Construct: sgRNA BRDN0001148687

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAAACCAAGGATGATCTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HIPK2 (28996)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76749

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 139631718 + HIPK2 NNNACCAAGGATGATCTCAG NGG 0 1.0 Tier I
2 NC_000001.11 1 226302684 + LIN9 NNNATTAAGGATGATCTCAG NGG 2 0.5909 Tier II
3 NC_000013.11 13 93446364 - GPC6 NNNAACAAAGATGATCTCAG NGG 2 0.3673 Tier II
4 NC_000003.12 3 49866874 - CAMKV NNNAACCAGGATGATCTCAG NGG 2 0.25 Tier II
5 NC_000008.11 8 19012584 - PSD3 NNNACCATGGAGGATCTCAG NGG 2 0.2143 Tier II
6 NC_000017.11 17 50119411 + SAMD14 NNNACCCAGCATGATCTCAG NGG 2 0.175 Tier II
7 NC_000009.12 9 33792564 + PRSS3 NNNACCAAGGATCAGCTCAG NGG 2 0.0211 Tier II
8 NC_000001.11 1 7861173 + UTS2 NNNACCAAGGATGATCCCAG NGT 2 0.0114 Tier II
9 NC_000003.12 3 29719718 - RBMS3 NNNTCCAAGGATGATCTCAG NGT 2 0.0103 Tier II
10 NC_000001.11 1 234852278 - LOC107985365 NNNGCCAGGGATGATCTCAG NGG 2 0.4583 Tier III
11 NC_000006.12 6 101227430 + LOC107984041 NNNTCCAAGGATGGTCTCAG NGG 2 0.297 Tier III
12 NC_000003.12 3 161290471 + LOC112268450 NNNACCACAGATGATCTCAG NGG 2 0.2755 Tier III
13 NC_000015.10 15 25464967 - LINC02250 NNNACCAAGGAGGATCTCTG NGG 2 0.2692 Tier III
14 NC_000018.10 18 65140528 - LOC105372168 NNNACCAAGGATGATCTAAG NAG 2 0.1396 Tier III
15 NC_000009.12 9 33792564 + UBE2R2-AS1 NNNACCAAGGATCAGCTCAG NGG 2 0.0211 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 38747990 + Hipk2 NNNACCAAGGATGATCTCAG NGG 0 1.0 Tier I
2 NC_000073.6 7 49453048 + Nav2 NNNACCAAGGCAGATCTCAG NGG 2 0.32 Tier I
3 NC_000085.6 19 4290454 + Grk2 NNNGCCCAGGATGATCTCAG NGG 2 0.2734 Tier I
4 NC_000081.6 15 98841856 + Kmt2d NNNCCCAAGGATGCTCTCAG NGG 2 0.0756 Tier I
5 NC_000068.7 2 11279480 - Prkcq NNNACCAAGGATGACCTCAG NAG 2 0.0707 Tier I
6 NC_000067.6 1 171234239 - Fcer1g NNNACCCAGGATGATCTCAG NCG 2 0.0469 Tier I
7 NC_000070.6 4 44351412 - Melk NNNACCAAGGACGATCTCAA NGG 2 0.8882 Tier II
8 NC_000069.6 3 75937449 - Golim4 NNNACAAAGAATGATCTCAG NGG 2 0.8667 Tier II
9 NC_000074.6 8 12625389 + Tubgcp3 NNNGCCAAGGAAGATCTCAG NGG 2 0.5 Tier II
10 NC_000071.6 5 20822004 + Phtf2 NNNACCAAGTATGTTCTCAG NGG 2 0.4333 Tier II
11 NC_000078.6 12 118881334 - Abcb5 NNNACCAAGTAGGATCTCAG NGG 2 0.4063 Tier II
12 NC_000082.6 16 21221201 + Ephb3 NNNACAAAGCATGATCTCAG NGG 2 0.3714 Tier II
13 NC_000081.6 15 78572564 + Rac2 NNNACCAGGGAGGATCTCAG NGG 2 0.3667 Tier II
14 NC_000080.6 14 44997062 + Ptger2 NNNACCAAGGGTGATCTCTG NGG 2 0.35 Tier II
15 NC_000080.6 14 29618878 - Cacna2d3 NNNACCAAGGCAGATCTCAG NGG 2 0.32 Tier II
16 NC_000074.6 8 71663662 - Unc13a NNNACCCAGGATGTTCTCAG NGG 2 0.2333 Tier II
17 NC_000068.7 2 4927807 + Phyh NNNACCAAGGATGCTATCAG NGG 2 0.2143 Tier II
18 NC_000075.6 9 102138196 - Ephb1 NNNACCTAGGATGGTCTCAG NGG 2 0.2042 Tier II
19 NC_000076.6 10 41579604 + Ccdc162 NNNACCAAGGATGATCTCAT NAG 2 0.1815 Tier II
20 NC_000076.6 10 19745799 + Il20ra NNNTCCAAGGATGATCTCAG NAG 2 0.165 Tier II
21 NC_000084.6 18 37904177 - Diaph1 NNNACCAAGGATGATGTCTG NGG 2 0.0828 Tier II
22 NC_000076.6 10 67211605 - Jmjd1c NNNACCAAGGATAATCTCAG NGT 2 0.0149 Tier II
23 NC_000079.6 13 46295268 + Stmnd1 NNNGCCAAGGATGATCTCAG NGC 2 0.0139 Tier II
24 NC_000071.6 5 27127931 + Dpp6 NNNACCAAGGAAGATCTCAG NGT 2 0.0129 Tier II
25 NC_000067.6 1 59594891 + Gm973 NNNACCCAGGATGATCTCAG NGC 2 0.0097 Tier II
26 NC_000072.6 6 136167897 + Grin2b NNNACCAAGGATGAGCTGAG NGG 2 0.0067 Tier II
27 NC_000072.6 6 38747990 + 4930502C15Rik NNNACCAAGGATGATCTCAG NGG 0 1.0 Tier III
28 NC_000072.6 6 69643819 - Igk NNNATCAAGGAAGATCTCAG NGG 2 0.5091 Tier III
29 NC_000084.6 18 16302412 + Gm30765 NNNATCAGGGATGATCTCAG NGG 2 0.4667 Tier III
30 NC_000074.6 8 68996401 + Gm33052 NNNACCAAGGGTGATCTCAT NGG 2 0.455 Tier III
31 NC_000074.6 8 68996401 + Gm33103 NNNACCAAGGGTGATCTCAT NGG 2 0.455 Tier III
32 NC_000080.6 14 25700821 - Ppifos NNNAGCAAGGATGTTCTCAG NGG 2 0.32 Tier III
33 NC_000067.6 1 59594891 + Gm39648 NNNACCCAGGATGATCTCAG NGC 2 0.0097 Tier III
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Other clones with same target sequence:

(none)