Construct: sgRNA BRDN0001148851

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGAGTACGCTCTCTTGTCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA2 (6196)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75734

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 166498617 + RPS6KA2 NNNAGTACGCTCTCTTGTCG NGG 0 1.0 Tier I
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 13353883 + Synpr NNNAGTACGCTGTCTTGTCG NTG 2 0.0173 Tier II
Download CSV

Other clones with same target sequence:

(none)