Construct: sgRNA BRDN0001148884
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGAGGTTTAAGGTCTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK2 (1017)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77194
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
55968840 |
- |
CDK2 |
NNNGAGGTTTAAGGTCTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
6386022 |
- |
ACOT7 |
NNNGAGGTTTAAGGTCCTGG |
NGG |
2 |
0.4538 |
Tier II |
| 3 |
NC_000023.11 |
X |
31735398 |
- |
DMD |
NNNGAGGTTTAGGGTCTAGG |
NGG |
2 |
0.3889 |
Tier II |
| 4 |
NC_000018.10 |
18 |
35243580 |
+ |
ZNF397 |
NNNGAGGTTTCAGGTCTAGG |
NGG |
2 |
0.2154 |
Tier II |
| 5 |
NC_000002.12 |
2 |
184311384 |
+ |
LOC102724340 |
NNNGAAGTTTAAGGTCTTGG |
NGG |
2 |
0.6429 |
Tier III |
| 6 |
NC_000002.12 |
2 |
184311384 |
+ |
LOC105373776 |
NNNGAAGTTTAAGGTCTTGG |
NGG |
2 |
0.6429 |
Tier III |
| 7 |
NC_000015.10 |
15 |
69603013 |
+ |
PCAT29 |
NNNGAGCTTTAAGGTCTCAG |
NGG |
2 |
0.4911 |
Tier III |
| 8 |
NC_000001.11 |
1 |
144836225 |
+ |
LOC105371217 |
NNNGAGTTTTAAGGTCACGG |
NGG |
2 |
0.3048 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
52401135 |
- |
Nepn |
NNNGATGTTTCAGGTCTCGG |
NGG |
2 |
0.2667 |
Tier I |
| 2 |
NC_000071.6 |
5 |
34891521 |
- |
Htt |
NNNGAGGCTTCAGGTCTCGG |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000080.6 |
14 |
67008450 |
- |
Bnip3l |
NNNGAGGTTTAGGGTCGCGG |
NGG |
2 |
0.085 |
Tier II |
| 4 |
NC_000071.6 |
5 |
121811833 |
- |
Atxn2 |
NNNGAGGTTTAAGGTCTCAG |
NTG |
2 |
0.0278 |
Tier II |
| 5 |
NC_000068.7 |
2 |
158084642 |
+ |
2010009K17Rik |
NNNAAGGTTTGAGGTCTCGG |
NGG |
2 |
0.585 |
Tier III |
| 6 |
NC_000073.6 |
7 |
31209830 |
+ |
Gm37292 |
NNNGAGGTTTAAGGTCTGGA |
NGG |
2 |
0.125 |
Tier III |
Other clones with same target sequence:
(none)
