Construct: sgRNA BRDN0001148678

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGCCCAAAATGGAGTGTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA1 (2041)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75591

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 143398662 - EPHA1 NNNCCCAAAATGGAGTGTCA NGG 0 1.0 Tier I
2 NC_000012.12 12 26195927 - SSPN NNNCCCAAAATGGAGTGTCC NTG 2 0.0089 Tier I
3 NC_000012.12 12 130624779 + RIMBP2 NNNCCCAGAATGGAGTGTCA NGG 1 0.7333 Tier II
4 NC_000005.10 5 108923723 - FER NNNCACAAAATGAAGTGTCA NGG 2 0.5275 Tier II
5 NC_000009.12 9 111434953 + ECPAS NNNCCCAGGATGGAGTGTCA NGG 2 0.4889 Tier II
6 NC_000003.12 3 96819583 - EPHA6 NNNCCCAAAATGGTGAGTCA NGG 2 0.4848 Tier II
7 NC_000023.11 X 65599597 - MSN NNNCCCACAATGGAGTGTCA NGG 1 0.4286 Tier II
8 NC_000004.12 4 109714789 + PLA2G12A NNNCCCAGAGTGGAGTGTCA NGG 2 0.4074 Tier II
9 NC_000014.9 14 64868073 - SPTB NNNCCCAAAATGAAGTGTAA NGG 2 0.3956 Tier II
10 NC_000001.11 1 97655279 + DPYD NNNCCCACAATGAAGTGTCA NGG 2 0.3956 Tier II
11 NC_000002.12 2 70762401 + ADD2 NNNCCCACAATGGAGAGTCA NGG 2 0.3896 Tier II
12 NC_000004.12 4 109741641 - CFI NNNCCCAAAAGGGAGTATCA NGG 2 0.3733 Tier II
13 NC_000008.11 8 48038372 + UBE2V2 NNNCCCAAAATGGAGTGTAG NGG 2 0.3277 Tier II
14 NC_000002.12 2 214405179 + SPAG16 NNNCCCAGAATGGAGTGTAA NGG 2 0.3143 Tier II
15 NC_000013.11 13 75572466 + UCHL3 NNNCACAAAATGGTGTGTCA NGG 2 0.3048 Tier II
16 NC_000007.14 7 104382028 - LHFPL3 NNNCTCAAAATGGAGTGTTA NGG 2 0.2937 Tier II
17 NC_000009.12 9 983830 + DMRT3 NNNCCCAAAATATAGTGTCA NGG 2 0.28 Tier II
18 NC_000005.10 5 72492338 + ZNF366 NNNCCCAACATGGAGTGCCA NGG 2 0.2449 Tier II
19 NC_000017.11 17 66572431 - PRKCA NNNCCCAGACTGGAGTGTCA NGG 2 0.2444 Tier II
20 NC_000020.11 20 25348596 - ABHD12 NNNCCCAGACTGGAGTGTCA NGG 2 0.2444 Tier II
21 NC_000007.14 7 138752912 + ATP6V0A4 NNNCCAAAAATGGAGTGTCA NAG 2 0.2407 Tier II
22 NC_000023.11 X 151577281 - PASD1 NNNCCCAAAATGGAATTTCA NGG 2 0.2353 Tier II
23 NC_000016.10 16 71093176 + HYDIN NNNCCCAAAATGAAGTTTCA NGG 2 0.2308 Tier II
24 NC_000001.11 1 31745583 + ADGRB2 NNNCCCACAATGGGGTGTCA NGG 2 0.2 Tier II
25 NC_000011.10 11 12881972 - TEAD1 NNNCCCAAAAAGGAGTTTCA NGG 2 0.1875 Tier II
26 NC_000007.14 7 5632915 + RNF216 NNNCCCCAAATGCAGTGTCA NGG 2 0.1842 Tier II
27 NC_000016.10 16 78714007 - WWOX NNNCCCCAAATGCAGTGTCA NGG 2 0.1842 Tier II
28 NC_000017.11 17 67678781 - PITPNC1 NNNCCCAAAATGGAGTGGAA NGG 2 0.1429 Tier II
29 NC_000001.11 1 41598870 + HIVEP3 NNNCCCAAACTGGAGTGTAA NGG 2 0.1429 Tier II
30 NC_000008.11 8 24328885 - ADAM28 NNNCCCAAACTGGAGTGTAA NGG 2 0.1429 Tier II
31 NC_000017.11 17 40020060 + MED24 NNNCCCAAAATGGGGTGTCA NAG 2 0.121 Tier II
32 NC_000017.11 17 64488689 + POLG2 NNNCCCAAAATGAAGTGTGA NGG 2 0.1154 Tier II
