Construct: sgRNA BRDN0001148854

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCAAGAATACTCACATGTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K4 (6416)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76652

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 12054989 - MAP2K4 NNNAGAATACTCACATGTGT NGG 0 1.0 Tier I
2 NC_000009.12 9 20744125 - FOCAD NNNAGAACACTAACATGTGT NGG 2 0.7143 Tier II
3 NC_000006.12 6 126021121 - TRMT11 NNNGGAACACTCACATGTGT NGG 2 0.625 Tier II
4 NC_000001.11 1 64608585 - CACHD1 NNNAGAATACACATATGTGT NGG 2 0.55 Tier II
5 NC_000007.14 7 28818787 - CREB5 NNNAAAATACTCACATGTGG NGG 2 0.1529 Tier II
6 NC_000011.10 11 99532580 + CNTN5 NNNAGAATACTCACATGTTG NGG 2 0.1176 Tier II
7 NC_000010.11 10 96456339 - TLL2 NNNAGAATACACACATGTGT NTG 2 0.0292 Tier II
8 NC_000005.10 5 81513111 + SSBP2 NNNAGAATACTCACATGTAT NTG 2 0.0278 Tier II
9 NC_000018.10 18 76900643 - ZNF236 NNNATAATACTCACATGTGT NGT 2 0.0048 Tier II
10 NC_000001.11 1 215038954 + KCNK2 NNNAGAATACTCACATTTGT NGT 2 0.004 Tier II
11 NC_000018.10 18 4213838 + DLGAP1 NNNAGAATACTCAGATGAGT NGG 2 0.0 Tier II
12 NC_000001.11 1 31472691 - LOC105379772 NNNAGAATTCTCACATGTTT NGG 2 0.4 Tier III
13 NC_000006.12 6 77939996 - LOC105377865 NNNAGAAAACTCACATTTGT NGG 2 0.2 Tier III
14 NC_000007.14 7 53704148 + LINC01446 NNNAGAATACTCATATGTGT NTG 2 0.0286 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 65756272 + Map2k4 NNNGGAGTACTCACATGTGT NGG 2 0.4412 Tier I
2 NC_000068.7 2 155297086 + Pigu NNNAGAATACTCACATCTGT NGA 2 0.0163 Tier I
3 NC_000069.6 3 94471560 + Riiad1 NNNAGAAAACTCACAAGTGT NGG 2 0.7273 Tier II
4 NC_000069.6 3 54799116 - Smad9 NNNAGAAGATTCACATGTGT NGG 2 0.6902 Tier II
5 NC_000080.6 14 15376623 + Lrrc3b NNNAGAATCCTCATATGTGT NGG 2 0.419 Tier II
6 NC_000067.6 1 40758584 + Slc9a2 NNNAGAATACAGACATGTGT NGG 2 0.3333 Tier II
7 NC_000079.6 13 32184600 - Gmds NNNAAAATACTCACAGGTGT NGG 2 0.3 Tier II
8 NC_000069.6 3 64543355 + Vmn2r6 NNNAGAATACTGGCATGTGT NGG 2 0.2899 Tier II
9 NC_000076.6 10 52755363 - Slc35f1 NNNAAAATACTCTCATGTGT NGG 2 0.26 Tier II
10 NC_000067.6 1 46847235 + Slc39a10 NNNAGAATACTCACATGTGT NAG 1 0.2593 Tier II
11 NC_000070.6 4 18008982 - Mmp16 NNNATAATACTCATATGTGT NGG 2 0.22 Tier II
12 NC_000070.6 4 110517816 + Agbl4 NNNAGCATACGCACATGTGT NGG 2 0.1818 Tier II
13 NC_000069.6 3 103580191 - Syt6 NNNGGAATACTCACATGTGT NAG 2 0.162 Tier II
14 NC_000069.6 3 55152116 + Ccdc169 NNNAGAATACTCAAATGTCT NGG 2 0.1569 Tier II
15 NC_000072.6 6 53693613 - Creb5 NNNAAAATACTCACATGTGG NGG 2 0.1529 Tier II
16 NC_000081.6 15 32331434 + Sema5a NNNAGAATAGTCACATTTGT NGG 2 0.0972 Tier II
17 NC_000075.6 9 114981522 + Osbpl10 NNNAGAATAGTCACATGTGG NGG 2 0.0686 Tier II
18 NC_000071.6 5 149744159 + B3glct NNNGGAATACTCACATGTGT NCG 2 0.067 Tier II
19 NC_000073.6 7 82760781 - Efl1 NNNAGAATACACACATGTGT NTG 2 0.0292 Tier II
20 NC_000084.6 18 25581178 - Celf4 NNNAGATTACTCACATGTGT NTG 2 0.017 Tier II
21 NC_000074.6 8 80394694 + Gm30605 NNNAGAATACTCACATGCAT NGG 2 0.3061 Tier III
22 NC_000067.6 1 41904341 + Gm29260 NNNAGAATACTCACAAGGGT NGG 2 0.303 Tier III
23 NC_000071.6 5 149744159 + Gm36141 NNNGGAATACTCACATGTGT NCG 2 0.067 Tier III
24 NC_000079.6 13 50340589 + Gm46385 NNNACAATACTCACATGTGT NGC 2 0.0175 Tier III
25 NC_000084.6 18 25581178 - Gm3227 NNNAGATTACTCACATGTGT NTG 2 0.017 Tier III
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Other clones with same target sequence:

(none)