Construct: sgRNA BRDN0001148859
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAAGAAAATCTCCATCGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCK (1633)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78057
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
70993914 |
+ |
DCK |
NNNAGAAAATCTCCATCGAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
123977591 |
+ |
FER1L6 |
NNNAGAAAATCTCCAACGAA |
NAG |
2 |
0.2357 |
Tier I |
| 3 |
NC_000008.11 |
8 |
99968222 |
+ |
RGS22 |
NNNAGAAAAACTCCATCAAA |
NGG |
2 |
0.5934 |
Tier II |
| 4 |
NC_000016.10 |
16 |
76486155 |
- |
CNTNAP4 |
NNNAGAAAATCTCCATTGAA |
NGG |
1 |
0.4667 |
Tier II |
| 5 |
NC_000023.11 |
X |
87581700 |
+ |
KLHL4 |
NNNAGAAAAACTCCATCCAA |
NGG |
2 |
0.4082 |
Tier II |
| 6 |
NC_000010.11 |
10 |
82862434 |
- |
NRG3 |
NNNATAAAATCTCCATCTAA |
NGG |
2 |
0.2 |
Tier II |
| 7 |
NC_000015.10 |
15 |
63737164 |
+ |
HERC1 |
NNNAGAAAATCTCAATAGAA |
NGG |
2 |
0.1633 |
Tier II |
| 8 |
NC_000002.12 |
2 |
214730000 |
- |
BARD1 |
NNNAGAAAATTTCCATAGAA |
NGG |
2 |
0.1436 |
Tier II |
| 9 |
NC_000007.14 |
7 |
29844894 |
- |
WIPF3 |
NNNAGAAGATCTGCATCGAA |
NGG |
2 |
0.1 |
Tier II |
| 10 |
NC_000017.11 |
17 |
68587690 |
- |
FAM20A |
NNNTGAAAATCTCCATGGAA |
NGG |
2 |
0.0374 |
Tier II |
| 11 |
NC_000018.10 |
18 |
46811954 |
+ |
PIAS2 |
NNNAGCAAATCTCCATGGAA |
NGG |
2 |
0.0267 |
Tier II |
| 12 |
NC_000009.12 |
9 |
21465038 |
- |
MIR31HG |
NNNAGAAAATATCCATCAAA |
NGG |
2 |
0.5192 |
Tier III |
| 13 |
NC_000002.12 |
2 |
40712096 |
- |
LOC105374497 |
NNNAGAAAAACTCCATCCAA |
NGG |
2 |
0.4082 |
Tier III |
| 14 |
NC_000013.11 |
13 |
63307167 |
+ |
LINC00376 |
NNNAGAAAATCTCCATTCAA |
NGG |
2 |
0.2222 |
Tier III |
| 15 |
NC_000018.10 |
18 |
35398371 |
+ |
LOC105372063 |
NNNAGAAAATCTTCATCGAA |
NTG |
2 |
0.0273 |
Tier III |
| 16 |
NC_000001.11 |
1 |
87237517 |
+ |
LINC02801 |
NNNAGAAAATCTCCATAGAA |
NGC |
2 |
0.0104 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
88765334 |
+ |
Dck |
NNNAGAAGATCTCCATCGAG |
NGG |
2 |
0.5608 |
Tier I |
| 2 |
NC_000068.7 |
2 |
37414169 |
+ |
Rc3h2 |
NNNACAAAATCTCCATCTAA |
NGG |
2 |
0.5238 |
Tier II |
| 3 |
NC_000086.7 |
X |
98805771 |
- |
Ophn1 |
NNNAGAAAATCTCCAACCAA |
NGG |
2 |
0.4329 |
Tier II |
| 4 |
NC_000072.6 |
6 |
72780625 |
+ |
Tcf7l1 |
NNNAGAAAATCTCCAGCGAT |
NGG |
2 |
0.2077 |
Tier II |
| 5 |
NC_000068.7 |
2 |
161583085 |
- |
Ptprt |
NNNAGATAATCTCCATAGAA |
NGG |
2 |
0.2042 |
Tier II |
| 6 |
NC_000070.6 |
4 |
65471635 |
+ |
Astn2 |
NNNAGAAAATCTCCATCACA |
NGG |
2 |
0.1432 |
Tier II |
| 7 |
NC_000067.6 |
1 |
22689241 |
+ |
Rims1 |
NNNAGAAAATCTCCTTCTAA |
NGG |
2 |
0.1333 |
Tier II |
| 8 |
NC_000086.7 |
X |
113770847 |
- |
Dach2 |
NNNAGAAAATCTCCATGGAA |
NGA |
2 |
0.0041 |
Tier II |
| 9 |
NC_000078.6 |
12 |
11874644 |
- |
Gm34237 |
NNNAGAAAATCTCCATAGAA |
NCG |
2 |
0.05 |
Tier III |
| 10 |
NC_000083.6 |
17 |
15232383 |
- |
Gm5091 |
NNNAGAACATCTCCATGGAA |
NGG |
2 |
0.0252 |
Tier III |
Other clones with same target sequence:
(none)
