Construct: sgRNA BRDN0001148926

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACCTGTGTACATATCCCATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STRADA (92335)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76054

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 63710506 + STRADA NNNTGTGTACATATCCCATG NGG 0 1.0 Tier I
2 NC_000018.10 18 58953777 + ZNF532 NNNTGTGTACATATCCCACG NGA 2 0.0192 Tier I
3 NC_000008.11 8 14424539 + SGCZ NNNTGTGTATATATCCCATA NGG 2 0.8824 Tier II
4 NC_000011.10 11 30523008 - MPPED2 NNNTGTGAACATGTCCCATG NGG 2 0.5217 Tier II
5 NC_000019.10 19 13285551 - CACNA1A NNNTGTGTTAATATCCCATG NGG 2 0.52 Tier II
6 NC_000011.10 11 67901513 - LOC112268076 NNNTGTGTCCAAATCCCATG NGG 2 0.4571 Tier II
7 NC_000022.11 22 32732659 + SYN3 NNNTGTCTACATATCCTATG NGG 2 0.3208 Tier II
8 NC_000017.11 17 58392133 - RNF43 NNNTGTGTGCATATCCCGTG NGG 2 0.2667 Tier II
9 NC_000018.10 18 26273392 - TAF4B NNNTGTGTACATATCACATG NAG 2 0.2593 Tier II
10 NC_000016.10 16 5567253 + RBFOX1 NNNTGTGTGGATATCCCATG NGG 2 0.2593 Tier II
11 NC_000003.12 3 192433821 - FGF12 NNNTGTGAACATACCCCATG NGG 2 0.2286 Tier II
12 NC_000019.10 19 11016136 + SMARCA4 NNNTGTGTAGAGATCCCATG NGG 2 0.1944 Tier II
13 NC_000008.11 8 41992578 - KAT6A NNNTGTGTACATATCCCTCG NGG 2 0.1379 Tier II
14 NC_000013.11 13 111233743 + ARHGEF7 NNNTGTGTACATATCACATG NCG 2 0.1071 Tier II
15 NC_000006.12 6 40436360 + LRFN2 NNNTGTTTACATATCGCATG NGG 2 0.0879 Tier II
16 NC_000007.14 7 131189000 + MKLN1 NNNTGTGTACATATCACATG NGA 2 0.0694 Tier II
17 NC_000004.12 4 140944398 - RNF150 NNNTGTGTACATCTACCATG NGG 2 0.0468 Tier II
18 NC_000003.12 3 23326754 + UBE2E2 NNNTGTGTACATGTCCCATG NTG 2 0.0254 Tier II
19 NC_000011.10 11 14882468 - PDE3B NNNTGTGTATATATCCCATG NGC 2 0.0209 Tier II
20 NC_000011.10 11 14882468 - CYP2R1 NNNTGTGTATATATCCCATG NGC 2 0.0209 Tier II
21 NC_000009.12 9 68836604 - PIP5K1B NNNTGTGTACATATCCCATA NGC 2 0.0208 Tier II
22 NC_000004.12 4 90691556 + CCSER1 NNNTGTGTACATATCACATG NGT 2 0.0161 Tier II
23 NC_000016.10 16 47298994 - ITFG1 NNNTGTGTACATCTGCCATG NGG 2 0.0105 Tier II
24 NC_000008.11 8 33440435 + POFUT3 NNNTGTGTACATATCTCATG NGC 2 0.0068 Tier II
25 NC_000005.10 5 111810214 + NREP NNNTGTGTACAGAGCCCATG NGG 2 0.0 Tier II
26 NC_000018.10 18 52793164 + DCC NNNTGTTTACATAGCCCATG NGG 2 0.0 Tier II
27 NC_000004.12 4 18607342 + LOC105374510 NNNTATGTACACATCCCATG NGG 2 0.8211 Tier III
28 NC_000004.12 4 101730685 - LOC107986297 NNNTCTGTTCATATCCCATG NGG 2 0.4714 Tier III
29 NC_000003.12 3 130195834 - LOC107986133 NNNTGTGTCCAAATCCCATG NGG 2 0.4571 Tier III
30 NC_000011.10 11 3527537 - LOC101927708 NNNTGTGTCCAAATCCCATG NGG 2 0.4571 Tier III
31 NC_000001.11 1 75991509 + LOC105378806 NNNTGTCTTCATATCCCATG NGG 2 0.4125 Tier III
32 NC_000023.11 X 23196791 + PTCHD1-AS NNNTGTGTACATAACCCATC NGG 2 0.2653 Tier III
