Construct: sgRNA BRDN0001148699
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTATGAGAAGCTCATAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GCK (2645)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77582
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
44146604 |
- |
GCK |
NNNTATGAGAAGCTCATAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
98679554 |
- |
SNX7 |
NNNTATTAGAAGCTCATAGG |
NGG |
1 |
0.5714 |
Tier II |
| 3 |
NC_000011.10 |
11 |
4973918 |
+ |
MMP26 |
NNNGATGAGAAGCTCATAAG |
NGG |
2 |
0.4464 |
Tier II |
| 4 |
NC_000010.11 |
10 |
116857316 |
- |
ENO4 |
NNNTATGAGGAGCTCACAGG |
NGG |
2 |
0.3922 |
Tier II |
| 5 |
NC_000011.10 |
11 |
28123134 |
+ |
METTL15 |
NNNTATGAGAAGCTCATAGA |
NAG |
2 |
0.2431 |
Tier II |
| 6 |
NC_000017.11 |
17 |
11742681 |
- |
DNAH9 |
NNNTATGAGAAGCTCAAAGC |
NGG |
2 |
0.2286 |
Tier II |
| 7 |
NC_000013.11 |
13 |
66606401 |
+ |
PCDH9 |
NNNTAGGAGAAGCTCATAGG |
NAG |
2 |
0.1481 |
Tier II |
| 8 |
NC_000006.12 |
6 |
124550328 |
- |
NKAIN2 |
NNNTATGAAAAGGTCATAGG |
NGG |
2 |
0.0877 |
Tier II |
| 9 |
NC_000003.12 |
3 |
37154701 |
- |
LRRFIP2 |
NNNTATGAGAAGCTCATTGG |
NGA |
2 |
0.0347 |
Tier II |
| 10 |
NC_000001.11 |
1 |
54721707 |
+ |
TTC4 |
NNNTTTGAGAAGCTCATAGG |
NGC |
2 |
0.0081 |
Tier II |
| 11 |
NC_000003.12 |
3 |
35248791 |
+ |
LOC101928135 |
NNNTATGAAATGCTCATAGG |
NGG |
2 |
0.1978 |
Tier III |
| 12 |
NC_000013.11 |
13 |
66606401 |
+ |
LOC105370247 |
NNNTAGGAGAAGCTCATAGG |
NAG |
2 |
0.1481 |
Tier III |
| 13 |
NC_000001.11 |
1 |
206895351 |
+ |
LOC105372879 |
NNNTATGATAAGCTCATCGG |
NGG |
2 |
0.1016 |
Tier III |
| 14 |
NC_000013.11 |
13 |
73654646 |
- |
LINC00393 |
NNNTATGAGAAGCTCATATG |
NGA |
2 |
0.0463 |
Tier III |
| 15 |
NC_000013.11 |
13 |
30903480 |
+ |
TEX26-AS1 |
NNNTATGTGAAGCTCATAGG |
NTG |
2 |
0.0167 |
Tier III |
| 16 |
NC_000001.11 |
1 |
54721707 |
+ |
MROH7-TTC4 |
NNNTTTGAGAAGCTCATAGG |
NGC |
2 |
0.0081 |
Tier III |
| 17 |
NC_000012.12 |
12 |
52720668 |
+ |
KRT126P |
NNNTATGAGAAGCACCTAGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
16034070 |
- |
Bicra |
NNNGATGAGTAGCTCATAGG |
NGG |
2 |
0.5515 |
Tier II |
| 2 |
NC_000083.6 |
17 |
86220960 |
+ |
Prkce |
NNNTGTGAGAATCTCATAGG |
NGG |
2 |
0.2769 |
Tier II |
| 3 |
NC_000067.6 |
1 |
181657083 |
+ |
Dnah14 |
NNNTATGAGAGTCTCATAGG |
NGG |
2 |
0.25 |
Tier II |
| 4 |
NC_000078.6 |
12 |
82908184 |
+ |
Rgs6 |
NNNTTTGATAAGCTCATAGG |
NGG |
2 |
0.1939 |
Tier II |
| 5 |
NC_000086.7 |
X |
87655188 |
+ |
Il1rapl1 |
NNNTATGTGAAGGTCATAGG |
NGG |
2 |
0.0584 |
Tier II |
| 6 |
NC_000079.6 |
13 |
75131473 |
- |
Pcsk1 |
NNNTCTGAGAAGCTCATAGG |
NGA |
2 |
0.0347 |
Tier II |
| 7 |
NC_000067.6 |
1 |
70132511 |
- |
Spag16 |
NNNTATCAGAAGCTCATAGG |
NTG |
2 |
0.0268 |
Tier II |
| 8 |
NC_000071.6 |
5 |
127299802 |
+ |
Tmem132c |
NNNTCTGAGAAGCTCATAGG |
NTG |
2 |
0.0195 |
Tier II |
| 9 |
NC_000074.6 |
8 |
119955989 |
+ |
Usp10 |
NNNTAAGAGAAGCTCCTAGG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000071.6 |
5 |
100214145 |
+ |
2310034O05Rik |
NNNTATCAGAAGCTCATAGG |
NAG |
2 |
0.1782 |
Tier III |
| 11 |
NC_000071.6 |
5 |
100214145 |
+ |
Gm36304 |
NNNTATCAGAAGCTCATAGG |
NAG |
2 |
0.1782 |
Tier III |
| 12 |
NC_000084.6 |
18 |
59839310 |
- |
Gm35234 |
NNNAATGAGAAGCTCAGAGG |
NGG |
2 |
0.056 |
Tier III |
Other clones with same target sequence:
(none)
