Construct: sgRNA BRDN0001148846
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATACTCATTAACGCCATTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CPNE3 (8895)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77554
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
86548372 |
- |
CPNE3 |
NNNACTCATTAACGCCATTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
134189237 |
- |
RYK |
NNNACTCATAAATGCCATTG |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000001.11 |
1 |
176548218 |
- |
PAPPA2 |
NNNACTCATTAACTCCATTT |
NGG |
2 |
0.1867 |
Tier II |
| 4 |
NC_000009.12 |
9 |
84676617 |
+ |
NTRK2 |
NNNACTCATTAACAGCATTG |
NGG |
2 |
0.0375 |
Tier II |
| 5 |
NC_000003.12 |
3 |
59722050 |
- |
CFAP20DC-DT |
NNNACTCATAAACCCCATTG |
NGG |
2 |
0.3673 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
19532462 |
+ |
Cpne3 |
NNNACTCATTAACACCATTC |
NGG |
2 |
0.3214 |
Tier I |
| 2 |
NC_000075.6 |
9 |
51003249 |
- |
Sik2 |
NNNACTCATTATAGCCATTG |
NGG |
2 |
0.1282 |
Tier II |
Other clones with same target sequence:
(none)
