Construct: sgRNA BRDN0001148706
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGTCATCAAAGACTACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K19 (80122)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76695
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000002.12 |
2 |
134987536 |
+ |
MAP3K19 |
NNNGTCATCAAAGACTACAC |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000019.10 |
19 |
48039306 |
+ |
CABP5 |
NNNGTCATCAAAGTCTACAC |
NGC |
2 |
0.0119 |
Tier I |
3 |
NC_000012.12 |
12 |
32470803 |
- |
FGD4 |
NNNGTCAGCAAGGACTACAC |
NGG |
2 |
0.5296 |
Tier II |
4 |
NC_000006.12 |
6 |
130202894 |
- |
SAMD3 |
NNNGGCATCAAAGACCACAC |
NGG |
2 |
0.4267 |
Tier II |
5 |
NC_000005.10 |
5 |
39301832 |
- |
C9 |
NNNTTCATAAAAGACTACAC |
NGG |
2 |
0.3117 |
Tier II |
6 |
NC_000002.12 |
2 |
110813293 |
+ |
ACOXL |
NNNGTCATCAGAGGCTACAC |
NGG |
2 |
0.3033 |
Tier II |
7 |
NC_000003.12 |
3 |
114390839 |
+ |
ZBTB20 |
NNNGCCATCAAATACTACAC |
NGG |
2 |
0.3 |
Tier II |
8 |
NC_000005.10 |
5 |
7882256 |
- |
MTRR |
NNNCTCATCAAAGACTACAA |
NGG |
2 |
0.2647 |
Tier II |
9 |
NC_000008.11 |
8 |
55434864 |
+ |
XKR4 |
NNNGTCTTCAAAGGCTACAC |
NGG |
2 |
0.2042 |
Tier II |
10 |
NC_000004.12 |
4 |
86315066 |
- |
MAPK10 |
NNNGTCATCAAAGACTATAT |
NGG |
2 |
0.1929 |
Tier II |
11 |
NC_000010.11 |
10 |
17654090 |
- |
STAM |
NNNGTCAGCAAAGACTTCAC |
NGG |
2 |
0.0978 |
Tier II |
12 |
NC_000001.11 |
1 |
220000012 |
- |
EPRS1 |
NNNGTCATCAAAGACTCAAC |
NGG |
2 |
0.095 |
Tier II |
13 |
NC_000009.12 |
9 |
83382817 |
+ |
FRMD3 |
NNNGTCATGAAAGACTTCAC |
NGG |
2 |
0.0825 |
Tier II |
14 |
NC_000003.12 |
3 |
55782235 |
+ |
ERC2 |
NNNGTCATCAAAGACCACAG |
NGG |
2 |
0.0392 |
Tier II |
15 |
NC_000001.11 |
1 |
240920101 |
- |
RGS7 |
NNNGTCATCAAAGATTACAA |
NGG |
2 |
0.0333 |
Tier II |
16 |
NC_000005.10 |
5 |
154251674 |
- |
GALNT10 |
NNNGTCATCAAAGACTACTG |
NGG |
2 |
0.0317 |
Tier II |
17 |
NC_000003.12 |
3 |
188676848 |
- |
LPP |
NNNGTCATCAAAGACTACAT |
NTG |
2 |
0.0117 |
Tier II |
18 |
NC_000011.10 |
11 |
16871287 |
+ |
PLEKHA7 |
NNNGTCATCAAAGATTTCAC |
NGG |
2 |
0.0089 |
Tier II |
19 |
NC_000006.12 |
6 |
136050852 |
- |
PDE7B |
NNNGTCATCAAAGATTACAC |
NGT |
2 |
0.0011 |
Tier II |
20 |
NC_000014.9 |
14 |
90508174 |
+ |
LOC124903359 |
NNNGTCATGAAAGACAACAC |
NGG |
2 |
0.5628 |
Tier III |
21 |
NC_000001.11 |
1 |
240920101 |
- |
HNRNPA1P42 |
NNNGTCATCAAAGATTACAA |
NGG |
2 |
0.0333 |
Tier III |
22 |
NC_000017.11 |
17 |
44920389 |
- |
LOC105371793 |
NNNGTCATCAAAGACTGCAC |
NGC |
2 |
0.0039 |
Tier III |
23 |
NC_000006.12 |
6 |
136050852 |
- |
PDE7B-AS1 |
NNNGTCATCAAAGATTACAC |
NGT |
2 |
0.0011 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000077.6 |
11 |
57529514 |
- |
Mfap3 |
NNNGTCATCAAAGGCTACGC |
NGG |
2 |
0.175 |
Tier I |
2 |
NC_000078.6 |
12 |
82817957 |
+ |
Rgs6 |
NNNATCATCAAAGACAACAC |
NGG |
2 |
0.8182 |
Tier II |
3 |
NC_000086.7 |
X |
73879557 |
+ |
L1cam |
NNNGTCAGCAAAGACAACAC |
NGG |
2 |
0.6667 |
Tier II |
4 |
NC_000080.6 |
14 |
7939961 |
- |
Flnb |
NNNGTCATCAAAGACCACAC |
NGG |
1 |
0.6667 |
Tier II |
5 |
NC_000074.6 |
8 |
17115024 |
+ |
Csmd1 |
NNNGTCATCAAGGACAACAC |
NGG |
2 |
0.6566 |
Tier II |
6 |
NC_000071.6 |
5 |
51480122 |
+ |
Ppargc1a |
NNNGTCACCAAACACTACAC |
NGG |
2 |
0.4211 |
Tier II |
7 |
NC_000080.6 |
14 |
39059927 |
+ |
Nrg3 |
NNNGTGAACAAAGACTACAC |
NGG |
2 |
0.4 |
Tier II |
8 |
NC_000072.6 |
6 |
65856928 |
- |
Prdm5 |
NNNGTAATCAAACACTACAC |
NGG |
2 |
0.391 |
Tier II |
9 |
NC_000077.6 |
11 |
113269979 |
- |
Slc39a11 |
NNNGTCGTCAAAGACTAAAC |
NGG |
2 |
0.3801 |
Tier II |
10 |
NC_000082.6 |
16 |
50323220 |
+ |
Bbx |
NNNGTCATCCAGGACTACAC |
NGG |
2 |
0.2407 |
Tier II |
11 |
NC_000086.7 |
X |
161320484 |
- |
Gm15262 |
NNNGTTATCAAAGACTACAC |
NAG |
2 |
0.2407 |
Tier II |
12 |
NC_000071.6 |
5 |
142138186 |
- |
Sdk1 |
NNNGTCATCACACACTACAC |
NGG |
2 |
0.1684 |
Tier II |
13 |
NC_000074.6 |
8 |
86850183 |
+ |
N4bp1 |
NNNGACATCCAAGACTACAC |
NGG |
2 |
0.1667 |
Tier II |
14 |
NC_000080.6 |
14 |
29676760 |
+ |
Cacna2d3 |
NNNGTCATCAAAGACGACAC |
NGA |
2 |
0.024 |
Tier II |
15 |
NC_000081.6 |
15 |
53318086 |
+ |
Ext1 |
NNNGTCATAAAAGACTACAC |
NGT |
2 |
0.0138 |
Tier II |
16 |
NC_000074.6 |
8 |
99598421 |
- |
A330008L17Rik |
NNNGCCATCATAGACTACAC |
NGG |
2 |
0.3077 |
Tier III |
17 |
NC_000074.6 |
8 |
86850183 |
+ |
Gm33877 |
NNNGACATCCAAGACTACAC |
NGG |
2 |
0.1667 |
Tier III |
Other clones with same target sequence:
(none)