Construct: sgRNA BRDN0001148864

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCGAATGAAGGGCGGCCGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COQ8A (56997)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77322

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 226983012 + COQ8A NNNAATGAAGGGCGGCCGCG NGG 0 1.0 Tier I
2 NC_000005.10 5 1523568 + LPCAT1 NNNACTGAGGGGCGGCCGCG NGG 2 0.3333 Tier II
3 NC_000006.12 6 166824241 + RPS6KA2 NNNAATGAAGGGAGGCAGCG NGG 2 0.1795 Tier II
4 NC_000007.14 7 157579659 - PTPRN2 NNNAATGAAGGGCGGACGCG NGA 2 0.0694 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 8624666 - Tns3 NNNGATGGAGGGCGGCCGCG NGG 2 0.4583 Tier II
2 NC_000073.6 7 45395552 - Snrnp70 NNNTGTGAAGGGCGGCCGCG NGG 2 0.4582 Tier II
3 NC_000079.6 13 58359276 - Kif27 NNNACGGAAGGGCGGCCGCG NGG 2 0.2857 Tier II
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Other clones with same target sequence:

(none)