Construct: sgRNA BRDN0001148904
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACTACACCAAGACAAGTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FRK (2444)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76243
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
115968616 |
- |
FRK |
NNNTACACCAAGACAAGTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
126796343 |
+ |
LMNB1 |
NNNTACACCTAGACAAATGA |
NGG |
2 |
0.8235 |
Tier II |
| 3 |
NC_000001.11 |
1 |
202481284 |
- |
PPP1R12B |
NNNTACACCAACACAAGTGG |
NGG |
2 |
0.4048 |
Tier II |
| 4 |
NC_000016.10 |
16 |
83024891 |
+ |
CDH13 |
NNNTTCACCAAAACAAGTGA |
NGG |
2 |
0.3394 |
Tier II |
| 5 |
NC_000001.11 |
1 |
9874241 |
- |
CTNNBIP1 |
NNNTACACCAAGACAAAGGA |
NGG |
2 |
0.3111 |
Tier II |
| 6 |
NC_000006.12 |
6 |
35951911 |
- |
SLC26A8 |
NNNTACCCCCAGACAAGTGA |
NGG |
2 |
0.1458 |
Tier II |
| 7 |
NC_000006.12 |
6 |
64127591 |
- |
EYS |
NNNTACATCAAGACAAGTGA |
NTG |
2 |
0.0341 |
Tier II |
| 8 |
NC_000005.10 |
5 |
109529953 |
- |
LOC105379117 |
NNNTGCATCAAGACAAGTGA |
NGG |
2 |
0.63 |
Tier III |
| 9 |
NC_000024.10 |
Y |
26298420 |
+ |
PPP1R12BP1 |
NNNTACACCAACACAAGTGG |
NGG |
2 |
0.4048 |
Tier III |
| 10 |
NC_000012.12 |
12 |
73842808 |
- |
LINC02445 |
NNNTACACAAAGACCAGTGA |
NGG |
2 |
0.2338 |
Tier III |
| 11 |
NC_000010.11 |
10 |
120970277 |
- |
LOC105378519 |
NNNTACACCATGACCAGTGA |
NGG |
2 |
0.0839 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
34584022 |
+ |
Frk |
NNNTACACCACAACAAGTGA |
NGG |
2 |
0.3733 |
Tier I |
| 2 |
NC_000068.7 |
2 |
60257095 |
- |
Cd302 |
NNNTACACCAAGACAGGTGA |
NTG |
2 |
0.0075 |
Tier I |
| 3 |
NC_000067.6 |
1 |
10699333 |
+ |
Cpa6 |
NNNTAAACCAAGACAAATGA |
NGG |
2 |
0.8667 |
Tier II |
| 4 |
NC_000071.6 |
5 |
31509725 |
- |
Gpn1 |
NNNTACACGAAGACAAGTGA |
NGG |
1 |
0.619 |
Tier II |
| 5 |
NC_000071.6 |
5 |
96213123 |
+ |
Mrpl1 |
NNNAACACCAAAACAAGTGA |
NGG |
2 |
0.4444 |
Tier II |
| 6 |
NC_000070.6 |
4 |
135215994 |
+ |
Clic4 |
NNNTATCCCAAGACAAGTGA |
NGG |
2 |
0.4062 |
Tier II |
| 7 |
NC_000084.6 |
18 |
25115098 |
- |
Fhod3 |
NNNTACACAAAGACAAGTGC |
NGG |
2 |
0.1948 |
Tier II |
| 8 |
NC_000068.7 |
2 |
140468848 |
- |
Macrod2 |
NNNTACACCAAGACAAGTAA |
NAG |
2 |
0.1852 |
Tier II |
| 9 |
NC_000068.7 |
2 |
27111360 |
- |
Fam163b |
NNNTACACCCAGACAAGTGA |
NAG |
2 |
0.0864 |
Tier II |
| 10 |
NC_000072.6 |
6 |
33853926 |
+ |
Exoc4 |
NNNTGCACCAAGACAAGTGA |
NGA |
2 |
0.05 |
Tier II |
| 11 |
NC_000079.6 |
13 |
67348113 |
+ |
Zfp953 |
NNNTACACCAAGACAAGTGA |
NTT |
2 |
0.0 |
Tier II |
| 12 |
NC_000079.6 |
13 |
10531747 |
- |
Gm26861 |
NNNTACACCAAGACAAGTGA |
NTT |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
