Construct: sgRNA BRDN0001148891
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCTCCAAAGAACCCCCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WNK4 (65266)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75658
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
42781016 |
+ |
WNK4 |
NNNTCCAAAGAACCCCCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
87530822 |
+ |
DAPK1 |
NNNTCCAAAGAAACCCCCAA |
NGG |
2 |
0.2747 |
Tier II |
| 3 |
NC_000022.11 |
22 |
19784164 |
+ |
GNB1L |
NNNTCTAAAGAACCCCCGGA |
NGG |
2 |
0.1238 |
Tier II |
| 4 |
NC_000003.12 |
3 |
43377750 |
+ |
ANO10 |
NNNTCCAAAGAAACCCCCGC |
NGG |
2 |
0.0874 |
Tier II |
| 5 |
NC_000014.9 |
14 |
76411701 |
- |
ESRRB |
NNNTCCAAAGAACCCTCCGA |
NGT |
2 |
0.005 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
101260983 |
+ |
Wnk4 |
NNNTCTAAAGAACCCCCCGA |
NGG |
1 |
0.9286 |
Tier I |
| 2 |
NC_000071.6 |
5 |
135049888 |
- |
Stx1a |
NNNTCCAAAGATGCCCCCGA |
NGG |
2 |
0.0455 |
Tier I |
| 3 |
NC_000075.6 |
9 |
14607368 |
+ |
Amotl1 |
NNNTCCACAGAACCCCCAGA |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000074.6 |
8 |
122589437 |
+ |
Galns |
NNNCCCAAAGAACACCCCGA |
NGG |
2 |
0.2265 |
Tier II |
| 5 |
NC_000074.6 |
8 |
122589437 |
+ |
Gm34962 |
NNNCCCAAAGAACACCCCGA |
NGG |
2 |
0.2265 |
Tier III |
Other clones with same target sequence:
(none)
