Construct: sgRNA BRDN0001148843
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAGAGAAGCACCTACCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK19 (23097)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76503
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
110623291 |
+ |
CDK19 |
NNNGAGAAGCACCTACCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
47987384 |
+ |
MAP4 |
NNNGAAAAGCACTTACCAAG |
NGG |
2 |
0.7 |
Tier I |
| 3 |
NC_000002.12 |
2 |
167251924 |
+ |
XIRP2 |
NNNGAGAACCACCTTCCAAG |
NGG |
2 |
0.1077 |
Tier I |
| 4 |
NC_000011.10 |
11 |
62614816 |
+ |
ROM1 |
NNNGAGAAGCACCTCCCAAG |
NAG |
2 |
0.0707 |
Tier I |
| 5 |
NC_000007.14 |
7 |
101944743 |
- |
CUX1 |
NNNGAAAAGCACCAACCAAG |
NGG |
2 |
0.619 |
Tier II |
| 6 |
NC_000004.12 |
4 |
24872870 |
+ |
CCDC149 |
NNNGATAGGCACCTACCAAG |
NGG |
2 |
0.4889 |
Tier II |
| 7 |
NC_000004.12 |
4 |
24873265 |
+ |
CCDC149 |
NNNGATATGCACCTACCAAG |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000011.10 |
11 |
120702622 |
+ |
GRIK4 |
NNNGAGAAGCAGCAACCAAG |
NGG |
2 |
0.2751 |
Tier II |
| 9 |
NC_000006.12 |
6 |
37680394 |
+ |
MDGA1 |
NNNGAGAAGCACCTCCCAAG |
NGG |
1 |
0.2727 |
Tier II |
| 10 |
NC_000001.11 |
1 |
91875217 |
+ |
TGFBR3 |
NNNGAGAAGCACCTGCCAGG |
NGG |
2 |
0.2438 |
Tier II |
| 11 |
NC_000002.12 |
2 |
240787047 |
+ |
KIF1A |
NNNGAGAAGCACCTGCCAGG |
NGG |
2 |
0.2438 |
Tier II |
| 12 |
NC_000001.11 |
1 |
216200722 |
+ |
USH2A |
NNNGAGAAGCAGCTACAAAG |
NGG |
2 |
0.2074 |
Tier II |
| 13 |
NC_000021.9 |
21 |
32245507 |
+ |
MIS18A |
NNNGAGAAGCACCTTCCAGG |
NGG |
2 |
0.075 |
Tier II |
| 14 |
NC_000022.11 |
22 |
23219220 |
- |
BCR |
NNNGAGAAGCACCTACCCAG |
NTG |
2 |
0.0074 |
Tier II |
| 15 |
NC_000002.12 |
2 |
128474251 |
- |
LOC105373611 |
NNNGACAAGCACCTACAAAG |
NGG |
2 |
0.3182 |
Tier III |
| 16 |
NC_000001.11 |
1 |
216200722 |
+ |
USH2A-AS1 |
NNNGAGAAGCAGCTACAAAG |
NGG |
2 |
0.2074 |
Tier III |
| 17 |
NC_000004.12 |
4 |
116951605 |
+ |
LOC107986306 |
NNNGAGAAGCACATACCATG |
NGG |
2 |
0.2071 |
Tier III |
| 18 |
NC_000020.11 |
20 |
31604856 |
+ |
LOC105372588 |
NNNGAGAAGCATCTTCCAAG |
NGG |
2 |
0.1077 |
Tier III |
| 19 |
NC_000007.14 |
7 |
27380515 |
+ |
LOC105375206 |
NNNGAGAAGCACTGACCAAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
40475641 |