33 NC_000010.11 10 77734004 + LOC399783 NNNTGTGTACATATCCCACG NGA 2 0.0192 Tier III
34 NC_000016.10 16 47298994 - ITFG1-AS2 NNNTGTGTACATCTGCCATG NGG 2 0.0105 Tier III
35 NC_000010.11 10 15761164 + LOC124902383 NNNTGTGTACATATGCCATG NTG 2 0.0019 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 6796847 + Zfp804b NNNTGTGTAAATATCCCATA NGG 2 0.8125 Tier II
2 NC_000068.7 2 106756624 + Mpped2 NNNTGTGAACATGTCCCATG NGG 2 0.5217 Tier II
3 NC_000077.6 11 101428606 + Rundc1 NNNTGTGTACATATCACATC NGG 2 0.4286 Tier II
4 NC_000070.6 4 25221053 - Fhl5 NNNTGTTGACATATCCCATG NGG 2 0.419 Tier II
5 NC_000067.6 1 136851894 - Nr5a2 NNNTGTGTTCATGTCCCATG NGG 2 0.3913 Tier II
6 NC_000074.6 8 3631600 + Stxbp2 NNNTGTGCACATACCCCATG NGG 2 0.2857 Tier II
7 NC_000085.6 19 46134897 - Elovl3 NNNAGGGTACATATCCCATG NGG 2 0.2721 Tier II
8 NC_000075.6 9 48267075 - Nxpe4 NNNTGTGCACATATCCCATG NAG 2 0.2593 Tier II
9 NC_000084.6 18 30341324 + Pik3c3 NNNTGTGTGCATATCCCATG NAG 2 0.1728 Tier II
10 NC_000083.6 17 30444889 + Btbd9 NNNTGTGTAGCTATCCCATG NGG 2 0.1556 Tier II
11 NC_000075.6 9 96891087 - Pxylp1 NNNTGTCTACATATCCCATG NGA 2 0.0477 Tier II
12 NC_000083.6 17 80565780 + Cdkl4 NNNTGTGTACATCTCCCCTG NGG 2 0.0401 Tier II
13 NC_000071.6 5 101732875 + Gm42134 NNNTGGGTACATATTCCATG NGG 2 0.0381 Tier II
14 NC_000076.6 10 40423665 - Cdk19 NNNTGTGTACATATCCCATT NTG 2 0.0273 Tier II
15 NC_000080.6 14 72707110 - Fndc3a NNNTGTGTACATTTGCCATG NGG 2 0.015 Tier II
16 NC_000083.6 17 42407766 + Ptchd4 NNNTGTGTACAAATCCCATG NGT 2 0.0129 Tier II
17 NC_000077.6 11 72717195 - Ankfy1 NNNTGTGTACATATGCCATG NTG 2 0.0019 Tier II
18 NC_000082.6 16 4775964 + Cdip1 NNNTGTGTACATATGCCATG NTG 2 0.0019 Tier II
19 NC_000073.6 7 132660648 + Lhpp NNNTGTGTACATATGCCATG NGC 2 0.0011 Tier II
20 NC_000070.6 4 19428214 - Cngb3 NNNTGTGTACATATGCCATG NGT 2 0.0008 Tier II
21 NC_000072.6 6 21693502 - Kcnd2 NNNTGTGTACATAGCACATG NGG 2 0.0 Tier II
22 NC_000081.6 15 53877885 + Samd12 NNNTGTGTACATATCCCATG NAT 2 0.0 Tier II
23 NC_000070.6 4 54888579 - Gm34133 NNNTGTGTACTTATCACATG NGG 2 0.3077 Tier III
24 NC_000075.6 9 48267075 - Gm51672 NNNTGTGCACATATCCCATG NAG 2 0.2593 Tier III
25 NC_000077.6 11 25622203 - 5730522E02Rik NNNTGTGAACATATCCCAAG NGG 2 0.2286 Tier III
26 NC_000071.6 5 101732875 + 9430085M18Rik NNNTGGGTACATATTCCATG NGG 2 0.0381 Tier III
27 NC_000074.6 8 107213029 - 4930517G24Rik NNNTGTGAACATATCCCATG NTG 2 0.0312 Tier III
28 NC_000069.6 3 103726964 - LOC108168889 NNNTGTGTTCATATCCCATG NGC 2 0.0133 Tier III
29 NC_000076.6 10 98844976 + Gm34346 NNNTGTGTACATATTCCATG NGC 2 0.0015 Tier III
30 NC_000072.6 6 39244819 + Gm38794 NNNTGTGTACATATGCCATG NGT 2 0.0008 Tier III
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Other clones with same target sequence:

(none)