Construct: sgRNA BRDN0001148836
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGCTTCCATCAAATGGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SIK3 (23387)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75756
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
116896334 |
+ |
SIK3 |
NNNGCTTCCATCAAATGGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
3825000 |
+ |
GLIS3 |
NNNGCTTCCATAAAATGACA |
NGG |
2 |
0.4945 |
Tier II |
| 3 |
NC_000002.12 |
2 |
165382963 |
+ |
SCN2A |
NNNGTTTCCATCAAATGACA |
NGG |
2 |
0.4406 |
Tier II |
| 4 |
NC_000016.10 |
16 |
24147362 |
- |
PRKCB |
NNNGCTTCCATCAAGTGGCT |
NGG |
2 |
0.39 |
Tier II |
| 5 |
NC_000011.10 |
11 |
17516078 |
- |
USH1C |
NNNGCTGACATCAAATGGCA |
NGG |
2 |
0.3824 |
Tier II |
| 6 |
NC_000016.10 |
16 |
8905759 |
- |
USP7 |
NNNGCTTCCATAAAATGCCA |
NGG |
2 |
0.3401 |
Tier II |
| 7 |
NC_000008.11 |
8 |
105780227 |
- |
ZFPM2 |
NNNGCTTCCACCAAATGGAA |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000007.14 |
7 |
104848249 |
- |
LHFPL3 |
NNNGCTTCCCTCAAATGACA |
NGG |
2 |
0.2308 |
Tier II |
| 9 |
NC_000008.11 |
8 |
13307306 |
+ |
DLC1 |
NNNGCTTCCAACAAATGGCA |
NAG |
2 |
0.1944 |
Tier II |
| 10 |
NC_000007.14 |
7 |
101989615 |
- |
CUX1 |
NNNGCTTCCATCCAATGACA |
NGG |
2 |
0.1457 |
Tier II |
| 11 |
NC_000022.11 |
22 |
28939713 |
- |
ZNRF3 |
NNNGCTTCCATTAAATTGCA |
NGG |
2 |
0.1346 |
Tier II |
| 12 |
NC_000003.12 |
3 |
58743262 |
+ |
CFAP20DC |
NNNGCTTCCAACAAATGGCA |
NGA |
2 |
0.0521 |
Tier II |
| 13 |
NC_000002.12 |
2 |
137518050 |
+ |
THSD7B |
NNNGCTTCCATCAAATGGAA |
NTG |
2 |
0.0167 |
Tier II |
| 14 |
NC_000005.10 |
5 |
88730182 |
- |
MEF2C |
NNNGCTTCCCTCAAATGGCA |
NGT |
2 |
0.0054 |
Tier II |
| 15 |
NC_000001.11 |
1 |
227241659 |
- |
CDC42BPA |
NNNGCTTCCATCAAATGGCA |
NCC |
2 |
0.0 |
Tier II |
| 16 |
NC_000006.12 |
6 |
135715703 |
- |
AHI1-DT |
NNNGCTTCAATCTAATGGCA |
NGG |
2 |
0.2571 |
Tier III |
| 17 |
NC_000010.11 |
10 |
64045570 |
- |
LOC124902439 |
NNNGCTTCCATAAAATGGGA |
NGG |
2 |
0.0893 |
Tier III |
| 18 |
NC_000012.12 |
12 |
83891406 |
- |
LOC107984536 |
NNNGCTTCCAGCAAATGGCA |
NTG |
2 |
0.0156 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
7014823 |
+ |
Fermt3 |
NNNGCTCCCACCAAATGGCA |
NGG |
2 |
0.4583 |
Tier I |
| 2 |
NC_000075.6 |
9 |
46178475 |
- |
Sik3 |
NNNGCTCCCATCAAACGGCA |
NGG |
2 |
0.4583 |
Tier I |
| 3 |
NC_000078.6 |
12 |
90257220 |
- |
Nrxn3 |
NNNGCTACCATCAAATAGCA |
NGG |
2 |
0.8167 |
Tier II |
| 4 |
NC_000080.6 |
14 |
10200375 |
- |
Fhit |
NNNACTTCCATCAAATGGCT |
NGG |
2 |
0.54 |
Tier II |
| 5 |
NC_000079.6 |
13 |
11623857 |
+ |
Ryr2 |
NNNGCGTCCATCAAATGGCG |
NGG |
2 |
0.437 |
Tier II |
| 6 |
NC_000074.6 |
8 |
22763691 |
+ |
Plat |
NNNGCTTCCATCAAGTGCCA |
NGG |
2 |
0.3095 |
Tier II |
| 7 |
NC_000075.6 |
9 |
21201478 |
+ |
Pde4a |
NNNGCATCCATCAAAGGGCA |
NGG |
2 |
0.3 |
Tier II |
| 8 |
NC_000082.6 |
16 |
60404608 |
- |
Epha6 |
NNNGCTTCCATCAGATGGCT |
NGG |
2 |
0.28 |
Tier II |
| 9 |
NC_000078.6 |
12 |
117203447 |
+ |
Ptprn2 |
NNNGTTTCCCTCAAATGGCA |
NGG |
2 |
0.2121 |
Tier II |
| 10 |
NC_000073.6 |
7 |
116152725 |
- |
Plekha7 |
NNNGCTTCCATCAGAGGGCA |
NGG |
2 |
0.1615 |
Tier II |
| 11 |
NC_000070.6 |
4 |
133608606 |
+ |
Zdhhc18 |
NNNGCTTCCCTCAGATGGCA |
NGG |
2 |
0.1556 |
Tier II |
| 12 |
NC_000083.6 |
17 |
45587397 |
+ |
Slc29a1 |
NNNGTTTCCATCCAATGGCA |
NGG |
2 |
0.134 |
Tier II |
| 13 |
NC_000075.6 |
9 |
95057485 |
+ |
Slc9a9 |
NNNGCTTCCATCACATGGTA |
NGG |
2 |
0.0989 |
Tier II |
| 14 |
NC_000084.6 |
18 |
12517596 |
- |
Lama3 |
NNNGCTGCCATCAAATGGGA |
NGG |
2 |
0.0735 |
Tier II |
| 15 |
NC_000079.6 |
13 |
71769713 |
+ |
Gm40999 |
NNNGCTTCCATCAAATGGGA |
NGA |
2 |
0.0087 |
Tier III |
Other clones with same target sequence:
(none)
