Construct: sgRNA BRDN0001148832
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCCTAACTTTCGGATCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRPK1 (6732)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77495
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
35888872 |
+ |
SRPK1 |
NNNCTAACTTTCGGATCACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
10247109 |
- |
SNAP25 |
NNNCTAACTTTTGGGTCACA |
NGG |
2 |
0.35 |
Tier II |
| 3 |
NC_000005.10 |
5 |
103187754 |
+ |
PPIP5K2 |
NNNCTAATTTTCTGATCACA |
NGG |
2 |
0.2625 |
Tier II |
| 4 |
NC_000008.11 |
8 |
142664135 |
+ |
JRK |
NNNCTCACTTTCGGATGACA |
NGG |
2 |
0.0267 |
Tier II |
| 5 |
NC_000003.12 |
3 |
65753917 |
+ |
MAGI1 |
NNNCTAACTTTCGGATCATA |
NGC |
2 |
0.0103 |
Tier II |
| 6 |
NC_000009.12 |
9 |
98870747 |
+ |
LOC102724684 |
NNNCTAACTTCAGGATCACA |
NGG |
2 |
0.4762 |
Tier III |
| 7 |
NC_000003.12 |
3 |
65753917 |
+ |
LOC107986018 |
NNNCTAACTTTCGGATCATA |
NGC |
2 |
0.0103 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
96380644 |
- |
Tenm4 |
NNNCAAACTTTCAGATCACA |
NGG |
2 |
0.4615 |
Tier II |
| 2 |
NC_000073.6 |
7 |
78306004 |
+ |
Ntrk3 |
NNNCTAACTTTGGAATCACA |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000072.6 |
6 |
141264663 |
+ |
Pde3a |
NNNCTAACTTTTGGCTCACA |
NGG |
2 |
0.1469 |
Tier II |
| 4 |
NC_000078.6 |
12 |
70201488 |
+ |
Pygl |
NNNCTAACTTTCTGAGCACA |
NGG |
2 |
0.1038 |
Tier II |
| 5 |
NC_000068.7 |
2 |
92819590 |
- |
Gm13791 |
NNNATAACTCTCGGATCACA |
NGG |
2 |
0.4491 |
Tier III |
| 6 |
NC_000068.7 |
2 |
92819590 |
- |
Gm39880 |
NNNATAACTCTCGGATCACA |
NGG |
2 |
0.4491 |
Tier III |
| 7 |
NC_000082.6 |
16 |
92156242 |
+ |
Gm41495 |
NNNCTAACTTTCTCATCACA |
NGG |
2 |
0.1286 |
Tier III |
| 8 |
NC_000082.6 |
16 |
92156242 |
+ |
Gm41496 |
NNNCTAACTTTCTCATCACA |
NGG |
2 |
0.1286 |
Tier III |
Other clones with same target sequence:
(none)
