Construct: sgRNA BRDN0001148869

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AACAGGATGACACAACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IP6K1 (9807)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76137

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 49738322 - IP6K1 NNNAGGATGACACAACAGAA NGG 0 1.0 Tier I
2 NC_000007.14 7 102458861 + ALKBH4 NNNAGGATGTCACAAAAGAA NGG 2 0.8824 Tier II
3 NC_000014.9 14 67392604 - GPHN NNNAGGAAGACACAACAGAA NGG 1 0.8 Tier II
4 NC_000014.9 14 67392604 - PLEK2 NNNAGGAAGACACAACAGAA NGG 1 0.8 Tier II
5 NC_000005.10 5 144314097 + KCTD16 NNNAGAATCACACAACAGAA NGG 2 0.5385 Tier II
6 NC_000006.12 6 138106010 + PERP NNNAGGATTACACAAAAGAA NGG 2 0.5333 Tier II
7 NC_000001.11 1 240928557 + RGS7 NNNAAGATGACACAACAGAT NGG 2 0.52 Tier II
8 NC_000008.11 8 59077580 - TOX NNNAGGAGAACACAACAGAA NGG 2 0.4714 Tier II
9 NC_000003.12 3 96827135 + EPHA6 NNNAGGCTGACACAAAAGAA NGG 2 0.4375 Tier II
10 NC_000016.10 16 53169948 - CHD9 NNNAGGATGGAACAACAGAA NGG 2 0.4167 Tier II
11 NC_000007.14 7 121085574 - CPED1 NNNAGGATGACAAAAAAGAA NGG 2 0.3846 Tier II
12 NC_000019.10 19 56280689 - ZSCAN5A NNNAGGATGACACAACAGGA NGG 1 0.375 Tier II
13 NC_000019.10 19 56280689 - EDDM13 NNNAGGATGACACAACAGGA NGG 1 0.375 Tier II
14 NC_000019.10 19 56280689 - LOC124900420 NNNAGGATGACACAACAGGA NGG 1 0.375 Tier II
15 NC_000012.12 12 18436125 - PIK3C2G NNNAGGATGGCACAGCAGAA NGG 2 0.3611 Tier II
16 NC_000004.12 4 183886345 + STOX2 NNNAGGATTGCACAACAGAA NGG 2 0.2963 Tier II
17 NC_000020.11 20 42264453 - PTPRT NNNAGGCTGACACTACAGAA NGG 2 0.2333 Tier II
18 NC_000004.12 4 6000650 - C4orf50 NNNAGGATGACAAAACAGAT NGG 2 0.2308 Tier II
19 NC_000006.12 6 79011490 + PHIP NNNAAGATGAGACAACAGAA NGG 2 0.2167 Tier II
20 NC_000001.11 1 53655000 + GLIS1 NNNGGGATGACTCAACAGAA NGG 2 0.2083 Tier II
21 NC_000003.12 3 46084133 - XCR1 NNNAGGATGACAATACAGAA NGG 2 0.2051 Tier II
22 NC_000009.12 9 13129533 + MPDZ NNNAGGATGACACATAAGAA NGG 2 0.2 Tier II
23 NC_000011.10 11 1307642 + TOLLIP NNNAGGAGGACACAACAGCA NGG 2 0.1517 Tier II
24 NC_000009.12 9 81625314 + TLE1 NNNAGGATGACAGAACAGAA NGG 1 0.1364 Tier II
25 NC_000003.12 3 105538601 - ALCAM NNNAGGTTGACACAACAGAA NAG 2 0.1134 Tier II
26 NC_000004.12 4 171923506 - GALNTL6 NNNAGGCTGAGACAACAGAA NGG 2 0.1094 Tier II
27 NC_000017.11 17 5333764 - RABEP1 NNNAGGATGAAAGAACAGAA NGG 2 0.1023 Tier II
28 NC_000001.11 1 167462807 - CD247 NNNAGGATGACACAGGAGAA NGG 2 0.1 Tier II
29 NC_000003.12 3 143802226 - SLC9A9 NNNATGATGACACAATAGAA NGG 2 0.0923 Tier II
30 NC_000016.10 16 54095836 + FTO NNNAGGATGACAGAGCAGAA NGG 2 0.0886 Tier II
31 NC_000018.10 18 69003532 + CCDC102B NNNAGGATGACAGAGCAGAA NGG 2 0.0886 Tier II
32 NC_000013.11 13 24067005 - SPATA13 NNNAGGATGGCACAAGAGAA NGG 2 0.0855 Tier II
