Construct: sgRNA BRDN0001148914
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCGGAAGGAGACCACAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPKC (80271)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77653
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
40725388 |
- |
ITPKC |
NNNCGGAAGGAGACCACAAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
122959472 |
+ |
JHY |
NNNAGGAAGGAGACCAAAAA |
NGG |
2 |
0.393 |
Tier II |
| 3 |
NC_000006.12 |
6 |
87316715 |
- |
GJB7 |
NNNCAGCAGGAGACCACAAA |
NGG |
2 |
0.3792 |
Tier II |
| 4 |
NC_000002.12 |
2 |
44708584 |
+ |
CAMKMT |
NNNGGGAAGGAGATCACAAA |
NGG |
2 |
0.3667 |
Tier II |
| 5 |
NC_000009.12 |
9 |
96345418 |
+ |
SLC35D2 |
NNNGGGAGGGAGACCACAAA |
NGG |
2 |
0.3667 |
Tier II |
| 6 |
NC_000023.11 |
X |
6149186 |
- |
NLGN4X |
NNNTGGATGGAGACCACAAA |
NGG |
2 |
0.3429 |
Tier II |
| 7 |
NC_000012.12 |
12 |
13601730 |
+ |
GRIN2B |
NNNGGGAAGGGGACCACAAA |
NGG |
2 |
0.325 |
Tier II |
| 8 |
NC_000018.10 |
18 |
48772072 |
+ |
CTIF |
NNNAGGAAGGAGACCACAGA |
NGG |
2 |
0.3158 |
Tier II |
| 9 |
NC_000020.11 |
20 |
53968225 |
+ |
BCAS1 |
NNNGGGATGGAGACCACAAA |
NGG |
2 |
0.2143 |
Tier II |
| 10 |
NC_000012.12 |
12 |
86601287 |
+ |
MGAT4C |
NNNCGGATGGAGACCACAAC |
NGG |
2 |
0.0974 |
Tier II |
| 11 |
NC_000016.10 |
16 |
70716815 |
+ |
VAC14 |
NNNCGGAAGGAGGCCACAAA |
NGA |
2 |
0.0453 |
Tier II |
| 12 |
NC_000005.10 |
5 |
125289139 |
+ |
LOC101927421 |
NNNAGGTAGGAGACCACAAA |
NGG |
2 |
0.3684 |
Tier III |
| 13 |
NC_000009.12 |
9 |
96345418 |
+ |
SLC35D2-HSD17B3 |
NNNGGGAGGGAGACCACAAA |
NGG |
2 |
0.3667 |
Tier III |
| 14 |
NC_000012.12 |
12 |
13601730 |
+ |
LOC105369668 |
NNNGGGAAGGGGACCACAAA |
NGG |
2 |
0.325 |
Tier III |
| 15 |
NC_000018.10 |
18 |
48772072 |
+ |
LOC107985147 |
NNNAGGAAGGAGACCACAGA |
NGG |
2 |
0.3158 |
Tier III |
| 16 |
NC_000012.12 |
12 |
129965833 |
+ |
LOC105370076 |
NNNGGGAAGGAGACCACACA |
NGG |
2 |
0.1034 |
Tier III |
| 17 |
NC_000004.12 |
4 |
19887913 |
- |
LOC105374511 |
NNNCGGAAGGAGACGATAAA |
NGG |
2 |
0.0233 |
Tier III |
| 18 |
NC_000004.12 |
4 |
14678991 |
+ |
LINC00504 |
NNNCGGAAGGAGACCCCAAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
27219655 |
+ |
Itpkc |
NNNTGGAAGGAGACCACGAA |
NGG |
2 |
0.32 |
Tier I |
| 2 |
NC_000085.6 |
19 |
9008551 |
+ |
Ahnak |
NNNTGGAAGGAGACCTCAAA |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000081.6 |
15 |
101024041 |
- |
Scn8a |
NNNCGGAAGAAAACCACAAA |
NGG |
2 |
0.8711 |
Tier II |
| 4 |
NC_000086.7 |
X |
140929212 |
- |
Vsig1 |
NNNAGAAAGGAGACCACAAA |
NGG |
2 |
0.8421 |
Tier II |
| 5 |
NC_000067.6 |
1 |
167282044 |
+ |
Uck2 |
NNNCAGAAGGAGACCAAAAA |
NGG |
2 |
0.4044 |
Tier II |
| 6 |
NC_000073.6 |
7 |
56104714 |
- |
Herc2 |
NNNCTAAAGGAGACCACAAA |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000077.6 |
11 |
88931725 |
- |
Scpep1 |
NNNTTGAAGGAGACCACAAA |
NGG |
2 |
0.24 |
Tier II |
| 8 |
NC_000069.6 |
3 |
32848064 |
- |
Usp13 |
NNNCAGAAGGAGACCACCAA |
NGG |
2 |
0.1651 |
Tier II |
| 9 |
NC_000081.6 |
15 |
76450622 |
- |
Mroh1 |
NNNGGGAAGGAGACCCCAAA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000077.6 |
11 |
88931725 |
- |
Scpep1os |
NNNTTGAAGGAGACCACAAA |
NGG |
2 |
0.24 |
Tier III |
| 11 |
NC_000085.6 |
19 |
56097158 |
- |
Gm52387 |
NNNCGGAAGGAGACCACGAA |
NAG |
2 |
0.1037 |
Tier III |
Other clones with same target sequence:
(none)