33 NC_000017.11 17 64488689 + MILR1 NNNCCCAAAATGAAGTGTGA NGG 2 0.1154 Tier II
34 NC_000018.10 18 25313863 - ZNF521 NNNCCCAAAATGCAGTTTCA NGG 2 0.1053 Tier II
35 NC_000001.11 1 64840499 - JAK1 NNNCCCAAAATAGAGTGTCA NCG 2 0.1 Tier II
36 NC_000012.12 12 108807019 + SSH1 NNNCCCAAAATGCAGTGTCC NGG 2 0.0957 Tier II
37 NC_000001.11 1 44854642 + EIF2B3 NNNCCCAAGATGGAGTGTGA NGG 2 0.0833 Tier II
38 NC_000010.11 10 24659371 + ARHGAP21 NNNCCCAAGATGGAGTGTGA NGG 2 0.0833 Tier II
39 NC_000010.11 10 12908181 + CCDC3 NNNCCCAAAATGGAGTCTCC NGG 2 0.0535 Tier II
40 NC_000007.14 7 8177019 + ICA1 NNNCCCAAAATGGAGTGTCA NCA 2 0.0 Tier II
41 NC_000022.11 22 22145551 + IGL NNNCCCAAATTGGAGTGTAA NGG 2 0.3782 Tier III
42 NC_000011.10 11 76306178 + LOC105369395 NNNCCCAAAGTGGAGTGTCT NGG 2 0.3333 Tier III
43 NC_000001.11 1 146617783 - HYDIN2 NNNCCCAAAATGAAGTTTCA NGG 2 0.2308 Tier III
44 NC_000017.11 17 67678781 - LOC101928045 NNNCCCAAAATGGAGTGGAA NGG 2 0.1429 Tier III
45 NC_000008.11 8 24328885 - ADAM7-AS1 NNNCCCAAACTGGAGTGTAA NGG 2 0.1429 Tier III
46 NC_000016.10 16 5147538 + ENPP7P14 NNNCCCAAACTGGAGTGTAA NGG 2 0.1429 Tier III
47 NC_000023.11 X 78691817 + LOC107985670 NNNCCCAAACTGGAGTGTAA NGG 2 0.1429 Tier III
48 NC_000012.12 12 108807019 + LOC101929204 NNNCCCAAAATGCAGTGTCC NGG 2 0.0957 Tier III
49 NC_000009.12 9 22820000 - LINC01239 NNNCCCAAAATGGAGTGGCA NAG 2 0.0864 Tier III
50 NC_000010.11 10 12908181 + LOC105376418 NNNCCCAAAATGGAGTCTCC NGG 2 0.0535 Tier III
51 NC_000001.11 1 217188470 + LOC105372920 NNNCCCAAAATGTATTGTCA NGG 2 0.0429 Tier III
52 NC_000009.12 9 21513446 + MIR31HG NNNCCCAAAATGGAGTGTAA NGT 2 0.0069 Tier III
53 NC_000002.12 2 65742784 - LOC105369167 NNNCCCAAAATTGAGTGTCA NGT 2 0.0062 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 120571566 + Gse1 NNNCCCAAAATGGGGAGTCA NGG 2 0.4242 Tier II
2 NC_000083.6 17 5109293 + Arid1b NNNCCCCAAATGGAATGTCA NGG 2 0.4118 Tier II
3 NC_000072.6 6 45965752 + Cntnap2 NNNCCCAAACTGGAGAGTCA NGG 2 0.303 Tier II
4 NC_000078.6 12 40586659 - Dock4 NNNCCCATAATGGTGTGTCA NGG 2 0.2286 Tier II
5 NC_000074.6 8 95149449 - Kifc3 NNNCCCAGAATGTAGTGTCA NGG 2 0.22 Tier II
6 NC_000086.7 X 162933097 + Ctps2 NNNCCCAAAATGGAGCCTCA NGG 2 0.1569 Tier II
7 NC_000073.6 7 118655086 - Tmc5 NNNCCCAAAATGAAGTGTGA NGG 2 0.1154 Tier II
8 NC_000074.6 8 111162535 - Glg1 NNNCCCAAAATGGGGTGTGA NGG 2 0.0583 Tier II
9 NC_000071.6 5 81504936 + Adgrl3 NNNCCCAAAAAGGAGTGTCA NGA 2 0.0521 Tier II
10 NC_000071.6 5 34897726 - Htt NNNCCCAAAATGGAGTGGCA NCG 2 0.0357 Tier II
11 NC_000072.6 6 135800625 - Grin2b NNNGCCAAAATGGAGTGTCA NGA 2 0.0347 Tier II
12 NC_000067.6 1 9290981 + Sntg1 NNNCCCAAAATGGAGTGTCA NCA 2 0.0 Tier II
13 NC_000085.6 19 59781353 + Gm46662 NNNCTCAAAATGGTGTGTCA NGG 2 0.3394 Tier III
Download CSV

Other clones with same target sequence:

(none)