33 NC_000006.12 6 16291163 - GMPR NNNTGGATGACACAACTGAA NGG 2 0.0848 Tier II
34 NC_000011.10 11 104016260 - PDGFD NNNAGGATGGCAGAACAGAA NGG 2 0.0758 Tier II
35 NC_000002.12 2 232902169 + NGEF NNNAGGATGACTCAACAGCA NGG 2 0.069 Tier II
36 NC_000012.12 12 18702529 - PIK3C2G NNNAGGATGACAAAAGAGAA NGG 2 0.0592 Tier II
37 NC_000012.12 12 18702529 - PLCZ1 NNNAGGATGACAAAAGAGAA NGG 2 0.0592 Tier II
38 NC_000023.11 X 130860064 - ENOX2 NNNAGGCTGACACAACAGAA NCG 2 0.0469 Tier II
39 NC_000023.11 X 28893803 + IL1RAPL1 NNNATGATGACAGAACAGAA NGG 2 0.0409 Tier II
40 NC_000012.12 12 53027242 - EIF4B NNNAGGATGAGACAAGAGAA NGG 2 0.0385 Tier II
41 NC_000005.10 5 142916866 - ARHGAP26 NNNAGGATGACACAACACAA NGA 2 0.0331 Tier II
42 NC_000003.12 3 64649476 - ADAMTS9 NNNAGGATGACACAACAGTA NTG 2 0.021 Tier II
43 NC_000018.10 18 55230944 + TCF4 NNNAGGATGACACCACAGAA NGA 2 0.0149 Tier II
44 NC_000008.11 8 103920341 - RIMS2 NNNAGGAAGACACAACAGAA NGT 2 0.0129 Tier II
45 NC_000001.11 1 111591741 - RAP1A NNNAGGATGACAGAACAGAA NGA 2 0.0095 Tier II
46 NC_000021.9 21 46669889 - LOC124905058 NNNTGGATGACACAACAGAG NGG 2 0.4866 Tier III
47 NC_000002.12 2 220240512 - LOC105373893 NNNGGGATGACACAACAAAA NGG 2 0.4327 Tier III
48 NC_000012.12 12 89804754 + LOC107984543 NNNAGGATAACACAACATAA NGG 2 0.4286 Tier III
49 NC_000012.12 12 18436125 - LOC124902891 NNNAGGATGGCACAGCAGAA NGG 2 0.3611 Tier III
50 NC_000004.12 4 174366035 - LOC105377547 NNNAGGATGACACAAAAGAA NAG 2 0.2593 Tier III
51 NC_000006.12 6 79011490 + LOC124901346 NNNAAGATGAGACAACAGAA NGG 2 0.2167 Tier III
52 NC_000001.11 1 167462807 - LOC101928512 NNNAGGATGACACAGGAGAA NGG 2 0.1 Tier III
53 NC_000013.11 13 48153691 - LOC105370198 NNNAGGATGACAAATCAGAA NGG 2 0.0769 Tier III
54 NC_000013.11 13 48155321 + LOC105370198 NNNAGGATGACAAATCAGAA NGG 2 0.0769 Tier III
55 NC_000001.11 1 163050300 + LOC107985223 NNNAGAATGACACAACAGAA NGA 2 0.0694 Tier III
56 NC_000004.12 4 154061211 - LOC101927947 NNNAGGATGACAGAATAGAA NGG 2 0.042 Tier III
57 NC_000023.11 X 129124902 - LOC124905213 NNNAGGATGATACAACAGAA NGA 2 0.0214 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 29164531 - Setx NNNACGATGACACAACTGAA NGG 2 0.1048 Tier I
2 NC_000072.6 6 23025982 + Ptprz1 NNNAAGATGAAACAACAGAA NGG 2 0.65 Tier II
3 NC_000078.6 12 74226458 + Syt16 NNNAGAATAACACAACAGAA NGG 2 0.6429 Tier II
4 NC_000075.6 9 101016262 + Pccb NNNTGAATGACACAACAGAA NGG 2 0.6364 Tier II
5 NC_000068.7 2 166599378 + Prex1 NNNAAGATGACACAGCAGAA NGG 2 0.5633 Tier II
6 NC_000068.7 2 146493773 + Ralgapa2 NNNAGGATGACACAGCAGAG NGG 2 0.4971 Tier II
7 NC_000078.6 12 56718346 + Slc25a21 NNNAGGATAAAACAACAGAA NGG 2 0.4821 Tier II
8 NC_000082.6 16 94295259 + Hlcs NNNAGGATAAAACAACAGAA NGG 2 0.4821 Tier II
