Construct: sgRNA BRDN0001148684

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAGATCACCAGCCAAACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FES (2242)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76506

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 90885437 + FES NNNGATCACCAGCCAAACTG NGG 0 1.0 Tier I
2 NC_000018.10 18 21450023 - GREB1L NNNGGGCACCAGCCAAACTG NGG 2 0.4114 Tier II
3 NC_000008.11 8 140425659 + TRAPPC9 NNNCATAACCAGCCAAACTG NGG 2 0.3971 Tier II
4 NC_000002.12 2 233934780 - TRPM8 NNNGATTCCCAGCCAAACTG NGG 2 0.3482 Tier II
5 NC_000017.11 17 49819190 - KAT7 NNNTATCACCAACCAAACTG NGG 2 0.3394 Tier II
6 NC_000008.11 8 3957906 - CSMD1 NNNGATCACCAGCCAAACAA NGG 2 0.2679 Tier II
7 NC_000018.10 18 3414500 - TGIF1 NNNGATCACCAACCCAACTG NGG 2 0.2545 Tier II
8 NC_000014.9 14 29686231 - PRKD1 NNNTATCACCAGCCAAATTG NGG 2 0.2338 Tier II
9 NC_000006.12 6 1711896 + GMDS NNNGAGCACCAGCCAAACGG NGG 2 0.1429 Tier II
10 NC_000012.12 12 9128305 + KLRG1 NNNGGTCACCAGCCAGACTG NGG 2 0.1385 Tier II
11 NC_000015.10 15 74821943 - LMAN1L NNNGAGCACCAGCCAAGCTG NGG 2 0.1008 Tier II
12 NC_000016.10 16 78579414 + WWOX NNNGTTCACCAGGCAAACTG NGG 2 0.0496 Tier II
13 NC_000008.11 8 62721521 + NKAIN3 NNNGAACACCAGCCAAACTG NGC 2 0.0193 Tier II
14 NC_000004.12 4 8616538 - CPZ NNNGATGACCAGCCACACTG NGG 2 0.0 Tier II
15 NC_000005.10 5 69545385 - OCLN NNNGTTCACCAGCCACACTG NGG 2 0.0 Tier II
16 NC_000021.9 21 35396166 + LOC100506403 NNNGAACACCAGCTAAACTG NGG 2 0.6356 Tier III
17 NC_000018.10 18 21450023 - LOC101927521 NNNGGGCACCAGCCAAACTG NGG 2 0.4114 Tier III
18 NC_000007.14 7 143256477 + TMEM139-AS1 NNNGAGCCCCAGCCAAACTG NGG 2 0.2449 Tier III
19 NC_000007.14 7 143256477 + LOC105375546 NNNGAGCCCCAGCCAAACTG NGG 2 0.2449 Tier III
20 NC_000012.12 12 9128305 + KRT17P8 NNNGGTCACCAGCCAGACTG NGG 2 0.1385 Tier III
21 NC_000006.12 6 129922570 + LOC105377999 NNNGATCACCAGCCAATCTG NGG 1 0.1333 Tier III
22 NC_000003.12 3 142990531 - PAQR9-AS1 NNNGATCACCAGGCAAACTT NGG 2 0.0955 Tier III
23 NC_000001.11 1 175916684 - LINC02803 NNNGATCACCAGCAAATCTG NGG 2 0.0467 Tier III
24 NC_000004.12 4 8616538 - LOC124900659 NNNGATGACCAGCCACACTG NGG 2 0.0 Tier III
25 NC_000005.10 5 71082512 + OCLNP1 NNNGTTCACCAGCCACACTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 34174405 - Kalrn NNNAAACACCAGCCAAACTG NGG 2 0.78 Tier I
2 NC_000070.6 4 40260375 - Topors NNNGAGCACCAGCCAAACTG NGG 1 0.5714 Tier I
3 NC_000071.6 5 30872687 - Tmem214 NNNGATCCCCAGCCACACTG NGG 2 0.0 Tier I
4 NC_000076.6 10 20501403 - Pde7b NNNAATCACCACCCAAACTG NGG 2 0.4765 Tier II
5 NC_000075.6 9 75572885 - Tmod2 NNNGATCACCAGCTAAATTG NGG 2 0.4714 Tier II
6 NC_000071.6 5 34849361 + Htt NNNGATCATCAGCCAAACAG NGG 2 0.25 Tier II
7 NC_000074.6 8 88260995 - Tent4b NNNGCTCACCAGCCAAACTC NGG 2 0.2143 Tier II
8 NC_000073.6 7 65510992 + Tjp1 NNNGATCATCAGCCAGACTG NGG 2 0.1683 Tier II
9 NC_000086.7 X 159861487 - Sh3kbp1 NNNGATCACAAGGCAAACTG NGG 2 0.1182 Tier II
10 NC_000070.6 4 155502300 - Gnb1 NNNGATCACCCGCCAACCTG NGG 2 0.0706 Tier II
11 NC_000080.6 14 54898559 - Pabpn1 NNNGATCACCAGGCAAACTG NAG 2 0.0354 Tier II
12 NC_000071.6 5 108684380 + Idua NNNGATCACCAGCCAAAGGG NGG 2 0.0333 Tier II
13 NC_000077.6 11 46110116 + Adam19 NNNGATCACCAGCCAGTCTG NGG 2 0.0256 Tier II
14 NC_000076.6 10 92786019 - Cfap54 NNNGATCACCAGCCATCCTG NGG 2 0.0 Tier II
15 NC_000068.7 2 33574942 - Lmx1b NNNGATCACCTGCCACACTG NGG 2 0.0 Tier II
16 NC_000069.6 3 36404028 - Gm42209 NNNGATGACCAGCCAAACAG NGG 2 0.1345 Tier III
17 NC_000079.6 13 72546228 + Gm38580 NNNGATCAGCAGCCAAAGTG NGG 2 0.0825 Tier III
18 NC_000070.6 4 155502300 - Gm13171 NNNGATCACCCGCCAACCTG NGG 2 0.0706 Tier III
19 NC_000071.6 5 108684380 + Gm42151 NNNGATCACCAGCCAAAGGG NGG 2 0.0333 Tier III
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Other clones with same target sequence:

(none)