Construct: sgRNA BRDN0001148929
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTACTGCCAGGCTATTACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5K1B (8395)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77086
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
68889129 |
+ |
PIP5K1B |
NNNACTGCCAGGCTATTACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
100181133 |
+ |
ADAMTS17 |
NNNACTGCCAGGCTACCACA |
NGG |
2 |
0.4706 |
Tier II |
| 3 |
NC_000003.12 |
3 |
62420095 |
+ |
CADPS |
NNNATTTCCAGGCTATTACA |
NGG |
2 |
0.3636 |
Tier II |
| 4 |
NC_000002.12 |
2 |
166065177 |
- |
SCN1A |
NNNACTTCCAGGCTATTTCA |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000010.11 |
10 |
47905949 |
+ |
ANXA8 |
NNNACTGCCACGCTGTTACA |
NGG |
2 |
0.2786 |
Tier II |
| 6 |
NC_000006.12 |
6 |
150207953 |
- |
PPP1R14C |
NNNTCTGCCATGCTATTACA |
NGG |
2 |
0.2448 |
Tier II |
| 7 |
NC_000002.12 |
2 |
238250379 |
- |
PER2 |
NNNTCTGCCAGGCTAGTACA |
NGG |
2 |
0.2203 |
Tier II |
| 8 |
NC_000007.14 |
7 |
133429766 |
- |
EXOC4 |
NNNACTGCCATGCTATAACA |
NGG |
2 |
0.2051 |
Tier II |
| 9 |
NC_000012.12 |
12 |
128580474 |
- |
TMEM132C |
NNNCCTGCCAGGCTATTTCA |
NGG |
2 |
0.1765 |
Tier II |
| 10 |
NC_000003.12 |
3 |
113895654 |
- |
GRAMD1C |
NNNACTGCCATGCTATTACA |
NAG |
2 |
0.0997 |
Tier II |
| 11 |
NC_000013.11 |
13 |
26174788 |
- |
RNF6 |
NNNACTGCCAGGATTTTACA |
NGG |
2 |
0.0769 |
Tier II |
| 12 |
NC_000022.11 |
22 |
20563347 |
- |
MED15 |
NNNACTGCCAGGGTATTCCA |
NGG |
2 |
0.026 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
24381693 |
- |
Pip5k1b |
NNNGCTGCCGGGCTATTACA |
NGG |
2 |
0.3472 |
Tier I |
| 2 |
NC_000067.6 |
1 |
185249935 |
- |
Rab3gap2 |
NNNGCTGCCAGGGTATTACA |
NGG |
2 |
0.0852 |
Tier I |
| 3 |
NC_000070.6 |
4 |
94650935 |
+ |
Ift74 |
NNNACTGCCAAGTTATTACA |
NGG |
2 |
0.7 |
Tier II |
| 4 |
NC_000069.6 |
3 |
152824652 |
+ |
St6galnac5 |
NNNTCTGCCATGCTATTACA |
NGG |
2 |
0.2448 |
Tier II |
| 5 |
NC_000069.6 |
3 |
152824652 |
+ |
Pigk |
NNNTCTGCCATGCTATTACA |
NGG |
2 |
0.2448 |
Tier II |
| 6 |
NC_000079.6 |
13 |
77766432 |
+ |
Fam172a |
NNNACTGCCTGGCTCTTACA |
NGG |
2 |
0.2406 |
Tier II |
| 7 |
NC_000070.6 |
4 |
100989018 |
- |
Cachd1 |
NNNACTGCCTGGCTTTTACA |
NGG |
2 |
0.1765 |
Tier II |
| 8 |
NC_000075.6 |
9 |
48722513 |
+ |
Zbtb16 |
NNNACTGCCAGGCTGTTACC |
NGG |
2 |
0.1477 |
Tier II |
| 9 |
NC_000074.6 |
8 |
125843563 |
- |
Pcnx2 |
NNNACTGTCAGGCTATTAGA |
NGG |
2 |
0.1094 |
Tier II |
| 10 |
NC_000079.6 |
13 |
20907858 |
- |
Aoah |
NNNACTGCCAGGCTATGACA |
NGT |
2 |
0.0019 |
Tier II |
Other clones with same target sequence:
(none)
