGGAGTGGAA | NGG | 2 | 0.1429 | Tier II |
29 | NC_000001.11 | 1 | 41598870 | + | HIVEP3 | NNNCCCAAACTGGAGTGTAA | NGG | 2 | 0.1429 | Tier II |
30 | NC_000008.11 | 8 | 24328885 | - | ADAM28 | NNNCCCAAACTGGAGTGTAA | NGG | 2 | 0.1429 | Tier II |
31 | NC_000017.11 | 17 | 40020060 | + | MED24 | NNNCCCAAAATGGGGTGTCA | NAG | 2 | 0.121 | Tier II |
32 | NC_000017.11 | 17 | 64488689 | + | POLG2 | NNNCCCAAAATGAAGTGTGA | NGG | 2 | 0.1154 | Tier II |
33 | NC_000017.11 | 17 | 64488689 | + | MILR1 | NNNCCCAAAATGAAGTGTGA | NGG | 2 | 0.1154 | Tier II |
34 | NC_000018.10 | 18 | 25313863 | - | ZNF521 | NNNCCCAAAATGCAGTTTCA | NGG | 2 | 0.1053 | Tier II |
35 | NC_000001.11 | 1 | 64840499 | - | JAK1 | NNNCCCAAAATAGAGTGTCA | NCG | 2 | 0.1 | Tier II |
36 | NC_000012.12 | 12 | 108807019 | + | SSH1 | NNNCCCAAAATGCAGTGTCC | NGG | 2 | 0.0957 | Tier II |
37 | NC_000001.11 | 1 | 44854642 | + | EIF2B3 | NNNCCCAAGATGGAGTGTGA | NGG | 2 | 0.0833 | Tier II |
38 | NC_000010.11 | 10 | 24659371 | + | ARHGAP21 | NNNCCCAAGATGGAGTGTGA | NGG | 2 | 0.0833 | Tier II |
39 | NC_000010.11 | 10 | 12908181 | + | CCDC3 | NNNCCCAAAATGGAGTCTCC | NGG | 2 | 0.0535 | Tier II |
40 | NC_000007.14 | 7 | 8177019 | + | ICA1 | NNNCCCAAAATGGAGTGTCA | NCA | 2 | 0.0 | Tier II |
41 | NC_000022.11 | 22 | 22145551 | + | IGL | NNNCCCAAATTGGAGTGTAA | NGG | 2 | 0.3782 | Tier III |
42 | NC_000011.10 | 11 | 76306178 | + | LOC105369395 | NNNCCCAAAGTGGAGTGTCT | NGG | 2 | 0.3333 | Tier III |
43 | NC_000001.11 | 1 | 146617783 | - | HYDIN2 | NNNCCCAAAATGAAGTTTCA | NGG | 2 | 0.2308 | Tier III |
44 | NC_000017.11 | 17 | 67678781 | - | LOC101928045 | NNNCCCAAAATGGAGTGGAA | NGG | 2 | 0.1429 | Tier III |
45 | NC_000008.11 | 8 | 24328885 | - | ADAM7-AS1 | NNNCCCAAACTGGAGTGTAA | NGG | 2 | 0.1429 | Tier III |
46 | NC_000016.10 | 16 | 5147538 | + | ENPP7P14 | NNNCCCAAACTGGAGTGTAA | NGG | 2 | 0.1429 | Tier III |
47 | NC_000023.11 | X | 78691817 | + | LOC107985670 | NNNCCCAAACTGGAGTGTAA | NGG | 2 | 0.1429 | Tier III |
48 | NC_000012.12 | 12 | 108807019 | + | LOC101929204 | NNNCCCAAAATGCAGTGTCC | NGG | 2 | 0.0957 | Tier III |
49 | NC_000009.12 | 9 | 22820000 | - | LINC01239 | NNNCCCAAAATGGAGTGGCA | NAG | 2 | 0.0864 | Tier III |
50 | NC_000010.11 | 10 | 12908181 | + | LOC105376418 | NNNCCCAAAATGGAGTCTCC | NGG | 2 | 0.0535 | Tier III |
51 | NC_000001.11 | 1 | 217188470 | + | LOC105372920 | NNNCCCAAAATGTATTGTCA | NGG | 2 | 0.0429 | Tier III |
52 | NC_000009.12 | 9 | 21513446 | + | MIR31HG | NNNCCCAAAATGGAGTGTAA | NGT | 2 | 0.0069 | Tier III |
53 | NC_000002.12 | 2 | 65742784 | - | LOC105369167 | NNNCCCAAAATTGAGTGTCA | NGT | 2 | 0.0062 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000074.6 | 8 | 120571566 | + | Gse1 | NNNCCCAAAATGGGGAGTCA | NGG | 2 | 0.4242 | Tier II |
2 | NC_000083.6 | 17 | 5109293 | + | Arid1b | NNNCCCCAAATGGAATGTCA | NGG | 2 | 0.4118 | Tier II |
3 | NC_000072.6 | 6 | 45965752 | + | Cntnap2 | NNNCCCAAACTGGAGAGTCA | NGG | 2 | 0.303 | Tier II |
4 | NC_000078.6 | 12 | 40586659 | - | Dock4 | NNNCCCATAATGGTGTGTCA | NGG | 2 | 0.2286 | Tier II |
5 | NC_000074.6 | 8 | 95149449 | - | Kifc3 | NNNCCCAGAATGTAGTGTCA | NGG | 2 | 0.22 | Tier II |
6 | NC_000086.7 | X | 162933097 | + | Ctps2 | NNNCCCAAAATGGAGCCTCA | NGG | 2 | 0.1569 | Tier II |
7 | NC_000073.6 | 7 | 118655086 | - | Tmc5 | NNNCCCAAAATGAAGTGTGA | NGG | 2 | 0.1154 | Tier II |
8 | NC_000074.6 | 8 | 111162535 | - | Glg1 | NNNCCCAAAATGGGGTGTGA | NGG | 2 | 0.0583 | Tier II |
9 | NC_000071.6 | 5 | 81504936 | + | Adgrl3 | NNNCCCAAAAAGGAGTGTCA | NGA | 2 | 0.0521 | Tier II |
10 | NC_000071.6 | 5 | 34897726 | - | Htt | NNNCCCAAAATGGAGTGGCA | NCG | 2 | 0.0357 | Tier II |
11 | NC_000072.6 | 6 | 135800625 | - | Grin2b | NNNGCCAAAATGGAGTGTCA | NGA | 2 | 0.0347 | Tier II |
12 | NC_000067.6 | 1 | 9290981 | + | Sntg1 | NNNCCCAAAATGGAGTGTCA | NCA | 2 | 0.0 | Tier II |
13 | NC_000085.6 | 19 | 59781353 | + | Gm46662 | NNNCTCAAAATGGTGTGTCA | NGG | 2 | 0.3394 | Tier III |