9 NC_000068.7 2 24420893 - Pax8 NNNAGGAAGACACAACAGAT NGG 2 0.48 Tier II
10 NC_000077.6 11 84066218 - Dusp14 NNNGGGGTGACACAACAGAA NGG 2 0.4412 Tier II
11 NC_000067.6 1 75218961 + Tuba4a NNNAGGAGGACACTACAGAA NGG 2 0.3911 Tier II
12 NC_000081.6 15 22825906 - Cdh18 NNNAGGATGACAAAACAGAA NGG 1 0.3846 Tier II
13 NC_000084.6 18 47329747 - Sema6a NNNAGGGTGACACAACACAA NGG 2 0.3361 Tier II
14 NC_000078.6 12 76130163 - Esr2 NNNACGATGACAAAACAGAA NGG 2 0.3022 Tier II
15 NC_000072.6 6 115210575 - Syn2 NNNAGGAGGACACAACAGGA NGG 2 0.275 Tier II
16 NC_000076.6 10 117091505 + Frs2 NNNAGGATGACACACAAGAA NGG 2 0.2727 Tier II
17 NC_000082.6 16 21874801 - Map3k13 NNNAGAATGACACAACAGAA NAG 2 0.2593 Tier II
18 NC_000075.6 9 15961039 + Fat3 NNNAAGATGACACACCAGAA NGG 2 0.2364 Tier II
19 NC_000068.7 2 179840704 - Cdh4 NNNAGGGTGATACAACAGAA NGG 2 0.2172 Tier II
20 NC_000071.6 5 130054365 - Crcp NNNAGGATGACACCAAAGAA NGG 2 0.2143 Tier II
21 NC_000081.6 15 97330036 - Pced1b NNNAGCATGATACAACAGAA NGG 2 0.2098 Tier II
22 NC_000078.6 12 76939167 + Max NNNAGGATGACACAATAGAT NGG 2 0.1846 Tier II
23 NC_000070.6 4 72199114 + Tle1 NNNAGGAGGAGACAACAGAA NGG 2 0.1833 Tier II
24 NC_000082.6 16 4478423 - Srl NNNAGGAGGAGACAACAGAA NGG 2 0.1833 Tier II
25 NC_000072.6 6 67336441 + Il12rb2 NNNAGGATGACTCAACAGGA NGG 2 0.125 Tier II
26 NC_000068.7 2 17447188 - Nebl NNNAGGAAGACACAAGAGAA NGG 2 0.1231 Tier II
27 NC_000074.6 8 117616045 + Plcg2 NNNAGGAAGACAGAACAGAA NGG 2 0.1091 Tier II
28 NC_000074.6 8 23421363 - Sfrp1 NNNAGGATGCTACAACAGAA NGG 2 0.1026 Tier II
29 NC_000086.7 X 142811480 - Tmem164 NNNAGGATGAAAGAACAGAA NGG 2 0.1023 Tier II
30 NC_000082.6 16 35100722 + Hacd2 NNNAGGATGACAGAGCAGAA NGG 2 0.0886 Tier II
31 NC_000082.6 16 45944950 + Phldb2 NNNAGGATGACACAACTCAA NGG 2 0.0635 Tier II
32 NC_000074.6 8 87897893 + Zfp423 NNNAGGAAGACACAACAGAA NGA 2 0.0556 Tier II
33 NC_000080.6 14 55866194 - Nynrin NNNAGGATGACACAACAGAG NGA 2 0.0531 Tier II
34 NC_000075.6 9 35047765 - St3gal4 NNNAGGATGACACAACTGGA NGG 2 0.05 Tier II
35 NC_000070.6 4 111366308 - Agbl4 NNNAGGATGAGAGAACAGAA NGG 2 0.0341 Tier II
36 NC_000074.6 8 16723012 - Csmd1 NNNAGGATGACACAACAGAT NTG 2 0.0234 Tier II
37 NC_000072.6 6 34787671 - Agbl3 NNNAGGATGACAGAACTGAA NGG 2 0.0182 Tier II
38 NC_000069.6 3 88804596 - 5830417I10Rik NNNAGAATGACACAAAAGAA NGG 2 1.0 Tier III
39 NC_000074.6 8 121215553 + Gm33739 NNNTGGATGTCACAACAGAA NGG 2 0.5615 Tier III
40 NC_000068.7 2 51439514 + Gm32434 NNNAGGATGCAACAACAGAA NGG 2 0.25 Tier III
41 NC_000079.6 13 31335089 + G630018N14Rik NNNAGGATGACTCAACAGAA NAG 2 0.0864 Tier III
42 NC_000079.6 13 15917990 + B230303A05Rik NNNAGGAAGACACAACAGAA NTG 2 0.0312 Tier III
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Other clones with same target sequence:

(none)