Construct: sgRNA BRDN0001148706

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGGTCATCAAAGACTACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K19 (80122)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76695

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 134987536 + MAP3K19 NNNGTCATCAAAGACTACAC NGG 0 1.0 Tier I
2 NC_000019.10 19 48039306 + CABP5 NNNGTCATCAAAGTCTACAC NGC 2 0.0119 Tier I
3 NC_000012.12 12 32470803 - FGD4 NNNGTCAGCAAGGACTACAC NGG 2 0.5296 Tier II
4 NC_000006.12 6 130202894 - SAMD3 NNNGGCATCAAAGACCACAC NGG 2 0.4267 Tier II
5 NC_000005.10 5 39301832 - C9 NNNTTCATAAAAGACTACAC NGG 2 0.3117 Tier II
6 NC_000002.12 2 110813293 + ACOXL NNNGTCATCAGAGGCTACAC NGG 2 0.3033 Tier II
7 NC_000003.12 3 114390839 + ZBTB20 NNNGCCATCAAATACTACAC NGG 2 0.3 Tier II
8 NC_000005.10 5 7882256 - MTRR NNNCTCATCAAAGACTACAA NGG 2 0.2647 Tier II
9 NC_000008.11 8 55434864 + XKR4 NNNGTCTTCAAAGGCTACAC NGG 2 0.2042 Tier II
10 NC_000004.12 4 86315066 - MAPK10 NNNGTCATCAAAGACTATAT NGG 2 0.1929 Tier II
11 NC_000010.11 10 17654090 - STAM NNNGTCAGCAAAGACTTCAC NGG 2 0.0978 Tier II
12 NC_000001.11 1 220000012 - EPRS1 NNNGTCATCAAAGACTCAAC NGG 2 0.095 Tier II
13 NC_000009.12 9 83382817 + FRMD3 NNNGTCATGAAAGACTTCAC NGG 2 0.0825 Tier II
14 NC_000003.12 3 55782235 + ERC2 NNNGTCATCAAAGACCACAG NGG 2 0.0392 Tier II
15 NC_000001.11 1 240920101 - RGS7 NNNGTCATCAAAGATTACAA NGG 2 0.0333 Tier II
16 NC_000005.10 5 154251674 - GALNT10 NNNGTCATCAAAGACTACTG NGG 2 0.0317 Tier II
17 NC_000003.12 3 188676848 - LPP NNNGTCATCAAAGACTACAT NTG 2 0.0117 Tier II
18 NC_000011.10 11 16871287 + PLEKHA7 NNNGTCATCAAAGATTTCAC NGG 2 0.0089 Tier II
19 NC_000006.12 6 136050852 - PDE7B NNNGTCATCAAAGATTACAC NGT 2 0.0011 Tier II
20 NC_000014.9 14 90508174 + LOC124903359 NNNGTCATGAAAGACAACAC NGG 2 0.5628 Tier III
21 NC_000001.11 1 240920101 - HNRNPA1P42 NNNGTCATCAAAGATTACAA NGG 2 0.0333 Tier III
22 NC_000017.11 17 44920389 - LOC105371793 NNNGTCATCAAAGACTGCAC NGC 2 0.0039 Tier III
23 NC_000006.12 6 136050852 - PDE7B-AS1 NNNGTCATCAAAGATTACAC NGT 2 0.0011 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 57529514 - Mfap3 NNNGTCATCAAAGGCTACGC NGG 2 0.175 Tier I
2 NC_000078.6 12 82817957 + Rgs6 NNNATCATCAAAGACAACAC NGG 2 0.8182 Tier II
3 NC_000086.7 X 73879557 + L1cam NNNGTCAGCAAAGACAACAC NGG 2 0.6667 Tier II
4 NC_000080.6 14 7939961 - Flnb NNNGTCATCAAAGACCACAC NGG 1 0.6667 Tier II
5 NC_000074.6 8 17115024 + Csmd1 NNNGTCATCAAGGACAACAC NGG 2 0.6566 Tier II
6 NC_000071.6 5 51480122 + Ppargc1a NNNGTCACCAAACACTACAC NGG 2 0.4211 Tier II
7 NC_000080.6 14 39059927 + Nrg3 NNNGTGAACAAAGACTACAC NGG 2 0.4 Tier II
8 NC_000072.6 6 65856928 - Prdm5 NNNGTAATCAAACACTACAC NGG 2 0.391 Tier II
9 NC_000077.6 11 113269979 - Slc39a11 NNNGTCGTCAAAGACTAAAC NGG 2 0.3801 Tier II
10 NC_000082.6 16 50323220 + Bbx NNNGTCATCCAGGACTACAC NGG 2 0.2407 Tier II
11 NC_000086.7 X 161320484 - Gm15262 NNNGTTATCAAAGACTACAC NAG 2 0.2407 Tier II
12 NC_000071.6 5 142138186 - Sdk1 NNNGTCATCACACACTACAC NGG 2 0.1684 Tier II
13 NC_000074.6 8 86850183 + N4bp1 NNNGACATCCAAGACTACAC NGG 2 0.1667 Tier II
14 NC_000080.6 14 29676760 + Cacna2d3 NNNGTCATCAAAGACGACAC NGA 2 0.024 Tier II
15 NC_000081.6 15 53318086 + Ext1 NNNGTCATAAAAGACTACAC NGT 2 0.0138 Tier II
16 NC_000074.6 8 99598421 - A330008L17Rik NNNGCCATCATAGACTACAC NGG 2 0.3077 Tier III
17 NC_000074.6 8 86850183 + Gm33877 NNNGACATCCAAGACTACAC NGG 2 0.1667 Tier III
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Other clones with same target sequence:

(none)