Construct: sgRNA BRDN0001148697
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAATGATACTGGATAGTCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAGI3 (260425)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76551
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
113642372 |
+ |
MAGI3 |
NNNTGATACTGGATAGTCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
56670780 |
- |
DST |
NNNTGTTACTGGAGAGTCAG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000004.12 |
4 |
92882487 |
+ |
GRID2 |
NNNCAATACTGGATAGTCAG |
NGG |
2 |
0.5608 |
Tier II |
| 4 |
NC_000015.10 |
15 |
101206975 |
- |
CHSY1 |
NNNTGGTACTGGATAGCCAG |
NGG |
2 |
0.5042 |
Tier II |
| 5 |
NC_000003.12 |
3 |
51664760 |
+ |
RAD54L2 |
NNNTGAGACTGGATAGTTAG |
NGG |
2 |
0.3782 |
Tier II |
| 6 |
NC_000009.12 |
9 |
77813735 |
- |
GNAQ |
NNNTGCTACTGGGTAGTCAG |
NGG |
2 |
0.2964 |
Tier II |
| 7 |
NC_000015.10 |
15 |
93057139 |
- |
RGMA |
NNNTGATCCTGGACAGTCAG |
NGG |
2 |
0.1224 |
Tier II |
| 8 |
NC_000016.10 |
16 |
10077536 |
- |
GRIN2A |
NNNTGATACTGGATTGTAAG |
NGG |
2 |
0.1077 |
Tier II |
| 9 |
NC_000008.11 |
8 |
78775834 |
+ |
IL7 |
NNNTGATACAGGATAGTCAG |
NGC |
2 |
0.019 |
Tier II |
| 10 |
NC_000004.12 |
4 |
147827343 |
+ |
ARHGAP10 |
NNNTGATACTGGAGAGTAAG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000017.11 |
17 |
33634491 |
+ |
ASIC2 |
NNNTGATACTGGATATTCAG |
NGA |
2 |
0.0 |
Tier II |
| 12 |
NC_000018.10 |
18 |
13394753 |
- |
LDLRAD4 |
NNNTGATCCTGGATACTCAG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000015.10 |
15 |
65979571 |
+ |
MEGF11 |
NNNTGTTACTGGAGAGTCAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000007.14 |
7 |
10144333 |
+ |
LOC105375149 |
NNNTGATAATGGTTAGTCAG |
NGG |
2 |
0.2571 |
Tier III |
| 15 |
NC_000003.12 |
3 |
66892854 |
+ |
LOC105377144 |
NNNTGGTACTGGTTAGTCAG |
NGG |
2 |
0.2143 |
Tier III |
| 16 |
NC_000019.10 |
19 |
56758294 |
- |
LOC105372472 |
NNNTGATACTGGATTGGCAG |
NGG |
2 |
0.0235 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
104050938 |
- |
Magi3 |
NNNTGATATTGGATAGCCAG |
NGG |
2 |
0.6176 |
Tier I |
| 2 |
NC_000068.7 |
2 |
144486053 |
+ |
Dzank1 |
NNNTGAAACTGAATAGTCAG |
NGG |
2 |
0.8167 |
Tier II |
| 3 |
NC_000069.6 |
3 |
136850867 |
+ |
Ppp3ca |
NNNTGATACTAGAAAGTCAG |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000072.6 |
6 |
46352086 |
- |
Cntnap2 |
NNNTGATAATGGATAGTCTG |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000070.6 |
4 |
141101912 |
- |
Spata21 |
NNNTGAGACTGGATGGTCAG |
NGG |
2 |
0.3824 |
Tier II |
| 6 |
NC_000069.6 |
3 |
108348963 |
+ |
Sort1 |
NNNTCATACTGGATAGTCAC |
NGG |
2 |
0.3367 |
Tier II |
| 7 |
NC_000077.6 |
11 |
95903876 |
- |
B4galnt2 |
NNNTGTTTCTGGATAGTCAG |
NGG |
2 |
0.3061 |
Tier II |
| 8 |
NC_000081.6 |
15 |
6639789 |
+ |
Fyb |
NNNTGCTACTGGATAGACAG |
NGG |
2 |
0.2424 |
Tier II |
| 9 |
NC_000068.7 |
2 |
49502784 |
+ |
Epc2 |
NNNTGATACTGGATAGCCAG |
NTG |
2 |
0.0275 |
Tier II |
| 10 |
NC_000081.6 |
15 |
87636979 |
+ |
Tafa5 |
NNNTGATCCTGGATAGTCAG |
NTG |
2 |
0.0167 |
Tier II |
| 11 |
NC_000071.6 |
5 |
5681205 |
+ |
Steap2 |
NNNTGATAGTGGATAGTCAG |
NGT |
2 |
0.01 |
Tier II |
| 12 |
NC_000077.6 |
11 |
93433029 |
- |
Car10 |
NNNTGATACTGGAGATTCAG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000069.6 |
3 |
26079560 |
- |
Nlgn1 |
NNNTGATACTGTATATTCAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000080.6 |
14 |
62363117 |
- |
Rnaseh2b |
NNNTGATTCTGGATATTCAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000081.6 |
15 |
6639789 |
+ |
Gm2245 |
NNNTGCTACTGGATAGACAG |
NGG |
2 |
0.2424 |
Tier III |
| 16 |
NC_000080.6 |
14 |
104659796 |
+ |
D130009I18Rik |
NNNTGGTACTGGATAGTCAG |
NTG |
2 |
0.0278 |
Tier III |
| 17 |
NC_000071.6 |
5 |
5681205 |
+ |
Gm40262 |
NNNTGATAGTGGATAGTCAG |
NGT |
2 |
0.01 |
Tier III |
Other clones with same target sequence:
(none)
