Construct: sgRNA BRDN0001148856

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GACTGTAGCCAAGCTCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK38L (23012)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76628

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 27297800 + STK38L NNNTGTAGCCAAGCTCACAT NGG 0 1.0 Tier I
2 NC_000005.10 5 138290638 + CDC25C NNNTGTAGCCAAACTCACCT NGG 2 0.191 Tier I
3 NC_000020.11 20 36207987 - EPB41L1 NNNTGTGGCCAAGCTAACAT NGG 2 0.7059 Tier II
4 NC_000010.11 10 110783738 + RBM20 NNNTGTAGTCAAGTTCACAT NGG 2 0.6417 Tier II
5 NC_000006.12 6 36892826 + C6orf89 NNNTGTAGCAAAGCTCACTT NGG 2 0.4667 Tier II
6 NC_000016.10 16 28616742 - SULT1A1 NNNTGTAGCCAAGTTCACAT NAG 2 0.1901 Tier II
7 NC_000018.10 18 59345899 - LMAN1 NNNTGTAGCAAAGCTGACAT NGG 2 0.1333 Tier II
8 NC_000017.11 17 51634623 + CA10 NNNTGTAGCCAAGCTCACAC NGG 1 0.0909 Tier II
9 NC_000007.14 7 50611913 + GRB10 NNNTTTAGCCAAGCTCACCT NGG 2 0.0621 Tier II
10 NC_000009.12 9 81683475 - TLE1 NNNTGTAGCCAAGCTCAAAT NGA 2 0.0374 Tier II
11 NC_000007.14 7 49940029 - ZPBP NNNTGTAGCCAAGCTCTGAT NGG 2 0.0178 Tier II
12 NC_000004.12 4 7684225 - SORCS2 NNNTGTAGCCAAGATCACAT NGT 2 0.0056 Tier II
13 NC_000002.12 2 134502170 + TMEM163 NNNTGTAGCCAAGCTCACAT NAA 2 0.0 Tier II
14 NC_000001.11 1 243956501 - LINC02774 NNNTGTATCCAAGCTCACAT NGG 1 0.625 Tier III
15 NC_000002.12 2 14330495 - LINC00276 NNNTCTGGCCAAGCTCACAT NGG 2 0.5546 Tier III
16 NC_000010.11 10 89925720 + LINC01375 NNNTGTAGCAAAGCTTACAT NGG 2 0.2667 Tier III
17 NC_000016.10 16 28616742 - LOC107984835 NNNTGTAGCCAAGTTCACAT NAG 2 0.1901 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 146758551 + Stk38l NNNTGTGGCCAAGCTCACAC NGG 2 0.0642 Tier I
2 NC_000083.6 17 14329145 - Smoc2 NNNGGTAGCAAAGCTCACAT NGG 2 0.5417 Tier II
3 NC_000070.6 4 107022790 - Ssbp3 NNNTGTGGCCAAGCTCACAA NGG 2 0.3971 Tier II
4 NC_000071.6 5 19621637 + Magi2 NNNTGAAGCCAACCTCACAT NGG 2 0.3649 Tier II
5 NC_000073.6 7 134140568 - Adam12 NNNTGCAGCCAAGATCACAT NGG 2 0.3182 Tier II
6 NC_000074.6 8 16933654 + Csmd1 NNNGGTTGCCAAGCTCACAT NGG 2 0.2734 Tier II
7 NC_000082.6 16 6676499 + Rbfox1 NNNTGTACCCATGCTCACAT NGG 2 0.2051 Tier II
8 NC_000067.6 1 98457847 + Slco6d1 NNNTGTGGCCAAGCTCACAC NGG 2 0.0642 Tier II
9 NC_000079.6 13 41171881 - Sycp2l NNNTCTAGCCAAGCTCACAT NGA 2 0.0546 Tier II
10 NC_000072.6 6 39011635 + Tbxas1 NNNTGTAGCCAAGTGCACAT NGG 2 0.0367 Tier II
11 NC_000079.6 13 104390741 - Adamts6 NNNAGTAGCCAAGCTCACAT NGA 2 0.0331 Tier II
12 NC_000080.6 14 22795693 + Lrmda NNNTGTAGCCAAGCTCACAT NGC 1 0.0222 Tier II
13 NC_000076.6 10 87926228 + Igf1 NNNTGTAGCCAAGCTGACAT NGT 2 0.0025 Tier II
14 NC_000081.6 15 72718294 - Trappc9 NNNGGTAGCCAAGGTCACAT NGG 2 0.0 Tier II
15 NC_000080.6 14 60614465 + Spata13 NNNTGGAGCCAAGGTCACAT NGG 2 0.0 Tier II
16 NC_000075.6 9 42805485 - Grik4 NNNTGTAGCCAAGGTCACAT NGT 2 0.0 Tier II
17 NC_000067.6 1 170521701 - Nos1ap NNNTGTAGCCCAGGTCACAT NGG 2 0.0 Tier II
18 NC_000067.6 1 82488737 + Col4a4 NNNTGTAGCCGAGGTCACAT NGG 2 0.0 Tier II
19 NC_000083.6 17 31868568 - Gm30509 NNNTGTAGACAAGCTCAAAT NGG 2 0.4615 Tier III
20 NC_000070.6 4 107022790 - Gm12786 NNNTGTGGCCAAGCTCACAA NGG 2 0.3971 Tier III
21 NC_000067.6 1 98457847 + Gm3555 NNNTGTGGCCAAGCTCACAC NGG 2 0.0642 Tier III
22 NC_000074.6 8 4641544 + Gm7451 NNNTGTGGCCAAGCTCACAC NGG 2 0.0642 Tier III
23 NC_000080.6 14 22795693 + Gm30926 NNNTGTAGCCAAGCTCACAT NGC 1 0.0222 Tier III
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Other clones with same target sequence:

(none)