- |
Cdk19 |
NNNGAGAAGCACCTACCAGG |
NGG |
1 |
0.375 |
Tier I |
| 2 |
NC_000068.7 |
2 |
91987037 |
+ |
Creb3l1 |
NNNGAGTAGCACCTACCATG |
NGG |
2 |
0.2356 |
Tier I |
| 3 |
NC_000070.6 |
4 |
47291540 |
+ |
Col15a1 |
NNNGAGAACCACCTACCAAG |
NGG |
1 |
0.5385 |
Tier II |
| 4 |
NC_000077.6 |
11 |
97946037 |
+ |
Plxdc1 |
NNNAAGAACCACCTACCAAG |
NGG |
2 |
0.4846 |
Tier II |
| 5 |
NC_000070.6 |
4 |
76141018 |
+ |
Ptprd |
NNNGATAAGCAGCTACCAAG |
NGG |
2 |
0.2963 |
Tier II |
| 6 |
NC_000077.6 |
11 |
53866821 |
+ |
Slc22a5 |
NNNGAGAAGCAGCTGCCAAG |
NGG |
2 |
0.2889 |
Tier II |
| 7 |
NC_000070.6 |
4 |
58505329 |
+ |
Lpar1 |
NNNGAGAAGAACCCACCAAG |
NGG |
2 |
0.2476 |
Tier II |
| 8 |
NC_000086.7 |
X |
100339086 |
- |
Eda |
NNNCAGCAGCACCTACCAAG |
NGG |
2 |
0.2316 |
Tier II |
| 9 |
NC_000070.6 |
4 |
148995763 |
- |
Pex14 |
NNNGGGAAGCTCCTACCAAG |
NGG |
2 |
0.2215 |
Tier II |
| 10 |
NC_000070.6 |
4 |
123602073 |
- |
Macf1 |
NNNTAGAAGCACCTACCATG |
NGG |
2 |
0.1958 |
Tier II |
| 11 |
NC_000070.6 |
4 |
154109277 |
+ |
Trp73 |
NNNGAGAAGCACCAACCAAG |
NAG |
2 |
0.1605 |
Tier II |
| 12 |
NC_000081.6 |
15 |
76793842 |
+ |
Arhgap39 |
NNNGAGAAGCACCATCCAAG |
NGG |
2 |
0.1238 |
Tier II |
| 13 |
NC_000079.6 |
13 |
109750674 |
- |
Pde4d |
NNNGAGAAGCACCCACCAGG |
NGG |
2 |
0.1071 |
Tier II |
| 14 |
NC_000084.6 |
18 |
40384129 |
- |
Kctd16 |
NNNGAGAAGCACATACCAAG |
NAG |
2 |
0.0997 |
Tier II |
| 15 |
NC_000072.6 |
6 |
48459672 |
- |
Sspo |
NNNGAGAAGCACCTTTCAAG |
NGG |
2 |
0.0615 |
Tier II |
| 16 |
NC_000071.6 |
5 |
45034420 |
+ |
D5Ertd615e |
NNNGCAAAGCACCTACCAAG |
NGG |
2 |
0.5 |
Tier III |
| 17 |
NC_000074.6 |
8 |
55423981 |
+ |
Gm36387 |
NNNGAGAAGCACCTGCCAAT |
NGG |
2 |
0.455 |
Tier III |
| 18 |
NC_000073.6 |
7 |
129768891 |
+ |
Gm33323 |
NNNGATAAGCACCTGCCAAG |
NGG |
2 |
0.4333 |
Tier III |
| 19 |
NC_000069.6 |
3 |
29904293 |
+ |
Mannr |
NNNGAGAAGCACCTACAAAT |
NGG |
2 |
0.3267 |
Tier III |
| 20 |
NC_000077.6 |
11 |
54160474 |
- |
Gm12223 |
NNNCAGCAGCACCTACCAAG |
NGG |
2 |
0.2316 |
Tier III |
| 21 |
NC_000071.6 |
5 |
35336043 |
+ |
Gm31656 |
NNNGAGAAGCACCTGCCAAG |
NAG |
2 |
0.1685 |
Tier III |
| 22 |
NC_000082.6 |
16 |
95777484 |
- |
Gm32130 |
NNNGAGAAGAACCTACCAAG |
NGC |
2 |
0.0193 |
Tier III |
Other clones with same target sequence:
(none)
