Construct: sgRNA BRDN0001148860
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATACAATCCCCCAGACCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BRD3 (8019)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76095
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
134045325 |
- |
BRD3 |
NNNCAATCCCCCAGACCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
32977539 |
+ |
BRD2 |
NNNCAATCCCCCAGATCACG |
NTG |
2 |
0.012 |
Tier I |
| 3 |
NC_000015.10 |
15 |
57512877 |
+ |
CGNL1 |
NNNCAAACCTCCAGACCACG |
NGG |
2 |
0.8235 |
Tier II |
| 4 |
NC_000008.11 |
8 |
144665434 |
+ |
ARHGAP39 |
NNNCAATCCCCAAGACCACG |
NGG |
1 |
0.7143 |
Tier II |
| 5 |
NC_000001.11 |
1 |
175984120 |
+ |
COP1 |
NNNTAATCCCCAAGACCACG |
NGG |
2 |
0.5714 |
Tier II |
| 6 |
NC_000002.12 |
2 |
163662517 |
+ |
FIGN |
NNNTAATCCCCAAGACCACG |
NGG |
2 |
0.5714 |
Tier II |
| 7 |
NC_000003.12 |
3 |
133099419 |
- |
TMEM108 |
NNNTAATCCCCAAGACCACG |
NGG |
2 |
0.5714 |
Tier II |
| 8 |
NC_000018.10 |
18 |
33340492 |
+ |
CCDC178 |
NNNTAATCCCCAAGACCACG |
NGG |
2 |
0.5714 |
Tier II |
| 9 |
NC_000019.10 |
19 |
45105079 |
+ |
PPP1R37 |
NNNCAGTGCCCCAGACCACG |
NGG |
2 |
0.4592 |
Tier II |
| 10 |
NC_000011.10 |
11 |
128689817 |
- |
FLI1 |
NNNCGAGCCCCCAGACCACG |
NGG |
2 |
0.4235 |
Tier II |
| 11 |
NC_000006.12 |
6 |
146084591 |
- |
GRM1 |
NNNCAATCCCTCAGACCACA |
NGG |
2 |
0.2885 |
Tier II |
| 12 |
NC_000007.14 |
7 |
151218457 |
+ |
ABCF2 |
NNNAAATCCCCCAGAGCACG |
NGG |
2 |
0.1296 |
Tier II |
| 13 |
NC_000007.14 |
7 |
151218457 |
+ |
ABCF2-H2BK1 |
NNNAAATCCCCCAGAGCACG |
NGG |
2 |
0.1296 |
Tier II |
| 14 |
NC_000018.10 |
18 |
79722591 |
- |
CTDP1 |
NNNCAATCACCCAGACCAGG |
NGG |
2 |
0.1071 |
Tier II |
| 15 |
NC_000002.12 |
2 |
46381380 |
+ |
EPAS1 |
NNNCCATCCCCCAGACCAGG |
NGG |
2 |
0.0625 |
Tier II |
| 16 |
NC_000008.11 |
8 |
25380210 |
+ |
DOCK5 |
NNNCAATCCCCCAGACCACT |
NGC |
2 |
0.0156 |
Tier II |
| 17 |
NC_000002.12 |
2 |
165967823 |
+ |
LOC102724058 |
NNNTAATCCCCAAGACCACG |
NGG |
2 |
0.5714 |
Tier III |
| 18 |
NC_000010.11 |
10 |
24765283 |
- |
LOC105376456 |
NNNTAATCCCCAAGACCACG |
NGG |
2 |
0.5714 |
Tier III |
| 19 |
NC_000005.10 |
5 |
126112953 |
+ |
LOC124901056 |
NNNGAATCCCCAAGACCACG |
NGG |
2 |
0.3571 |
Tier III |
| 20 |
NC_000020.11 |
20 |
55710581 |
+ |
LOC107984001 |
NNNCAATCCCCAAGACCATG |
NGG |
2 |
0.3297 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
34114382 |
- |
Brd2 |
NNNCAATCCTCCAGACCACG |
NTG |
2 |
0.0367 |
Tier I |
| 2 |
NC_000086.7 |
X |
7591675 |
- |
Foxp3 |
NNNCAATCCCCCAAAACACG |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000083.6 |
17 |
81440172 |
+ |
Slc8a1 |
NNNCAATCCACAAGACCACG |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000076.6 |
10 |
115111472 |
+ |
Tph2 |
NNNCAATCCCCAAGACCAAG |
NGG |
2 |
0.3061 |
Tier II |
| 5 |
NC_000083.6 |
17 |
44253449 |
- |
Clic5 |
NNNCAATCCCCCATGCCACG |
NGG |
2 |
0.1733 |
Tier II |
| 6 |
NC_000068.7 |
2 |
80328453 |
- |
Dnajc10 |
NNNCAATCCCCCAGACCACA |
NTG |
2 |
0.0365 |
Tier II |
Other clones with same target sequence:
(none)
