Construct: sgRNA BRDN0001148893
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGCTTAATATAAGGAGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ICK (22858)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77413
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
53013873 |
+ |
CILK1 |
NNNGCTTAATATAAGGAGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
62747796 |
+ |
EHBP1 |
NNNACCTAATATAAGGAGGT |
NGG |
2 |
0.8182 |
Tier II |
| 3 |
NC_000012.12 |
12 |
44240191 |
- |
TMEM117 |
NNNGCAAAATATAAGGAGGT |
NGG |
2 |
0.7583 |
Tier II |
| 4 |
NC_000006.12 |
6 |
26646358 |
+ |
ZNF322 |
NNNGCTAAAAATAAGGAGGT |
NGG |
2 |
0.75 |
Tier II |
| 5 |
NC_000002.12 |
2 |
11263956 |
+ |
ROCK2 |
NNNACTCAATATAAGGAGGT |
NGG |
2 |
0.6188 |
Tier II |
| 6 |
NC_000014.9 |
14 |
57965754 |
+ |
SLC35F4 |
NNNGCTTAATGTGAGGAGGT |
NGG |
2 |
0.4239 |
Tier II |
| 7 |
NC_000002.12 |
2 |
170021556 |
- |
UBR3 |
NNNGGTTAGTATAAGGAGGT |
NGG |
2 |
0.4 |
Tier II |
| 8 |
NC_000022.11 |
22 |
17745682 |
+ |
BID |
NNNGCTTAAAATAAGGAGCT |
NGG |
2 |
0.3842 |
Tier II |
| 9 |
NC_000007.14 |
7 |
96189532 |
- |
SLC25A13 |
NNNGCTTAATAAAAGGAGGT |
NAG |
2 |
0.2074 |
Tier II |
| 10 |
NC_000004.12 |
4 |
120738157 |
- |
PRDM5 |
NNNGCTTAAGATTAGGAGGT |
NGG |
2 |
0.15 |
Tier II |
| 11 |
NC_000013.11 |
13 |
94141339 |
+ |
GPC6 |
NNNGCTTAATATAAGGAAGT |
NTG |
2 |
0.027 |
Tier II |
| 12 |
NC_000008.11 |
8 |
12066418 |
+ |
DEFB130B |
NNNGCTTAATTTAAGGAGGT |
NTG |
2 |
0.012 |
Tier II |
| 13 |
NC_000008.11 |
8 |
12312987 |
+ |
DEFB130A |
NNNGCTTAATTTAAGGAGGT |
NTG |
2 |
0.012 |
Tier II |
| 14 |
NC_000013.11 |
13 |
29571906 |
- |
SLC7A1 |
NNNGCTTAATACAAGCAGGT |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000007.14 |
7 |
91357128 |
+ |
LINC02932 |
NNNGCATAGTATAAGGAGGT |
NGG |
2 |
0.5778 |
Tier III |
| 16 |
NC_000006.12 |
6 |
29929537 |
+ |
HLA-K |
NNNGCATAATATGAGGAGGT |
NGG |
2 |
0.5652 |
Tier III |
| 17 |
NC_000006.12 |
6 |
29929537 |
+ |
LOC124901298 |
NNNGCATAATATGAGGAGGT |
NGG |
2 |
0.5652 |
Tier III |
| 18 |
NC_000014.9 |
14 |
81740696 |
+ |
LINC02311 |
NNNGCTTAAGAAAAGGAGGT |
NGG |
2 |
0.4 |
Tier III |
| 19 |
NC_000014.9 |
14 |
81740696 |
+ |
LOC107984704 |
NNNGCTTAAGAAAAGGAGGT |
NGG |
2 |
0.4 |
Tier III |
| 20 |
NC_000004.12 |
4 |
9427655 |
- |
DEFB130D |
NNNGCTTAATTTAAGGAGGT |
NTG |
2 |
0.012 |
Tier III |
| 21 |
NC_000003.12 |
3 |
109791740 |
- |
LOC124906267 |
NNNGCTTAATATAAGGAAGT |
NGT |
2 |
0.0112 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
75517621 |
- |
Tmod3 |
NNNGCATAATATAAAGAGGT |
NGG |
2 |
0.8157 |
Tier II |
| 2 |
NC_000077.6 |
11 |
33571695 |
+ |
Gabrp |
NNNGCTTAAAACAAGGAGGT |
NGG |
2 |
0.812 |
Tier II |
| 3 |
NC_000081.6 |
15 |
20839479 |
- |
Cdh12 |
NNNGTTTAATATAAAGAGGT |
NGG |
2 |
0.5989 |
Tier II |
| 4 |
NC_000078.6 |
12 |
82378312 |
- |
Sipa1l1 |
NNNCCTTAATATAAGGAGAT |
NGG |
2 |
0.3782 |
Tier II |
| 5 |
NC_000077.6 |
11 |
26504404 |
+ |
Vrk2 |
NNNCCTTAGTATAAGGAGGT |
NGG |
2 |
0.3529 |
Tier II |
| 6 |
NC_000067.6 |
1 |
58405155 |
- |
Bzw1 |
NNNGCTTGATATAGGGAGGT |
NGG |
2 |
0.3422 |
Tier II |
| 7 |
NC_000077.6 |
11 |
105774873 |
- |
Tanc2 |
NNNGCTTGATATAGGGAGGT |
NGG |
2 |
0.3422 |
Tier II |
| 8 |
NC_000084.6 |
18 |
15395655 |
- |
Aqp4 |
NNNGTTTAAGATAAGGAGGT |
NGG |
2 |
0.3182 |
Tier II |
| 9 |
NC_000076.6 |
10 |
49192365 |
- |
Grik2 |
NNNTTTTAATATAAGGAGGT |
NGG |
2 |
0.2314 |
Tier II |
| 10 |
NC_000077.6 |
11 |
97983611 |
- |
Plxdc1 |
NNNGCTTTATATCAGGAGGT |
NGG |
2 |
0.0902 |
Tier II |
| 11 |
NC_000070.6 |
4 |
138251575 |
+ |
Sh2d5 |
NNNGCTAAATATAAGGAGGC |
NGG |
2 |
0.0795 |
Tier II |
| 12 |
NC_000067.6 |
1 |
62183079 |
+ |
Pard3b |
NNNGCTTAATTTAAGGAGGG |
NGG |
2 |
0.0543 |
Tier II |
| 13 |
NC_000077.6 |
11 |
105774873 |
- |
Gm11652 |
NNNGCTTGATATAGGGAGGT |
NGG |
2 |
0.3422 |
Tier III |
| 14 |
NC_000084.6 |
18 |
15395655 |
- |
A830021F12Rik |
NNNGTTTAAGATAAGGAGGT |
NGG |
2 |
0.3182 |
Tier III |
Other clones with same target sequence:
(none)
