Construct: sgRNA BRDN0001148905
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCATCCCGACATGTATGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IKBKE (9641)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77769
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
206476693 |
+ |
IKBKE |
NNNATCCCGACATGTATGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
113323108 |
- |
CFAP44 |
NNNATCCCCACATGTATGGG |
NGG |
2 |
0.2019 |
Tier II |
| 3 |
NC_000015.10 |
15 |
80832567 |
- |
CEMIP |
NNNATCCTGACATGTATGAG |
NGT |
2 |
0.0141 |
Tier II |
| 4 |
NC_000019.10 |
19 |
27987489 |
+ |
LINC02987 |
NNNATCCTGACATATATGAG |
NGG |
2 |
0.6563 |
Tier III |
| 5 |
NC_000019.10 |
19 |
27987489 |
+ |
LOC105372346 |
NNNATCCTGACATATATGAG |
NGG |
2 |
0.6563 |
Tier III |
| 6 |
NC_000003.12 |
3 |
113323108 |
- |
LOC127898559 |
NNNATCCCCACATGTATGGG |
NGG |
2 |
0.2019 |
Tier III |
| 7 |
NC_000006.12 |
6 |
156000909 |
+ |
LOC101928923 |
NNNATCCTGACATGTATGAG |
NTG |
2 |
0.0341 |
Tier III |
| 8 |
NC_000006.12 |
6 |
156000909 |
+ |
LOC124901442 |
NNNATCCTGACATGTATGAG |
NTG |
2 |
0.0341 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
121570777 |
- |
Tbk1 |
NNNATCCGGACATGTATGAA |
NGG |
2 |
0.6027 |
Tier I |
| 2 |
NC_000068.7 |
2 |
74567738 |
+ |
Lnpk |
NNNATCCAGACATGTATAAG |
NGG |
2 |
0.45 |
Tier II |
| 3 |
NC_000067.6 |
1 |
170080587 |
+ |
Ddr2 |
NNNATCTCGACATGTATCAG |
NGG |
2 |
0.3869 |
Tier II |
| 4 |
NC_000082.6 |
16 |
97873025 |
- |
C2cd2 |
NNNGTCCCTACATGTATGAG |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000067.6 |
1 |
51832712 |
+ |
Myo1b |
NNNATCCTGACCTGTATGAG |
NGG |
2 |
0.2303 |
Tier II |
| 6 |
NC_000068.7 |
2 |
167635575 |
- |
Gm31852 |
NNNGTCCTGACATGTATGAG |
NGG |
2 |
0.5469 |
Tier III |
Other clones with same target sequence:
(none)
