Construct: sgRNA BRDN0001148922
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCAGGTGACAAGTAGTATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK1 (4750)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76127
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
169588698 |
+ |
NEK1 |
NNNAGGTGACAAGTAGTATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
142119849 |
- |
NMBR |
NNNAAGTGACAAGTAGTATA |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000014.9 |
14 |
36994191 |
- |
SLC25A21 |
NNNAGGTAACAAGTAGTTTG |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000010.11 |
10 |
92064352 |
+ |
CPEB3 |
NNNAGGTGAGAAGTAGTATG |
NGG |
1 |
0.3889 |
Tier II |
| 5 |
NC_000005.10 |
5 |
131812487 |
+ |
MEIKIN |
NNNAGGGGTCAAGTAGTATG |
NGG |
2 |
0.3529 |
Tier II |
| 6 |
NC_000001.11 |
1 |
48564489 |
- |
AGBL4 |
NNNAGGGGCCAAGTAGTATG |
NGG |
2 |
0.3361 |
Tier II |
| 7 |
NC_000003.12 |
3 |
16485912 |
- |
RFTN1 |
NNNAGGTAACAAGTAGTAAG |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000016.10 |
16 |
81681415 |
- |
CMIP |
NNNAGGTCACAACTAGTATG |
NGG |
2 |
0.2591 |
Tier II |
| 9 |
NC_000017.11 |
17 |
51831412 |
+ |
CA10 |
NNNATGTGACAAGAAGTATG |
NGG |
2 |
0.1857 |
Tier II |
| 10 |
NC_000012.12 |
12 |
51344071 |
- |
CELA1 |
NNNAGGTGACATGTAGTTTG |
NGG |
2 |
0.1667 |
Tier II |
| 11 |
NC_000006.12 |
6 |
167326479 |
+ |
TTLL2 |
NNNAGGTGACAAGCAGAATG |
NGG |
2 |
0.1524 |
Tier II |
| 12 |
NC_000003.12 |
3 |
52527750 |
+ |
NT5DC2 |
NNNAGGTGGCAAGTAGTCTG |
NGG |
2 |
0.127 |
Tier II |
| 13 |
NC_000012.12 |
12 |
117262822 |
- |
NOS1 |
NNNAGCTGACAAGTAGGATG |
NGG |
2 |
0.0802 |
Tier II |
| 14 |
NC_000002.12 |
2 |
148088833 |
+ |
MBD5 |
NNNAGGTGACAACTAGTATG |
NTG |
2 |
0.0164 |
Tier II |
| 15 |
NC_000020.11 |
20 |
4230196 |
- |
ADRA1D |
NNNAGGTGACAAGTACCATG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000006.12 |
6 |
69687347 |
- |
LMBRD1 |
NNNAGGTGACAAGTATTATG |
NAG |
2 |
0.0 |
Tier II |
| 17 |
NC_000014.9 |
14 |
54990806 |
- |
WDHD1 |
NNNAGGTGGCAAGTACTATG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000003.12 |
3 |
84805280 |
- |
LINC00971 |
NNNAGGTGACAAGTAATATC |
NGG |
2 |
0.4286 |
Tier III |
| 19 |
NC_000002.12 |
2 |
219909460 |
+ |
LOC105373890 |
NNNAGGTGACCAGTAGTATA |
NGG |
2 |
0.375 |
Tier III |
| 20 |
NC_000003.12 |
3 |
16485912 |
- |
LOC124906219 |
NNNAGGTAACAAGTAGTAAG |
NGG |
2 |
0.2857 |
Tier III |
| 21 |
NC_000007.14 |
7 |
79652660 |
+ |
LOC105375369 |
NNNAGGTTACAAGTTGTATG |
NGG |
2 |
0.125 |
Tier III |
| 22 |
NC_000002.12 |
2 |
13579314 |
+ |
LOC105373438 |
NNNAGGTGACAAGTAGTAAG |
NGC |
2 |
0.0063 |
Tier III |
| 23 |
NC_000004.12 |
4 |
33911402 |
+ |
LOC101928622 |
NNNAGGTGACAGGTATTATG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
61020074 |
- |
Nek1 |
NNNAGGTGACAAGTAGTATG |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000071.6 |
5 |
53623779 |
- |
Rbpj |
NNNAGTTGACAAGTAGTATT |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000079.6 |
13 |
3609616 |
+ |
Tasor2 |
NNNAGGTAACAAGTAGTATC |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000074.6 |
8 |
14456746 |
- |
Dlgap2 |
NNNAGGTGACAACTAATATG |
NGG |
2 |
0.4211 |
Tier II |
| 5 |
NC_000073.6 |
7 |
100450566 |
- |
C2cd3 |
NNNAGGTAAGAAGTAGTATG |
NGG |
2 |
0.3889 |
Tier II |
| 6 |
NC_000076.6 |
10 |
59847328 |
+ |
Micu1 |
NNNAGGTGACAAGAAGTATG |
NGT |
2 |
0.01 |
Tier II |
| 7 |
NC_000075.6 |
9 |
111638849 |
- |
Stac |
NNNAGGTAACAAGGAGTATG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000068.7 |
2 |
62982883 |
- |
Kcnh7 |
NNNAGGTGAAAAGGAGTATG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000077.6 |
11 |
42310352 |
+ |
Gabra6 |
NNNAGTTGACAAGTACTATG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000071.6 |
5 |
45340539 |
+ |
D5Ertd615e |
NNNAGGTGACAAGTAGGATT |
NGG |
2 |
0.0824 |
Tier III |
| 11 |
NC_000068.7 |
2 |
62982883 |
- |
Gm52513 |
NNNAGGTGAAAAGGAGTATG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
