Construct: sgRNA BRDN0001148870

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTCAAACTTTCCATATCGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK5 (26289)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76554

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 77293822 + AK5 NNNCAAACTTTCCATATCGG NGG 0 1.0 Tier I
2 NC_000015.10 15 39163075 - LOC105370777 NNNAAAACTTTCCATATCTG NGG 2 0.5614 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 152656008 - Ak5 NNNCAAACTTTCCATATCGG NGG 0 1.0 Tier I
2 NC_000078.6 12 79618869 - Rad51b NNNCAAACTTTCCATATCCA NGG 2 0.4203 Tier II
3 NC_000070.6 4 97916801 - Nfia NNNCAATCTTTCCATATCAG NGG 2 0.3125 Tier II
4 NC_000074.6 8 44947675 - Fat1 NNNCAAACTTTCCATATTGG NAG 2 0.1667 Tier II
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Other clones with same target sequence:

(none)