Construct: sgRNA BRDN0001148923
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATTGGAAAAGGTCGCTATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMPR1B (658)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77490
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
95129920 |
+ |
BMPR1B |
NNNTGGAAAAGGTCGCTATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
7218566 |
+ |
DLG4 |
NNNTGGAAGAGGTCGCTATG |
NAG |
2 |
0.1728 |
Tier I |
| 3 |
NC_000012.12 |
12 |
2305977 |
- |
CACNA1C |
NNNTGGAGAAGGTCGATATG |
NGG |
2 |
0.7333 |
Tier II |
| 4 |
NC_000019.10 |
19 |
9876285 |
+ |
OLFM2 |
NNNTGGAAAAGGTCGCCATG |
NGG |
1 |
0.7059 |
Tier II |
| 5 |
NC_000005.10 |
5 |
102969347 |
+ |
PAM |
NNNTGGAAAAGGTAGCTATG |
NGG |
1 |
0.35 |
Tier II |
| 6 |
NC_000016.10 |
16 |
67611722 |
+ |
CTCF |
NNNTGTAAAAGGTCGTTATG |
NGG |
2 |
0.2051 |
Tier II |
| 7 |
NC_000017.11 |
17 |
7218566 |
+ |
ACADVL |
NNNTGGAAGAGGTCGCTATG |
NAG |
2 |
0.1728 |
Tier II |
| 8 |
NC_000016.10 |
16 |
475291 |
- |
RAB11FIP3 |
NNNTGGAAAACGTCGCTATG |
NTG |
2 |
0.0167 |
Tier II |
| 9 |
NC_000008.11 |
8 |
95476765 |
+ |
CFAP418-AS1 |
NNNTGGAAAAGGTGGCTATG |
NAG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
141857539 |
- |
Bmpr1b |
NNNTGGAAAAGGCCGCTATG |
NCG |
2 |
0.0846 |
Tier I |
| 2 |
NC_000072.6 |
6 |
40884863 |
- |
Moxd2 |
NNNTTGAAAAGGTTGCTATG |
NGG |
2 |
0.22 |
Tier II |
| 3 |
NC_000081.6 |
15 |
71898705 |
+ |
Col22a1 |
NNNTGGAAATGGTCTCTATG |
NGG |
2 |
0.1261 |
Tier II |
| 4 |
NC_000079.6 |
13 |
13630466 |
- |
Lyst |
NNNTGGAAAAGGTCTCTATT |
NGG |
2 |
0.1 |
Tier II |
| 5 |
NC_000071.6 |
5 |
135209934 |
+ |
Baz1b |
NNNTGGAAAAGGTCTCTATG |
NGA |
2 |
0.0099 |
Tier II |
| 6 |
NC_000085.6 |
19 |
5099914 |
+ |
Rab1b |
NNNAGGAAAAGGTGGCTATG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000086.7 |
X |
135588066 |
+ |
Nxf7 |
NNNTGGAAAGGGTGGCTATG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000082.6 |
16 |
8998024 |
+ |
Gm30444 |
NNNTGGAAAAGGTCGATCTG |
NGG |
2 |
0.1905 |
Tier III |
| 9 |
NC_000080.6 |
14 |
6949478 |
- |
Gm32041 |
NNNTGGAGAAGGTCGGTATG |
NGG |
2 |
0.1128 |
Tier III |
Other clones with same target sequence:
(none)
