Construct: sgRNA BRDN0001148855
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCGGTGATATCATATAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TPK1 (27010)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77429
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
144682939 |
+ |
TPK1 |
NNNCGGTGATATCATATAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
37666327 |
+ |
WDR70 |
NNNAGGTGATATCATATAAA |
NGG |
2 |
0.7895 |
Tier II |
| 3 |
NC_000023.11 |
X |
78037169 |
+ |
ATP7A |
NNNTGGTGATATCATATAAA |
NGG |
2 |
0.75 |
Tier II |
| 4 |
NC_000001.11 |
1 |
97726543 |
- |
DPYD |
NNNCTGTGATGTCATATAAG |
NGG |
2 |
0.195 |
Tier II |
| 5 |
NC_000009.12 |
9 |
94780443 |
- |
AOPEP |
NNNCTGTGATATCATATAAG |
NTG |
2 |
0.0117 |
Tier II |
| 6 |
NC_000002.12 |
2 |
14235082 |
- |
LINC00276 |
NNNTGGTGATATCATAAAAG |
NGG |
2 |
0.4267 |
Tier III |
| 7 |
NC_000014.9 |
14 |
46344402 |
+ |
LINC00871 |
NNNCCGTGATATCATATAAG |
NAG |
2 |
0.2037 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
43559973 |
+ |
Tpk1 |
NNNCAGTGAGATCATATAAG |
NGG |
2 |
0.4333 |
Tier I |
| 2 |
NC_000070.6 |
4 |
137242226 |
+ |
Gm31820 |
NNNAGGTTATATCATATAAG |
NGG |
2 |
0.5263 |
Tier III |
Other clones with same target sequence:
(none)
