Construct: sgRNA BRDN0001148834

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGACGAGGATGACACGACGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAST1 (22983)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76123

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 12866704 + MAST1 NNNCGAGGATGACACGACGG NGG 0 1.0 Tier I
2 NC_000023.11 X 47224731 - CDK16 NNNCTAGGCTGACACGACGG NGG 2 0.1714 Tier I
3 NC_000006.12 6 8784229 + LOC100506207 NNNTGAGGATGACACGATGG NGG 2 0.5143 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 31205060 - Ppm1g NNNCGAGGATGACACGGAGG NGG 2 0.095 Tier I
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Other clones with same target sequence:

(none)