Construct: sgRNA BRDN0001148841

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGCAAGCTATGATGCCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXOSC10 (5394)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77989

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 11079813 + EXOSC10 NNNCAAGCTATGATGCCCTG NGG 0 1.0 Tier I
2 NC_000008.11 8 144113678 + WDR97 NNNCAAGCCAGGATGCCCTG NGG 2 0.3692 Tier I
3 NC_000002.12 2 28399144 - FOSL2 NNNCAAGCCATGAAGCCCTG NGG 2 0.5714 Tier II
4 NC_000015.10 15 76369275 + SCAPER NNNAAATCTATGATGCCCTG NGG 2 0.4812 Tier II
5 NC_000001.11 1 65962053 - PDE4B NNNTAAGCTATGATGCTCTG NGG 2 0.3733 Tier II
6 NC_000010.11 10 98202454 + R3HCC1L NNNCAAGATGTGATGCCCTG NGG 2 0.3611 Tier II
7 NC_000017.11 17 56098953 + ANKFN1 NNNCAAGCTCAGATGCCCTG NGG 2 0.25 Tier II
8 NC_000015.10 15 63234698 + RAB8B NNNCGAGCTCTGATGCCCTG NGG 2 0.24 Tier II
9 NC_000018.10 18 45032690 + SETBP1 NNNCAAGCTATGAGACCCTG NGG 2 0.0 Tier II
10 NC_000013.11 13 76743470 - LOC105370265 NNNCAGGCTATGATGCCCTG NGG 1 0.7143 Tier III
11 NC_000002.12 2 18835393 - LOC105373456 NNNCAAGCTATGATGACCTT NGG 2 0.7 Tier III
12 NC_000006.12 6 113991764 + HDAC2-AS2 NNNCAAGCCATCATGCCCTG NGG 2 0.4887 Tier III
13 NC_000023.11 X 94901321 - LOC107985710 NNNCAACCTATGAAGCCCTG NGG 2 0.4256 Tier III
14 NC_000003.12 3 80677053 + LOC105377177 NNNAAAGCTATGATGCCCTC NGG 2 0.3609 Tier III
15 NC_000015.10 15 78703634 - LOC105370913 NNNCAGGCTATTATGCCCTG NGG 2 0.2747 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 148570397 - Exosc10 NNNCAAGCTATGATGCCTTG NGG 1 0.6429 Tier I
2 NC_000075.6 9 120415577 + Myrip NNNCAAGCTTTGATGCCCTA NGG 2 0.8272 Tier II
3 NC_000077.6 11 95874009 + B4galnt2 NNNCAGACTATGATGCCCTG NGG 2 0.7143 Tier II
4 NC_000079.6 13 104096460 - Nln NNNAGAGCTATGATGCCCTG NGG 2 0.6063 Tier II
5 NC_000071.6 5 92944645 - Shroom3 NNNCAAGCTATTATACCCTG NGG 2 0.362 Tier II
6 NC_000071.6 5 92307177 - Sdad1 NNNCAAGCTTGGATGCCCTG NGG 2 0.3529 Tier II
7 NC_000073.6 7 74281355 - Slco3a1 NNNCAAGCTCTGATACCCTG NGG 2 0.3137 Tier II
8 NC_000070.6 4 132979451 - Fgr NNNCAAGCTCTGATGCCCTA NGG 2 0.3125 Tier II
9 NC_000073.6 7 120711818 + Mosmo NNNCCAGCTGTGATGCCCTG NGG 2 0.2778 Tier II
10 NC_000083.6 17 66568280 + Themis3 NNNCAAGCTATTGTGCCCTG NGG 2 0.2508 Tier II
11 NC_000069.6 3 92408403 - Sprr2i NNNCAAGCTCTGGTGCCCTG NGG 2 0.2174 Tier II
12 NC_000070.6 4 33379765 + Rngtt NNNCGAGCTATGATGCCCTG NAG 2 0.1867 Tier II
13 NC_000080.6 14 29568132 + Cacna2d3 NNNCACGCTCTGATGCCCTG NGG 2 0.1515 Tier II
14 NC_000073.6 7 77021369 + Agbl1 NNNCAAGCTATCATTCCCTG NGG 2 0.0756 Tier II
15 NC_000071.6 5 116475792 - Srrm4 NNNCAAGCTATGATTCCCTC NGG 2 0.0612 Tier II
16 NC_000082.6 16 43355510 + Zbtb20 NNNCAAGCTGTGATGCCCTG NGA 2 0.0386 Tier II
17 NC_000085.6 19 24097980 + Tjp2 NNNCAAGCTAAGATGCCCTG NTG 2 0.0292 Tier II
18 NC_000074.6 8 23787705 - Zmat4 NNNCAAGCTATGATGCACTG NGT 2 0.0075 Tier II
19 NC_000084.6 18 56459905 - Gramd3 NNNCAAGCTATGAGGCCCTG NTG 2 0.0 Tier II
20 NC_000073.6 7 144191479 - Shank2 NNNCAAGCTATGATGCCCTG NTC 2 0.0 Tier II
21 NC_000070.6 4 11667382 - Gm35884 NNNCAAGCCATCATGCCCTG NGG 2 0.4887 Tier III
22 NC_000076.6 10 87242241 - 4930555G07Rik NNNCAAGCCATGACGCCCTG NGG 2 0.2637 Tier III
23 NC_000067.6 1 121092353 - Celrr NNNCAAGCTCTCATGCCCTG NGG 2 0.1765 Tier III
24 NC_000071.6 5 137166488 + Gm40348 NNNCAAGTTATGATGCCCTG NGA 2 0.0608 Tier III
25 NC_000069.6 3 85564562 - Gm3740 NNNCAAGCTATGATTTCCTG NGG 2 0.044 Tier III
26 NC_000082.6 16 43355510 + Gm9968 NNNCAAGCTGTGATGCCCTG NGA 2 0.0386 Tier III
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Other clones with same target sequence:

(none)