Construct: sgRNA BRDN0001148847

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AACTCCGTAGTTGTCCACGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCR (613)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76907

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000022.11 22 23268439 - BCR NNNTCCGTAGTTGTCCACGA NGG 0 1.0 Tier I
2 NC_000011.10 11 1102525 - MUC2 NNNTCCGAAGTTGTCCACGG NGG 2 0.6118 Tier I
3 NC_000020.11 20 43926901 + TOX2 NNNTCCGCAGTGGTCCACGA NGG 2 0.5 Tier II
4 NC_000001.11 1 183913935 - RGL1 NNNTCCGTAGGTGTCCACGA NGG 1 0.4 Tier II
5 NC_000001.11 1 50048564 + ELAVL4 NNNTCCGTAGTTGTCCTCGA NGT 2 0.0022 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 75135925 - Bcr NNNTCCGTAGTTATCCACAA NGG 2 0.6593 Tier I
2 NC_000068.7 2 135967113 + Plcb4 NNNTCCGTACTTGTCCACGA NGA 2 0.0278 Tier I
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Other clones with same target sequence:

(none)